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Journal of Clinical Immunology

Erschienen in:

01.11.2014 | Original Research

Mycobacterium Tuberculosis-Specific Memory NKT Cells in Patients with Tuberculous Pleurisy

verfasst von: Zitao Li, Binyan Yang, Yannan Zhang, Jiangjun Ma, Xinchun Chen, Suihua Lao, Baiqing Li, Changyou Wu

Erschienen in: Journal of Clinical Immunology | Ausgabe 8/2014

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Abstract

Natural killer T (NKT) cells from mouse and human play a protective role in the immune responses against the infection of Mycobacterium tuberculosis. However, the characteristic of CD3⁺TCRvβ11⁺ NKT cells at the local site of M. tuberculosis infection remains poorly defined. In the present study, we found that the numbers of CD3⁺TCRvβ11⁺ NKT cells in pleural fluid mononuclear cells (PFMCs) were significantly lower than those in peripheral blood mononuclear cells (PBMCs). However, CD3⁺TCRvβ11⁺ NKT cells from PFMCs spontaneously expressed high levels of CD69 and CD25 and effector memory phenotypes of CD45RO^highCD62L^lowCCR7^low. After stimulation with the antigens of M. tuberculosis, CD3⁺TCRvβ11⁺ NKT cells from PFMCs produced high levels of IFN-γ. Sorted CD3⁺TCRvβ11⁺ NKT cells from PFMCs cultured with antigen presenting cells (APCs) produced IFN-γ protein and mRNA. The production of IFN-γ could be completely inhibited by AG490 and Wortmannin. In addition, CD3⁺TCRvβ11⁺ NKT cells from PFMCs expressed higher levels of Fas (CD95), FasL (CD178) and perforin but lower levels of granzyme B compared with those from PBMCs. Taken together, our data demonstrated for the first time that M. tuberculosis-specific CD3⁺TCRvβ11⁺ NKT cells participated in the local immune responses against M. tuberculosis through the production of IFN-γ and the secretion of cytolytic molecules.

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Titel: Mycobacterium Tuberculosis-Specific Memory NKT Cells in Patients with Tuberculous Pleurisy
verfasst von: Zitao Li
Binyan Yang
Yannan Zhang
Jiangjun Ma
Xinchun Chen
Suihua Lao
Baiqing Li
Changyou Wu
Publikationsdatum: 01.11.2014
Verlag: Springer US
Erschienen in: Journal of Clinical Immunology / Ausgabe 8/2014
Print ISSN: 0271-9142
Elektronische ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-014-0090-8

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Mycobacterium Tuberculosis-Specific Memory NKT Cells in Patients with Tuberculous Pleurisy

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