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01.12.2014 | Brief communication | Ausgabe 1/2014 Open Access

Journal of Ovarian Research 1/2014

New construction of an animal model for the orthotopic transplantation of an ovarian tumor

Zeitschrift:
Journal of Ovarian Research > Ausgabe 1/2014
Autoren:
Hui Zhang, Xinping Gao, Yongan Yang, Weiming Wang, Jin Liu, Yijuan Liang, Hongli Wu, Jinjin Qin, Kun Pan, Yifeng Wang, Junrong Shi, Youju Ma
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1757-2215-7-64) contains supplementary material, which is available to authorized users.

Competing interests

We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript. All authors have participated sufficiently in this work to take public responsibility for it. All authors have reviewed the final version of the manuscript and approved it for publication. Neither this manuscript nor one with substantially similar content under my (our) authorship has been published or is being considered for publication elsewhere; this manuscript has been submitted with the full knowledge and approval of the institution or organization given as the affiliation of the author.

Authors’ contribution

HZ is responsible for the experimental operation and writing papers; XG is responsible for feeding the mice; YY is responsible for recording the relevant experimental data do statistical analysis; WW, JL, YH, JK are responsible for pathological examination, fluorescence microscopy, YW is responsible for financial support. In addition, JS and YM are responsible for experimental guidance. SJS (Email: 18903363120@163.com) is added to author 11 and YM (Email: 18903363120@163.com) is added to author 12. All authors read and approved the final manuscript.

Abstract

A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was assessed using flow cytometry. The inoculated site was hand palpated and the signs and symptoms related to tumor growth were observed with the naked eye. The allophycocyanin (APC) indirectly labeled mouse-antihuman CA125 and fluorescein isothiocyanate (FITC)-labeled anti-mouse MHC Class I molecule (H-2Kd/H-2Dd) were observed using a confocal laser scanning microscope. The animal model of ovarian cancer constructed using this method can more directly reflect the characteristics of cancer cells. It provides reliable experimental results and presents a technical platform for the research of ovarian cancer stem cells.
Zusatzmaterial
Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 3
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Authors’ original file for figure 5
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Authors’ original file for figure 6
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Literatur
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