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European Journal of Clinical Microbiology & Infectious Diseases

Erschienen in:

01.12.2014 | Review

Molecular revolution in the diagnosis of microbial brain abscesses

verfasst von: A. K. Mishra, H. Dufour, P.-H. Roche, M. Lonjon, D. Raoult, P.-E. Fournier

Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases | Ausgabe 12/2014

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Abstract

A brain abscess is a life-threatening infection, frequently with serious sequelae. Culture-based methods present many limitations and do not enable an exhaustive documentation of the bacterial flora. 16S rRNA-based amplification, cloning, and high-throughput sequencing have dramatically increased the number of identified agents of brain abscesses, showing that the causative flora is polymicrobial in up to 40 % of cases, with the presence of at least one anaerobic bacterium. In contrast, real-time polymerase chain reaction (PCR) technology is an appealing alternative to culture-based methods for diagnosing brain abscesses due to its speed, sensitivity, and specificity. Molecular typing is available for several bacterial and fungal genera, and this user-friendly tool is accessible for the clinical microbiology laboratory to diagnose microbes involved in a brain abscess. This article reviews the applications of the currently available tools for the etiological diagnosis of a brain abscess.

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Titel: Molecular revolution in the diagnosis of microbial brain abscesses
verfasst von: A. K. Mishra
H. Dufour
P.-H. Roche
M. Lonjon
D. Raoult
P.-E. Fournier
Publikationsdatum: 01.12.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases / Ausgabe 12/2014
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-014-2166-z

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