Skip to main content
main-content

01.12.2012 | Research | Ausgabe 1/2012 Open Access

Reproductive Biology and Endocrinology 1/2012

MT3 melatonin binding site, MT1 and MT2 melatonin receptors are present in oocyte, but only MT1 is present in bovine blastocyst produced in vitro

Zeitschrift:
Reproductive Biology and Endocrinology > Ausgabe 1/2012
Autoren:
Rafael V Sampaio, Dhúllia Stefanne B Conceição, Moysés S Miranda, Lucia de Fatima S Sampaio, Otávio Mitio Ohashi
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1477-7827-10-103) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

RVS carried out the autoradiographic binding and IVP assays, and helped to draft the manuscript. SDBC carried out IVP and immunoassays. MSM participated in coordination, immunoassays, performed the statistical analyses and helped to draft the manuscript. OMO participated in its design and coordination and critically review the manuscript. LFSS conceived of the study, participated in its design and coordination, and draft the manuscript. All authors read and approved the final manuscript.

Abstract

Background

Melatonin inclusion into in vitro oocyte maturation (IVM) protocols has been suggested because it possesses a powerful free radical scavenger capability that improves the quality of the oocyte used in in vitro embryo production (IVP). The aim of our study was to investigate the presence of melatonin membrane receptors (MT1and MT2) and MT3, which is the melatonin binding site of NQO2 enzyme, in both oocytes and hatched blastocysts to consider an additional subcellular mechanism responsible for the effects of melatonin on IVP.

Methods

The presence of the high affinity melatonin receptors was investigated through an autoradiographic binding assay, using the non-permeable ligand [125I]-iodomelatonin (17 pM) in embryos. The kind of melatonin site was investigated in oocytes and embryos by immunocytochemistry. In vitro fertilized bovine embryos produced from in vitro maturated oocytes supplemented with melatonin (0.0001 to 1000 nM) were analysed to determine their cleavage and blastocyst formation rates.

Results

The [125I]-iodomelatonin (17 pM) binding in blastocysts was blocked by pre-incubation with melatonin (30000 nM), showing the presence of the high affinity melatonin receptors. MT1, MT2 and NQO2 immunoreactivity was observed in oocytes. MT1 immunoreactivity was observed in hatched blastocysts, however MT2 and NQO2 were not observed in this embryonic stage. Melatonin (pM) triggered significant difference in both cleavage and blastocysts formation rates.

Conclusions

The high affinity MT1 melatonin receptor must be taking part in IVM events; furthermore it is the first melatonin receptor to appear during bovine embryo development in vitro.
Zusatzmaterial
Authors’ original file for figure 1
12958_2012_1075_MOESM1_ESM.tiff
Authors’ original file for figure 2
12958_2012_1075_MOESM2_ESM.tiff
Authors’ original file for figure 3
12958_2012_1075_MOESM3_ESM.tiff
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2012

Reproductive Biology and Endocrinology 1/2012 Zur Ausgabe

Neu im Fachgebiet Gynäkologie und Geburtshilfe


 

Meistgelesene Bücher aus dem Fachgebiet

2014 | Buch

Perikonzeptionelle Frauenheilkunde

Fertilitätserhalt, Prävention und Management von Schwangerschaftsrisiken

Ein Praxisbuch für alle, die in der Beratung und Betreuung von Schwangeren und Paaren mit Kinderwunsch tätig sind. Die Herausgeber und Autoren haben alle wichtigen Informationen zusammengetragen, um Frauen sicher zu beraten, zu betreuen und kompetent Verantwortung zu übernehmen.

Herausgeber:
Christian Gnoth, Peter Mallmann

2014 | Buch

Praxisbuch Gynäkologische Onkologie

Das Nachschlagewerk für alle Ärzte in Klinik und Praxis, die Patientinnen mit bösartigen Tumoren des Genitales und der Mamma behandeln. Dieses Buch gibt in kompakter Weise einfach umsetzbare Handlungsanweisungen zum diagnostischen und therapeutischen Vorgehen und Hilfestellungen für die optimale Versorgung der Patientinnen.

Herausgeber:
Edgar Petru, Walter Jonat, Daniel Fink, Ossi R. Köchli

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Gynäkologie und bleiben Sie gut informiert – ganz bequem per eMail.

Bildnachweise