Skip to main content
main-content

01.12.2012 | Research | Ausgabe 1/2012 Open Access

Comparative Hepatology 1/2012

N-acetylcysteine improves antitumoural response of Interferon alpha by NF-kB downregulation in liver cancer cells

Zeitschrift:
Comparative Hepatology > Ausgabe 1/2012
Autoren:
Nelson Alexandre Kretzmann, Eduardo Chiela, Ursula Matte, Norma Marroni, Claudio Augusto Marroni
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1476-5926-11-4) contains supplementary material, which is available to authorized users.

Competing interest

The authors declare that they have no competing interests.

Authors’ contributions

NAK made all experiments, data analysis and wrote the paper, EC had worked in cytometry analysis and results discuss, UM gave the laboratory supply and help in on the discussion of results and review the paper, NM gave the financial support and laboratory supply and CAM helped in article writing and revision of data. All Authors read and approved the final manuscript.

Abstract

Background

Liver cancer is one of the most common malignancies in the world and at the moment, there is no drug intervention effective for the treatment of liver tumours. Investigate the effect of N-acetylcysteine (NAC), which has been studied for its antitumoural properties, on the toxicity of hepatocarcinoma (HCC) cells in vitro when used with the drug interferon alpha-2A (IFN), which is used clinically to treat HCC.

Results

NAC, IFN and NAC plus IFN reduced cell viability, as determined by MTT assay. More importantly, NAC potentiates the cytotoxic effect of IFN, with the best response achieved with 10 mM of NAC and 2.5 x 104 of IFN. These results were confirmed by Annexin/PI staining through flow cytometry and morphologic analyses. Co-treatment reduced the expression of the nuclear transcription factor kappa-B (NF-kB). In a similar way to NAC, RNAi against p65 potentiated the toxic effect of IFN, suggesting that, indeed, NAC may be enhancing the effect of IFN through inhibition of NF-kB.

Conclusions

Our results support the notion that NAC may be an important drug for the treatment of liver tumours as primary or adjuvant therapy. IFN has a limited clinical response, and therefore, the anti-proliferative properties of NAC in the liver should be explored further as an alternative for non-responders to IFN treatment.
Zusatzmaterial
Authors’ original file for figure 1
12946_2011_146_MOESM1_ESM.tiff
Authors’ original file for figure 2
12946_2011_146_MOESM2_ESM.tiff
Authors’ original file for figure 3
12946_2011_146_MOESM3_ESM.tiff
Authors’ original file for figure 4
12946_2011_146_MOESM4_ESM.tiff
Authors’ original file for figure 5
12946_2011_146_MOESM5_ESM.tiff
Authors’ original file for figure 6
12946_2011_146_MOESM6_ESM.tiff
Authors’ original file for figure 7
12946_2011_146_MOESM7_ESM.tiff
Authors’ original file for figure 8
12946_2011_146_MOESM8_ESM.tiff
Authors’ original file for figure 9
12946_2011_146_MOESM9_ESM.tiff
Authors’ original file for figure 10
12946_2011_146_MOESM10_ESM.tiff
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2012

Comparative Hepatology 1/2012 Zur Ausgabe

Neu im Fachgebiet Innere Medizin

Meistgelesene Bücher aus der Inneren Medizin

2017 | Buch

Rheumatologie aus der Praxis

Entzündliche Gelenkerkrankungen – mit Fallbeispielen

Dieses Fachbuch macht mit den wichtigsten chronisch entzündlichen Gelenk- und Wirbelsäulenerkrankungen vertraut. Anhand von über 40 instruktiven Fallbeispielen werden anschaulich diagnostisches Vorgehen, therapeutisches Ansprechen und der Verlauf …

Herausgeber:
Rudolf Puchner

2016 | Buch

Ambulant erworbene Pneumonie

Was, wann, warum – Dieses Buch bietet differenzierte Diagnostik und Therapie der ambulant erworbenen Pneumonie zur sofortigen sicheren Anwendung. Entsprechend der neuesten Studien und Leitlinien aller wichtigen Fachgesellschaften.

Herausgeber:
Santiago Ewig

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Innere Medizin und bleiben Sie gut informiert – ganz bequem per eMail.

© Springer Medizin 

Bildnachweise