Erschienen in:
06.02.2018 | Brief Report
Mutation of I176R in the E coding region weakens Japanese encephalitis virus neurovirulence, but not its growth rate in BHK-21 cells
verfasst von:
Yuyong Zhou, Rui Wu, Qin Zhao, Yung-Fu Chang, Xintian Wen, Yao Feng, Xiaobo Huang, Yiping Wen, Qigui Yan, Yong Huang, Xiaoping Ma, Xinfeng Han, Sanjie Cao
Erschienen in:
Archives of Virology
|
Ausgabe 5/2018
Einloggen, um Zugang zu erhalten
Abstract
Previously, we isolated the Japanese encephalitis virus (JEV) strain SCYA201201. In this study, we passed the SCYA201201 strain in Syrian baby hamster kidney (BHK-21) cells 120 times to obtain the SCYA201201-0901 strain, which exhibited an attenuated phenotype in mice. Comparison of SCYA201201-0901 amino acid sequences with those of other JEV strains revealed a single mutation, I176R, in the E coding region. Using reverse genetic technology, we provide evidence that this single E-I176R mutation does not affect virus growth in BHK-21 cells but significantly decreases JEV neurovirulence in mice. This study provides critical information for understanding the molecular mechanism of JEV attenuation.