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01.12.2017 | Research article | Ausgabe 1/2017 Open Access

BMC Complementary and Alternative Medicine 1/2017

Nelumbo nucifera leaves extracts inhibit mouse airway smooth muscle contraction

Zeitschrift:
BMC Complementary and Alternative Medicine > Ausgabe 1/2017
Autoren:
Xiao Yang, Lu Xue, Qingyang Zhao, Congli Cai, Qing-Hua Liu, Jinhua Shen
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12906-017-1674-7) contains supplementary material, which is available to authorized users.

Abstract

Background

Alkaloids extracted from lotus leaves (AELL) can relax vascular smooth muscle. However, whether AELL has a similar relaxant role on airway smooth muscle (ASM) remains unknown. This study aimed to explore the relaxant property of AELL on ASM and the underlying mechanism.

Methods

Alkaloids were extracted from dried lotus leaves using the high temperature rotary evaporation extraction method. The effects of AELL on mouse ASM tension were studied using force measuring and patch-clamp techniques.

Results

It was found that AELL inhibited the high K+ or acetylcholine chloride (ACh)-induced precontraction of mouse tracheal rings by 64.8 ± 2.9%, or 48.8 ± 4.7%, respectively. The inhibition was statistically significant and performed in a dose-dependent manner. Furthermore, AELL-induced smooth muscle relaxation was partially mediated by blocking voltage-dependent Ca2+ channels (VDCC) and non-selective cation channels (NSCC).

Conclusion

AELL, which plays a relaxant role in ASM, might be a new complementary treatment to treat abnormal contractions of the trachea and asthma.
Zusatzmaterial
Additional file 1: Figure S1. The influence of AELL on ASM. (A) & (B) With AELL washing out, high K+-induced contraction was decreased obviously. (C) & (D) When AELL removed, ACh-induced contraction was decreased obviously, too. (PDF 29 kb)
12906_2017_1674_MOESM1_ESM.pdf
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