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01.01.2007

Novel Analysis of Clonal Diversification in Blood B Cell and Bone Marrow Plasma Cell Clones in Immunoglobulin Light Chain Amyloidosis

verfasst von: ROSHINI S. ABRAHAM, MICHELLE K. MANSKE, NETA S. ZUCKERMAN, ABHISHEK SOHNI, HANNA EDELMAN, GITIT SHAHAF, MICHAEL M. TIMM, ANGELA DISPENZIERI, MORIE A. GERTZ, RAMIT MEHR

Erschienen in: Journal of Clinical Immunology | Ausgabe 1/2007

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Abstract

Immunoglobulin light chain amyloidosis (AL) is characterized by a limited clonal expansion of plasma cells and amyloid formation. Here, we report restriction in the diversity of VL gene usage with a dominance of clonally related B cells in the peripheral blood (PB) isotype-specific repertoire of AL patients. A rigorous quantification of lineage trees reveals presence of intraclonal variations in the PB clones compared to the bone marrow (BM) clones, which suggests a common precursor that is still subject to somatic mutation. When compared to normal BM and PB B cells, AL clones showed significant but incomplete impairment of antigenic selection, which could not be detected by conventional R and S mutation analysis. Therefore, graphical analysis of B cell lineage trees and mathematical quantification of tree properties provide novel insights into the process of B cell clonal evolution in AL.

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Titel: Novel Analysis of Clonal Diversification in Blood B Cell and Bone Marrow Plasma Cell Clones in Immunoglobulin Light Chain Amyloidosis
verfasst von: ROSHINI S. ABRAHAM
MICHELLE K. MANSKE
NETA S. ZUCKERMAN
ABHISHEK SOHNI
HANNA EDELMAN
GITIT SHAHAF
MICHAEL M. TIMM
ANGELA DISPENZIERI
MORIE A. GERTZ
RAMIT MEHR
Publikationsdatum: 01.01.2007
Verlag: Kluwer Academic Publishers-Plenum Publishers
Erschienen in: Journal of Clinical Immunology / Ausgabe 1/2007
Print ISSN: 0271-9142
Elektronische ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-006-9056-9

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Novel Analysis of Clonal Diversification in Blood B Cell and Bone Marrow Plasma Cell Clones in Immunoglobulin Light Chain Amyloidosis

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