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01.12.2017 | Research article | Ausgabe 1/2017 Open Access

BMC Complementary and Alternative Medicine 1/2017

Neuroprotective effect of Aronia melanocarpa extract against glutamate-induced oxidative stress in HT22 cells

BMC Complementary and Alternative Medicine > Ausgabe 1/2017
Hyeon Yong Lee, Jin Bae Weon, Gahee Ryu, Woo Seung Yang, Nam Young Kim, Myong Ki Kim, Choong Je Ma



Glutamate (an endogenous excitatory neurotransmitter) at high concentrations contributes to the development of neurodegenerative diseases. Aronia melanocarpa (A. melanocarpa) berries contain anthocyanins and have high antioxidant activities. In this study, we evaluated whether A. melanocarpa berries could protect neuronal cells against glutamate-induced oxidative stress.


A. melanocarpa berries exerted a protective effect against cytotoxicity in HT22 mouse hippocampal cells by MTT assay. We evaluated oxidative stress parameters including ROS level, intracellular Ca2+ level, glutathione level and antioxidant enzyme activity in HT22 cells to elucidate the mechanism of its neuroprotective effect.


A. melanocarpa berries decreased glutamate-induced death of HT22 cells. In addition, A. melanocarpa berries reduced ROS and intracellular Ca2+ levels. Glutathione level, antioxidant enzymes, glutathione reductase and glutathione peroxide activities and mitochondrial membrane potential were also increased in HT22 cells.


These results suggested that A. melanocarpa berries protected HT22 cells by exerting an antioxidant effect.
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