The online version of this article (https://doi.org/10.1186/s12931-017-0696-5) contains supplementary material, which is available to authorized users.
Nasal gene expression profiling is a promising method to characterize COPD non-invasively. We aimed to identify a nasal gene expression profile to distinguish COPD patients from healthy controls. We investigated whether this COPD-associated gene expression profile in nasal epithelium is comparable with the profile observed in bronchial epithelium.
Genome wide gene expression analysis was performed on nasal epithelial brushes of 31 severe COPD patients and 22 controls, all current smokers, using Affymetrix Human Gene 1.0 ST Arrays. We repeated the gene expression analysis on bronchial epithelial brushes in 2 independent cohorts of mild-to-moderate COPD patients and controls.
In nasal epithelium, 135 genes were significantly differentially expressed between severe COPD patients and controls, 21 being up- and 114 downregulated in COPD (false discovery rate < 0.01). Gene Set Enrichment Analysis (GSEA) showed significant concordant enrichment of COPD-associated nasal and bronchial gene expression in both independent cohorts (FDRGSEA < 0.001).
We identified a nasal gene expression profile that differentiates severe COPD patients from controls. Of interest, part of the nasal gene expression changes in COPD mimics differentially expressed genes in the bronchus. These findings indicate that nasal gene expression profiling is potentially useful as a non-invasive biomarker in COPD.
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- Nasal gene expression differentiates COPD from controls and overlaps bronchial gene expression
Ilse M. Boudewijn
Erica van der Wiel
Eef D. Telenga
Susan J. M. Hoonhorst
Nick H. T. ten Hacken
Huib A. M. Kerstjens
Irene H. Heijink
Marnix R. Jonker
Harold G. de Bruin
J. Sebastiaan Vroegop
Henk R. Pasma
Wim G. Boersma
Frank van den Elshout
Marc E. Lenburg
Dirkje S. Postma
Maarten van den Berge
- BioMed Central
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