nach oben

Erschienen in:

19.10.2018 | Original Research

Nephronectin Expression is Inhibited by Inorganic Phosphate in Osteoblasts

verfasst von: Tadashi Kato, Atsushi Yamada, Kiyohito Sasa, Kentaro Yoshimura, Naoko Morimura, Hiroaki Ogata, Akiko Sakashita, Ryutaro Kamijo

Erschienen in: Calcified Tissue International | Ausgabe 2/2019

Einloggen, um Zugang zu erhalten

Abstract

Nephronectin (Npnt), an extracellular matrix protein, is known to be a ligand of integrin α8β1, and it has also been known to play critical roles as various organs. In the present study, elevated extracellular inorganic phosphate (Pi) strongly inhibited the expression of Npnt in MC3T3-E1 cells, while the existence of extracellular calcium (Ca) was indispensable for its effect. Furthermore, Pi-induced inhibition of Npnt gene expression was recovered by inhibitors of both sodium-dependent Pi transporter (Pit) and fibroblast growth factor receptors (Fgfrs). These results demonstrated that Npnt gene expression is regulated by extracellular Pi via Pit and Fgfrs.

Nur mit Berechtigung zugänglich

Morimura N, Tezuka Y, Watanabe N, Yasuda M, Miyatani S, Hozumi N, Tezuka Ki K (2001) Molecular cloning of POEM: a novel adhesion molecule that interacts with alpha8beta1 integrin. J Biol Chem 276:42172–42181CrossRefPubMed

Kahai S, Lee SC, Seth A, Yang BB (2010) Nephronectin promotes osteoblast differentiation via the epidermal growth factor-like repeats. FEBS Lett 584:233–238CrossRefPubMed

Brandenberger R, Schmidt A, Linton J, Wang D, Backus C, Denda S, Müller U, Reichardt LF (2001) Identification and characterization of a novel extracellular matrix protein nephronectin that is associated with integrin α8β1 in the embryonic kidney. J Cell Biol 154:447–458CrossRefPubMedPubMedCentral

Linton JM, Martin GR, Reichardt LF (2007) The ECM protein nephronectin promotes kidney development via integrin alpha8beta1-mediated stimulation of Gdnf expression. Development 134:2501–2509CrossRefPubMedPubMedCentral

Miyazono A, Yamada A, Morimura N, Takami M, Suzuki D, Kobayashi M, Tezuka K, Yamamoto M, Kamijo R (2007) TGF-beta suppresses POEM expression through ERK1/2 and JNK in osteoblasts. FEBS Lett 581:5321–5326CrossRefPubMed

Tsukasaki M, Yamada A, Suzuki D, Aizawa R, Miyazono A, Miyamoto Y, Suzawa T, Takami M, Yoshimura K, Morimura N, Yamamoto M, Kamijo R (2011) Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-alpha. Biochem Biophys Res Commun 410:766–770CrossRefPubMed

Kurosawa T, Yamada A, Takami M, Suzuki D, Saito Y, Hiranuma K, Enomoto T, Morimura N, Yamamoto M, Iijima T, Shirota T, Itabe H, Kamijo R (2015) Expression of nephronectin is inhibited by oncostatin M via both JAK/STAT and MAPK pathways. FEBS Open Bio 5:303–307CrossRefPubMedPubMedCentral

Iezumi Y, Yamada A, Minami E, Ikehata M, Yoshida Y, Kato T, Morimura N, Ogata H, Sakashita A, Iijima T, Chikazu D, Kamijo R (2017) IL-1beta suppresses nephronectin expression in osteoblasts via ERK1/2 and JNK. Biochem Biophys Res Commun 493:773–775CrossRef

Michigami T (2013) Extracellular phosphate as a signaling molecule. Contrib Nephrol 180:14–24CrossRef

10.

Beck GR Jr, Moran E, Knecht N (2003) Inorganic phosphate regulates multiple genes during osteoblast differentiation, including Nrf2. Exp Cell Res 288:288–300CrossRefPubMed

11.

Kimata M, Michigami T, Tachikawa K, Okada T, Koshimizu T, Yamazaki M, Kogo M, Ozono K (2010) Signaling of extracellular inorganic phosphate up-regulates cyclin D1 expression in proliferating chondrocytes via the Na+/Pi cotransporter Pit-1 and Raf/MEK/ERK pathway. Bone 47:938–947CrossRefPubMed

12.

Nishino J, Yamazaki M, Kawai M, Tachikawa K, Yamamoto K, Miyagawa K, Kogo M, Ozono K, Michigami T (2017) Extracellular phosphate induces the expression of dentin matrix protein 1 through the FGF receptor in osteoblasts. J Cell Biochem 118:1151–1163CrossRefPubMed

13.

Bergwitz C, Juppner H (2011) Phosphate sensing. Adv Chronic Kidney Dis 18:132–144CrossRefPubMedPubMedCentral

14.

Ganesh SK, Stack AG, Levin NW, Hulbert-Shearon T, Port FK (2001) Association of elevated serum PO(4), Ca x PO(4) product, and parathyroid hormone with cardiac mortality risk in chronic hemodialysis patients. J Am Soc Nephrol 12:2131–2138PubMed

15.

Kalantar-Zadeh K, Kuwae N, Regidor DL, Kovesdy CP, Kilpatrick RD, Shinaberger CS, McAllister CJ, Budoff MJ, Salusky IB, Kopple JD (2006) Survival predictability of time-varying indicators of bone disease in maintenance hemodialysis patients. Kidney Int 70:771–780CrossRefPubMed

16.

Katsumata K, Kusano K, Hirata M, Tsunemi K, Nagano N, Burke SK, Fukushima N (2003) Sevelamer hydrochloride prevents ectopic calcification and renal osteodystrophy in chronic renal failure rats. Kidney Int 64:441–450CrossRefPubMed

17.

Arai-Nunota N, Mizobuchi M, Ogata H, Yamazaki-Nakazawa A, Kumata C, Kondo F, Hosaka N, Koiwa F, Kinugasa E, Shibata T, Akizawa T (2014) Intravenous phosphate loading increases fibroblast growth factor 23 in uremic rats. PLoS ONE 9:e91096CrossRefPubMedPubMedCentral

18.

Wolf M (2010) Forging forward with 10 burning questions on FGF23 in kidney disease. J Am Soc Nephrol 21:1427–1435CrossRefPubMed

19.

Hiranuma K, Yamada A, Kurosawa T, Aizawa R, Suzuki D, Saito Y, Nagahama R, Ikehata M, Tsukasaki M, Morimura N, Chikazu D, Maki K, Shirota T, Takami M, Yamamoto M, Iijima T, Kamijo R (2016) Expression of nephronectin is enhanced by 1alpha,25-dihydroxyvitamin D3. FEBS Open Bio 6:914–918CrossRefPubMedPubMedCentral

20.

Kato T, Yamada A, Ikehata M, Yoshida Y, Sasa K, Morimura N, Sakashita A, Iijima T, Chikazu D, Ogata H, Kamijo R (2018) FGF-2 suppresses expression of nephronectin via JNK and PI3K pathways. FEBS Open Bio 8:836–842CrossRefPubMedPubMedCentral

21.

Jung YK, Han MS, Park HR, Lee EJ, Jang JA, Kim GW, Lee SY, Moon D, Han S (2018) Calcium-phosphate complex increased during subchondral bone remodeling affects earlystage osteoarthritis. Sci Rep 8:487CrossRefPubMedPubMedCentral

22.

Collins JF, Bai L, Ghishan FK (2004) The SLC20 family of proteins: dual functions as sodium-phosphate cotransporters and viral receptors. Pflugers Arch 447:647–652CrossRefPubMed

23.

Loghman-Adham M (1996) Use of phosphonocarboxylic acids as inhibitors of sodium-phosphate cotransport. Gen Pharmacol 27:305–312CrossRefPubMed

24.

Foster BL, Nociti FH Jr, Swanson EC, Matsa-Dunn D, Berry JE, Cupp CJ, Zhang P, Somerman MJ (2006) Regulation of cementoblast gene expression by inorganic phosphate in vitro. Calcif Tissue Int 78:103–112CrossRefPubMed

25.

Sugita A, Kawai S, Hayashibara T, Amano A, Ooshima T, Michigami T, Yoshikawa H, Yoneda T (2011) Cellular ATP synthesis mediated by type III sodium-dependent phosphate transporter Pit-1 is critical to chondrogenesis. J Biol Chem 286:3094–3103CrossRefPubMed

26.

Durcan L, Bolster F, Kavanagh EC, McCarthy GM (2014) The structural consequences of calcium crystal deposition. Rheum Dis Clin North Am 40:311–328CrossRefPubMed

27.

Nasi S, So A, Combes C, Daudon M, Busso N (2016) Interleukin-6 and chondrocyte mineralisation act in tandem to promote experimental osteoarthritis. Ann Rheum Dis 75:1372–1379CrossRefPubMed

28.

Kawai M, Kinoshita S, Ozono K, Michigami T (2016) Inorganic phosphate activates the AKT/mTORC1 pathway and shortens the life span of an alphaKlotho-deficient model. J Am Soc Nephrol 27:2810–2824CrossRef

29.

Okamoto T, Taguchi M, Osaki T, Fukumoto S, Fujita T (2014) Phosphate enhances reactive oxygen species production and suppresses osteoblastic differentiation. J Bone Miner Metab 32:393–399CrossRefPubMed

Titel: Nephronectin Expression is Inhibited by Inorganic Phosphate in Osteoblasts
verfasst von: Tadashi Kato
Atsushi Yamada
Kiyohito Sasa
Kentaro Yoshimura
Naoko Morimura
Hiroaki Ogata
Akiko Sakashita
Ryutaro Kamijo
Publikationsdatum: 19.10.2018
Verlag: Springer US
Erschienen in: Calcified Tissue International / Ausgabe 2/2019
Print ISSN: 0171-967X
Elektronische ISSN: 1432-0827
DOI: https://doi.org/10.1007/s00223-018-0484-3

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

23.04.2024 | Ablationstherapie | Nachrichten

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Nephronectin Expression is Inhibited by Inorganic Phosphate in Osteoblasts

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Wie erfolgreich ist eine Re-Ablation nach Rezidiv?

Europäische Ernährungsgewohnheiten: Das sind die häufigsten Fehler

Hinter dieser Appendizitis steckte ein Erreger

Demenzkranke durch Antipsychotika vielfach gefährdet

Update Innere Medizin

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2019

Bone Microstructure in Response to Vitamin D3 Supplementation: A Randomized Placebo-Controlled Trial

Lack of Association Between Vitamin D and Hand Grip Strength in Asians: A Nationwide Population-Based Study

Efficacy of rhBMP-2 in Cleft Lip and Palate Defects: Systematic Review and Meta-analysis

Reduction of Hip and Other Fractures in Patients Receiving Teriparatide in Real-World Clinical Practice: Integrated Analysis of Four Prospective Observational Studies

Pre-treatment with Pamidronate Improves Bone Mechanical Properties in Mdx Mice Treated with Glucocorticoids

Health Care Costs Associated With Muscle Weakness: A UK Population-Based Estimate

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Wie erfolgreich ist eine Re-Ablation nach Rezidiv?

Europäische Ernährungsgewohnheiten: Das sind die häufigsten Fehler

Hinter dieser Appendizitis steckte ein Erreger

Demenzkranke durch Antipsychotika vielfach gefährdet

Update Innere Medizin