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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

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11.01.2022 | Original article

New bone-generative effect of Salvia officinalis L. in the expanded midpalatal suture

An in vivo and in vitro study

verfasst von: Dr. Emre Kayalar, PhD, DDS, MPharm, Dr. Fatih Goger, PhD, MPharm, Dr. Gul Tas Deynek, PhD, DDS, Dr. Olgu Enis Tok, PhD, Prof. Dr. Sevim Kucuk, PhD, MPharm

Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Sonderheft 1/2022

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Abstract

Purpose

The aims of this study were to evaluate the effects of systemic administration of Salvia officinalis Linnaeus (L.) leaf extract on new bone formation in the expanded premaxillary suture in rats in vivo and to examine the antioxidant effects and phenolic profile of Salvia officinalis (SO) leaf and root extracts in vitro.

Methods

Fourteen male Sprague Dawley rats were allocated to two groups: SO group (n = 7) and control group (n = 7). An open-loop spring was attached to the upper incisors of each rat to expand the premaxillae. A 5-day expansion period followed by a 12-day retention period was observed. The rats in the SO group received systemic administration of 20 mg SO/kg/day via the orogastric route for 17 days. Histomorphometric examinations were carried out to examine the amount of new bone formation, number of capillaries, and intensity of inflammatory cell response. Immunohistochemical analysis was conducted to examine the number of osteoblasts and osteoclasts. Leaf and root extracts of SO were also analyzed for antioxidant activity and phenolic compounds in vitro.

Results

Statistical analysis showed that the following were higher in the SO group than in the control group: new bone formation, number of osteoblasts and osteoclasts, intensity of inflammatory cell response (neutrophils, lymphocytes, and macrophages), and number of capillaries. The major compound identified in SO leaf extract was rosmarinic acid, while luteolin derivatives, salvianolic acid F, and medioresinol were also present.

Conclusions

Salvia officinalis L. from leaf extract provided antioxidant effects and stimulated enhanced new bone formation in the expanded midpalatal suture after maxillary expansion in rats.

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Titel: New bone-generative effect of Salvia officinalis L. in the expanded midpalatal suture
An in vivo and in vitro study
verfasst von: Dr. Emre Kayalar, PhD, DDS, MPharm
Dr. Fatih Goger, PhD, MPharm
Dr. Gul Tas Deynek, PhD, DDS
Dr. Olgu Enis Tok, PhD
Prof. Dr. Sevim Kucuk, PhD, MPharm
Publikationsdatum: 11.01.2022
Verlag: Springer Medizin
Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Ausgabe Sonderheft 1/2022
Print ISSN: 1434-5293
Elektronische ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-021-00366-3

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New bone-generative effect of Salvia officinalis L. in the expanded midpalatal suture

An in vivo and in vitro study

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

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Conclusions

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