Introduction
Methods and materials
Target virusesa | Target genesb | Primer sequence, 5’-3’c | Size (bp) | Source or reference |
---|---|---|---|---|
PEDV | S | F:TTCTGAGTCACGAACAGCCA | 651 | [19] |
R:CATATGCAGCCTGCTCTGAA | ||||
TGEV | S | F:GTGGTTTTGGTYRTAAATGC | 859 | [19] |
R:CACTAACCAACGTGGARCTA | ||||
RVA | VP6 | F: AAAGATGCTAGGGACAAAATTG | 308 | [20] |
R: TTCAGATTGTGGAGCTATTCCA | ||||
nF:GACAAAATTGTCGAAGGCACATTATA | 121 | |||
nR: TCGGTAGATTACCAATTCCTCCAG |
Results
District | Infection rate (%) | PToV and PEDV Co-infection | PToV and RVA Co-infection | PToV and TGEV Co-infection | PToV, RVA, TGEV and PEDV Co-infection | |||
---|---|---|---|---|---|---|---|---|
PToV | PEDV | RVA | TGEV | |||||
Meishan | 47.1%(64/136) | 64%(87/136) | 67.6%(92/136) | 13.2%(18/136) | 47.1%(64/136) | 47.1%(64/136) | 12.5%(17/136) | 3.7%(5/136) |
Leshan | 5.77%(3/52) | 71.1%(37/52) | 78.8%(41/52) | 15.4%(8/52) | 5.77%(3/52) | 5.77%(3/52) | 15.4%(8/52) | 3.8%(2/52) |
Mianyang | 51.9%(69/133) | 74.4%(99/133) | 73.7%(98/133) | 15%(20/133) | 50.4%(67/133) | 48.9%(65/133) | 15%(20/133) | 6%(8/133) |
Ya’an | 36.5%(42/115) | 76.5%(88/115) | 79.1%(91/115) | 0 | 36.5%(42/115) | 31.3%(36/115) | 0 | 0 |
Dazhou | 9.5%(2/21) | 52.4%(11/21) | 61.9%(13/21) | 0 | 0 | 9.5%(2/21) | 0 | 0 |
Ziyang | 10.3%(4/39) | 66.7%(26/39) | 64.1%(25/39) | 10.3%(4/39) | 10.3%(4/39) | 10.3%(4/39) | 7.7%(3/39) | 5.1%(2/39) |
Yibin | 38.9%(21/54) | 0 | 63%(34/54) | 13%(7/54) | 0 | 35.2%(19/54) | 13%(7/54) | 0 |
Chengdu | 48.8%(40/82) | 67.1%(55/82) | 81.7%(67/82) | 18.3%(15/82) | 47.6%(39/82) | 43.9%(36/82) | 14.6%(12/82) | 4.9%(4/82) |
Deyang | 41.8%(43/103) | 74.8%(77/103) | 69.9%(72/103) | 15.5%(16/103) | 26.2%(27/103) | 32%(33/103) | 15.5%(16/103) | 7.8%(8/103) |
Luzhou | 4%(1/25) | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Suining | 42.1%(32/76) | 63.2%(48/76) | 65.8%(50/76) | 25%(19/76) | 35.5%(27/76) | 42.1%(32/76) | 25%(19/76) | 9.2%(7/76) |
Zigong | 27.8%(10/36) | 47.2%(17/36) | 0 | 5.6%(2/36) | 22.2%(8/36) | 0 | 0 | 0 |
No. | Name | Isolation year | Area | Accession no. | No. | Name | Isolation year | Area | Accession no. |
---|---|---|---|---|---|---|---|---|---|
1 | BToV -B150 | 2003 | Netherlands | AJ575376 | 21 | FJ-BRES | 2009 | Spain | FJ232069 |
2 | BToV -B155 | 2003 | Netherlands | AJ575377 | 22 | SC-2011-B1 | 2011 | Mianyang | KF727568 |
3 | AJ-strain_P4 | 2003 | Netherlands | AJ575369 | 23 | SC-2011-B2 | 2011 | Mianyang | KF727569 |
4 | AJ-strain_P9 | 2003 | Netherlands | AJ575370 | 24 | SC-2011-E | 2011 | Dazhou | KF727574 |
5 | AJ-strain_P10 | 2003 | Netherlands | AJ575371 | 25 | SC-2011-A1 | 2011 | Meishan | KF727566 |
6 | BToV -B6 | 2003 | Netherlands | AJ575374 | 26 | SC-2011-A2 | 2011 | Meishan | KF727567 |
7 | AJ-strain_M | 2003 | Netherlands | AJ575368 | 27 | SC-2011-F | 2011 | Ziyang | KF727575 |
8 | Breda virus-1 | 2005 | Canada | AF076621 | 28 | SC-2012-L1 | 2012 | Ya’an | KF727579 |
9 | Breda virus-2 | 2005 | Canada | AY427798 | 29 | SC-2012-L2 | 2012 | Ya’an | KF727580 |
10 | GU07-109-15 | 2009 | Korea | GU181240 | 30 | SC-2012-C1 | 2012 | Chengdu | KF727570 |
11 | GU07-109-14 | 2009 | Korea | GU181241 | 31 | SC-2012-C2 | 2012 | Chengdu | KF727571 |
12 | GU07-109-13 | 2009 | Korea | GU181242 | 32 | SC-2012-D1 | 2012 | Deyang | KF727572 |
13 | GU07-109-12 | 2009 | Korea | GU181243 | 33 | SC-2012-D2 | 2012 | Deyang | KF727573 |
14 | GU07-109-11 | 2009 | Korea | GU181244 | 34 | SC-2012-H1 | 2012 | Yibin | KF727577 |
15 | GU-07-56-23 | 2009 | Korea | GU181245 | 35 | SC-2012-H2 | 2012 | Yibin | KF727578 |
16 | GU-07-56-11 | 2009 | Korea | GU181248 | 36 | SC-2012-K | 2012 | Luzhou | KF727582 |
17 | GU-07-55-5 | 2009 | Korea | GU181249 | 37 | SC-2012-G | 2012 | Suining | KF727576 |
18 | GU-07-55-4 | 2009 | Korea | GU181250 | 38 | SC-2012-J | 2012 | Zigong | KF727581 |
19 | GU-07-56-14 | 2009 | Korea | GU181247 | 39 | SC-2013-I1 | 2013 | Leshan | KF727573 |
20 | GU-07-56-22 | 2009 | Korea | GU181246 | 40 | SC-2012-I2 | 2013 | Leshan | KF727574 |
District
|
Name
|
Nucleotide substitutions
| |||||||||||||||||||||||||
1
|
1
|
2
|
3
|
7
|
9
|
1
|
1
|
1
|
1
|
1
|
2
|
2
|
2
|
2
|
3
|
3
|
4
|
4
|
4
|
4
|
4
|
4
|
4
|
5
|
5
| ||
7
|
8
|
7
|
9
|
2
|
3
|
1
|
2
|
2
|
5
|
7
|
2
|
2
|
7
|
8
|
0
|
8
|
0
|
2
|
3
|
4
|
4
|
4
|
9
|
1
|
1
| ||
1
|
1
|
3
|
1
|
2
|
2
|
8
|
6
|
8
|
6
|
4
|
8
|
3
|
2
|
1
|
2
|
7
|
5
|
2
|
3
| ||||||||
Meishan | SC-2011-A1 | T | T | C | A | A | C | - | C | T | C | - | T | - | T | G | C | C | - | C | - | - | C | - | T | G | C |
SC-2011-A2 | T | T | C | A | A | C | C | C | T | C | T | T | - | T | G | C | C | - | C | - | - | C | - | T | G | C | |
Mianyang | SC-2011-B1 | T | T | C | A | A | C | C | C | C | C | T | T | - | T | G | C | C | - | C | - | - | C | - | T | G | C |
SC-2011-B2 | T | T | C | A | A | C | C | C | T | C | T | T | - | T | G | C | C | - | C | - | - | C | - | T | G | C | |
Dazhou | SC-2011-E | T | T | C | A | A | C | C | C | T | C | T | T | - | T | G | C | C | - | C | - | - | C | - | T | G | C |
Ziyang | SC-2011-F | T | T | C | A | A | C | C | C | T | C | T | T | C | T | G | C | C | - | C | - | - | C | - | T | G | C |
Chengdu | SC-2012-C1 | T | T | C | A | A | - | C | C | T | - | T | T | - | T | G | C | C | - | C | - | - | C | - | T | G | C |
SC-2012-C2 | T | T | C | A | A | C | C | C | T | - | T | T | C | T | G | C | C | - | C | - | - | C | - | T | G | C | |
Deyang | SC-2012-D1 | T | T | C | A | A | C | C | C | C | C | - | T | C | T | G | C | C | T | - | - | - | C | - | T | G | C |
SC-2012-D2 | T | T | C | A | A | C | C | C | C | C | - | T | C | T | G | C | C | T | - | - | - | C | - | T | G | C | |
Suining | SC-2012-G | T | T | C | A | A | C | C | C | C | C | - | T | C | T | G | C | C | T | - | - | - | C | - | T | G | C |
Zigong | SC-2012-J | T | T | C | A | A | C | C | C | C | C | T | - | - | T | G | C | - | T | C | - | - | C | - | T | G | C |
Luzhou | SC-2012-K | T | T | C | A | A | C | C | C | T | C | T | - | - | T | G | C | - | T | C | T | A | C | T | T | G | C |
Yibin | SC-2012-H1 | T | T | C | A | A | C | - | C | T | C | - | - | - | T | G | C | - | - | - | T | - | C | - | T | G | C |
SC-2012-H2 | T | T | C | A | A | C | - | C | T | C | - | - | - | T | G | C | - | - | C | T | - | C | - | T | G | C | |
Ya’an | SC-2012-L1 | T | T | C | A | A | - | C | C | C | C | - | T | - | T | G | C | - | T | C | - | A | C | T | T | G | C |
SC-2012-L2 | T | T | C | A | A | C | C | C | C | C | - | T | - | T | G | C | - | T | C | - | A | C | T | T | G | C | |
Leshan | SC-2013-I1 | T | T | C | A | A | C | - | C | T | C | T | - | - | T | G | C | - | - | C | T | - | C | - | T | G | C |
SC-2013-I2 | T | T | C | A | A | C | - | C | T | C | T | - | - | T | G | C | - | - | C | T | - | C | - | T | G | C | |
Korea | GU181244 | A | C | T | T | G | T | T | T | G | T | C | T | T | C | A | T | T | A | T | G | G | T | C | C | A | T |
District
|
Name
|
Nucleotide substitutions
| |||||||||||||||||||||||||
5
|
5
|
5
|
5
|
5
|
5
|
5
|
5
|
5
|
5
|
6
|
6
|
6
|
6
|
6
|
6
|
6
|
6
|
6
|
6
|
6
|
6
|
6
|
6
|
6
| |||
2
|
3
|
3
|
5
|
6
|
6
|
7
|
8
|
8
|
9
|
0
|
0
|
1
|
1
|
1
|
3
|
3
|
3
|
5
|
5
|
5
|
6
|
6
|
7
|
9
| |||
8
|
1
|
7
|
2
|
0
|
1
|
9
|
3
|
5
|
4
|
3
|
6
|
2
|
5
|
8
|
3
|
6
|
9
|
2
|
4
|
7
|
3
|
6
|
8
|
0
| |||
Meishan | SC-2011-A1 | - | - | C | T | G | T | T | - | - | - | C | - | G | - | T | T | A | T | T | G | G | C | T | G | T | |
SC-2011-A2 | - | - | C | T | G | T | T | - | - | - | C | - | G | - | T | T | A | T | T | G | G | C | T | G | T | ||
Mianyang | SC-2011-B1 | - | - | C | T | G | T | T | C | G | - | C | C | G | - | T | T | A | T | T | G | G | C | T | G | T | |
SC-2011-B2 | - | - | C | T | G | T | T | C | G | - | - | C | G | - | T | T | A | T | T | G | G | C | T | G | T | ||
Dazhou | SC-2011-E | T | C | C | T | G | T | T | - | - | - | C | - | G | - | T | T | A | T | T | G | G | C | T | G | T | |
Ziyang | SC-2011-F | - | - | C | T | G | T | T | C | G | - | C | - | A | T | T | T | A | T | T | G | G | C | T | G | T | |
Chengdu | SC-2012-C1 | - | - | C | T | G | T | T | C | G | - | C | - | A | T | T | T | A | T | T | G | G | C | T | G | T | |
SC-2012-C2 | - | - | C | T | G | T | T | C | G | - | C | - | A | T | T | T | A | T | T | G | G | C | T | G | T | ||
Deyang | SC-2012-D1 | - | - | C | T | G | T | T | - | - | - | C | - | A | G | T | T | A | T | T | G | G | C | T | G | T | |
SC-2012-D2 | - | - | C | T | G | T | T | - | - | - | C | - | G | - | T | T | A | T | T | G | G | C | T | G | T | ||
Suining | SC-2012-G | - | - | C | T | G | T | T | C | G | C | C | - | A | G | T | T | A | T | T | G | G | C | T | G | T | |
Zigong | SC-2012-J | T | C | C | T | - | T | T | - | G | - | A | T | G | - | T | T | A | - | T | G | G | C | T | G | T | |
Luzhou | SC-2012-K | T | C | C | T | - | T | T | - | G | - | C | T | G | - | T | T | A | T | T | G | G | C | T | G | T | |
Yibin | SC-2012-H1 | - | - | C | T | G | T | T | - | - | C | A | T | A | T | T | T | A | - | T | G | G | C | T | G | T | |
SC-2012-H2 | - | - | C | T | G | T | T | C | G | C | A | T | A | T | T | T | A | T | T | G | G | C | T | G | T | ||
Ya’an | SC-2012-L1 | T | C | C | T | G | T | T | - | - | C | A | T | G | - | T | T | A | T | T | G | G | C | T | G | T | |
SC-2012-L2 | T | C | C | T | G | T | T | - | - | C | A | T | G | G | T | T | A | T | T | G | G | C | T | G | T | ||
Leshan | SC-2013-I1 | - | - | C | T | G | T | T | C | G | C | A | T | A | T | T | T | A | - | T | G | G | C | T | G | T | |
SC-2013-I2 | - | - | C | T | G | T | T | - | G | C | A | T | A | T | T | T | A | - | T | G | G | C | T | G | T | ||
Korea | GU181244 | A | T | T | A | A | C | C | A | A | T | T | A | T | A | C | C | G | C | C | T | A | T | A | A | A |