The online version of this article (doi:10.1186/1475-2875-11-407) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
PJS, WJ and JL designed the study; PJS and WJ developed the protocol; PJS, WJ, PL and WN performed the experiments; PJS analysed the data; PJS wrote the manuscript; all authors read and approved the final manuscript.
Serial blood passage of Plasmodium increases virulence, whilst mosquito transmission inherently regulates parasite virulence within the mammalian host. It is, therefore, imperative that all aspects of experimental malaria research are studied in the context of the complete Plasmodium life cycle.
Plasmodium chabaudi chabaudi displays many characteristics associated with human Plasmodium infection of natural mosquito vectors and the mammalian host, and thus provides a unique opportunity to study the pathogenesis of malaria in a single infection setting. An optimized protocol that permits efficient and reproducible vector transmission of P. c. chabaudi via Anopheles stephensi was developed.
This protocol was utilized for mosquito transmission of genetically distinct P. c. chabaudi isolates, highlighting differential parasite virulence within the mosquito vector and the spectrum of host susceptibility to infection initiated via the natural route, mosquito bite. An apposite experimental system in which to delineate the pathogenesis of malaria is described in detail.
Landau I, Boulard Y: Life cycles and morphology. Rodent Malaria. Edited by: Killick-Kendrick R, Peters W. 1978, (London) Ltd: Academic Press Inc, 53-84.
Malaria Research and Reference Reagent Resource Center. [ http://www.mr4.org]
Beier JC, Davis JR, Vaughan JA, Noden BH, Beier MS: Quantitation of Plasmodium falciparum sporozoites transmitted in vitro by experimentally infected Anopheles gambiae and Anopheles stephensi. AmJTrop Med Hyg. 1991, 44: 564-570.
Jarra W, Hills LA, March JC, Brown KN: Protective immunity to malaria. Studies with cloned lines of Plasmodium chabaudi chabaudi and P. berghei in CBA/Ca mice. II. The effectiveness and inter- or intra-species specificity of the passive transfer of immunity with serum. Parasite Immunol. 1986, 8: 239-254. 10.1111/j.1365-3024.1986.tb01036.x. CrossRefPubMed
Epstein JE, Tewari K, Lyke KE, Sim BK, Billingsley PF, Laurens MB, Gunasekera A, Chakravarty S, James ER, Sedegah M, Richman A, Velmurugan S, Reyes S, Li M, Tucker K, Ahumada A, Ruben AJ, Li T, Stafford R, Eappen AG, Tamminga C, Bennett JW, Ockenhouse CF, Murphy JR, Komisar J, Thomas N, Loyevsky M, Birkett A, Plowe CV, Loucq C, Edelman R, Richie TL, Seder RA, Hoffman SL: Live attenuated malaria vaccine designed to protect through hepatic CD8+ T cell immunity. Science. 2011, 334: 475-480. 10.1126/science.1211548. CrossRefPubMed
- Mosquito transmission of the rodent malaria parasite Plasmodium chabaudi
Philip J Spence
- BioMed Central
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