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09.01.2017 | Original Article

NMR-based metabolomics reveals brain region-specific metabolic alterations in streptozotocin-induced diabetic rats with cognitive dysfunction

verfasst von: Hong Zheng, Qiuting Lin, Dan Wang, Pengtao Xu, Liangcai Zhao, Wenyi Hu, Guanghui Bai, Zhihan Yan, Hongchang Gao

Erschienen in: Metabolic Brain Disease | Ausgabe 2/2017

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Abstract

Diabetes mellitus (DM) can result in cognitive dysfunction, but its potential metabolic mechanisms remain unclear. In the present study, we analyzed the metabolite profiling in eight different brain regions of the normal rats and the streptozotocin (STZ)-induced diabetic rats accompanied by cognitive dysfunction using a ¹H NMR-based metabolomic approach. A mixed linear model analysis was performed to assess the effects of DM, brain region and their interaction on metabolic changes. We found that different brain regions in rats displayed significant metabolic differences. In addition, the hippocampus was more susceptible to DM compared with other brain regions in rats. More interestingly, significant interaction effects of DM and brain region were observed on alanine, creatine/creatine-phosphate, lactate, succinate, aspartate, glutamate, glutamine, γ-aminobutyric acid, glycine, choline, N-acetylaspartate, myo-inositol and taurine. Based on metabolic pathway analysis, we speculate that cognitive dysfunction in the STZ-induced diabetic rats may be associated with brain region-specific metabolic alterations involving energy metabolism, neurotransmitters, membrane metabolism and osmoregulation.

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Titel: NMR-based metabolomics reveals brain region-specific metabolic alterations in streptozotocin-induced diabetic rats with cognitive dysfunction
verfasst von: Hong Zheng
Qiuting Lin
Dan Wang
Pengtao Xu
Liangcai Zhao
Wenyi Hu
Guanghui Bai
Zhihan Yan
Hongchang Gao
Publikationsdatum: 09.01.2017
Verlag: Springer US
Erschienen in: Metabolic Brain Disease / Ausgabe 2/2017
Print ISSN: 0885-7490
Elektronische ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-016-9949-0

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NMR-based metabolomics reveals brain region-specific metabolic alterations in streptozotocin-induced diabetic rats with cognitive dysfunction

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