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International Journal of Legal Medicine
Erschienen in:

06.09.2018 | Original Article

Multiplex analysis of genetic polymorphisms within UGT1A9, a gene involved in phase II of Δ9-THC metabolism

verfasst von: Julia Sophie Schneider, Angela Gasse, Marianne Schürenkamp, Ursula Sibbing, Sabrina Banken, Heidi Pfeiffer, Jennifer Schürenkamp, Marielle Vennemann

Erschienen in: International Journal of Legal Medicine | Ausgabe 2/2019

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Abstract

We present a novel multiplex assay for the simultaneous detection of 12 polymorphisms within the UGT1A9 sequence, which codes for enzymes involved in phase II biotransformation. The assay combines a multiplexed amplification step with single-base extension sequencing. The method described here is fast, cost-effective, and easy-to-use, combining the relevant features of screening methods for research and diagnostics in pharmacogenetics. To validate the assay, we tested reproducibility and sensitivity and analysed allele frequencies of 110 Caucasian individuals. Furthermore, we describe combining genetic information of individuals consuming Cannabis sativa products with respective plasma concentrations of a metabolite.
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Metadaten
Titel
Multiplex analysis of genetic polymorphisms within UGT1A9, a gene involved in phase II of Δ9-THC metabolism
verfasst von
Julia Sophie Schneider
Angela Gasse
Marianne Schürenkamp
Ursula Sibbing
Sabrina Banken
Heidi Pfeiffer
Jennifer Schürenkamp
Marielle Vennemann
Publikationsdatum
06.09.2018
Verlag
Springer Berlin Heidelberg
Erschienen in
International Journal of Legal Medicine / Ausgabe 2/2019
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI
https://doi.org/10.1007/s00414-018-1919-0

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