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Erschienen in: Journal of Parasitic Diseases 3/2018

15.05.2018 | Original Article

Morphological and molecular characterization of entomopathogenic nematode, Heterorhabditis baujardi (Rhabditida, Heterorhabditidae) from Mizoram, northeastern India

verfasst von: Vanlalhlimpuia, Lalramliana, Hrang Chal Lalramnghaki, Vanramliana

Erschienen in: Journal of Parasitic Diseases | Ausgabe 3/2018

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Abstract

Isolation of entomopathogenic nematodes from Mizoram, northeastern part of India includes a Heterorhabditis species never been recorded in India. The morphological and multigene (ITS rRNA, 28S rRNA and COI) sequences analysis had revealed that the isolated Heterorhabditis belongs to Heterorhabditis baujardi, originally described from Vietnam. The phylogenetic tree (both MP and ML) revealed that the H. baujardi belongs to H. indica clade, and further, forms a monophyletic clade (99/98% and 94/92% bootstrap support for ITS and 28S respectively) with H. amazonensis, H. floridensis, H. mexicana and H. taysearae forming H. baujardi sub clade. The multigene characterization revealed that both the ITS and 28S rRNA showed similar result in resolving the phylogenetic relationship of the genus Heterorhabditis, with the ITS rRNA being superior based on the strong bootstrap support, whereas, the cytochrome c oxidase I (M1–M6 partition) can be a good supportive tool for species delimitation. This is the first report of H. baujardi from India envisaging its future use as a biological control agent, and further incorporated into the IPM.
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Metadaten
Titel
Morphological and molecular characterization of entomopathogenic nematode, Heterorhabditis baujardi (Rhabditida, Heterorhabditidae) from Mizoram, northeastern India
verfasst von
Vanlalhlimpuia
Lalramliana
Hrang Chal Lalramnghaki
Vanramliana
Publikationsdatum
15.05.2018
Verlag
Springer India
Erschienen in
Journal of Parasitic Diseases / Ausgabe 3/2018
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-018-1004-0

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