Electronic supplementary material
MT in cell growth
MT in drug resistance
MT functions in hematopoietic cells
ES-derived differentiated cells
Overexpression of MT-I gene prevents LIF withdrawal induced apoptosis in ES derived differentiated cells
human cord blood cells
MT is more highly expressed in mature CD34– cells than in immature CD34+ cells
Megakaryocytic DAMI cells
Overexpression of MT-IIA resulted in the increase of cell size, intracellular granulation and increment of the megakaryocytic specific CD41 and CD42 and arresting in cell proliferation
K562, DAMI, MEG-01, ELF-153
The more mature K562, DAMI, and MEG-01 cell lines exhibited transcription of all MT isogenes, except MT-III and MT-IV.
Phorbol ester induces increased MT transcription and biosynthesis.
EPO- or sodium butyrate-induced differentiation as monitored by hemoglobin formation was inhibited in K562 cells stably transfected with an expression vector containing human MT-IIA gene.
Rat (acute blood loss, phenylhydrazine induced anemia model)
Induced erythropoiesis or anemia in rats, the induction of MT in the bone marrow is enhanced, with predominant accumulation in erythroblasts
T cell line CCRF-CEM
Inhibition of hMTs synthesis by ODNs stimulated the apoptotic process
Expression analyses of MT in leukemia
Type of diseases
Number of patients analyzed
Results of the analysis
119 (initial: n = 92, relapsed: n = 27)
Tendency in initial ALL with MT expression, the lower probability of disease free survival. However, no differences concerning MT percentage of positivity and intensity of staining in children with either initial or relapsed ALL. MT expression is not an important prognostic factor in the clinical drug resistance of childhood ALL.
After chemotherapy, MT positive cases (n = 18) showed maximal effect on the second day of treatment and apoptotic action completed on the tenth day. MT negative cases showed maximal effect on the first day of treatment and completed on the sixth day.
From microarray CGH analysis, BAK, CDKN2C, GSTM1 and MT-IF as a gene set that differed between ALL patients at diagnosis who had a risk or relapse from those who did not.
The expression of resistance-related proteins P-170, GST-pi, Topo II, TS and MT was investigated. Patients who developed a relapse expressed more than two resistance mechanisms significantly more often than patients who remained remission (p = 0.005). The higher the number of resistance-related proteins in childhood AML the poorer the prognosis of the patients.
mRNA expressions of the MT-IA, G and PU.1 genes were significantly, inversely correlated (MT-IG: R = −0.50, p < 0.001; MT-IA: R = −0.58, p < 0.0005).
MT is differentially expressed in subclassified Hodgkin lymphoma. The number of MT-I, II immunostained cells is significantly higher in MCHL relative to other subclassified HL groups (p < 0.001), and also, the number of these cells is significantly higher in NSHL relative to NLPHL and LRCHL (p < 0.005).
MT labeling of more than 20% lymphoma cells was associated with a significantly poorer 5-year survival, independent of age, stage, or international prognostic index.
OMF (n = 9), CML (n = 11)
Increased GST-pi and MT expression in the bone marrow of MPD patients. Levels of MT in OMF patients were higher than in CML.