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12.02.2018 | Original Article

Novel evaluation method of dentin repair by direct pulp capping using high-resolution micro-computed tomography

verfasst von: Motoki Okamoto, Yusuke Takahashi, Shungo Komichi, Manahil Ali, Naomichi Yoneda, Takuya Ishimoto, Takayoshi Nakano, Mikako Hayashi

Erschienen in: Clinical Oral Investigations | Ausgabe 8/2018

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Abstract

Objectives

We evaluated a novel micro-computed tomography (micro-CT) assessment for quality and quantity of dentin repair, which is difficult to visualize by histological analysis, after direct pulp capping under standardized cavity preparation.

Materials and methods

Standardized cavities were prepared on Wistar rats and direct pulp capping was performed using two commercial bioceramics, ProRoot MTA, and iRoot BP Plus. After 2 or 4 weeks, quality and quantity of tertiary dentin formation were evaluated using high-resolution micro-CT analyses including dentin mineral density, dentin mineral contents, compactness and integrity of tertiary dentin, and dentin volume with/without void space. Reproducibility of micro-CT analyses was confirmed by histological evaluation of the same specimen.

Results

The exposed pulp area sizes were similar between iRoot BP Plus and ProRoot MTA. Micro-CT analysis of 2-week samples showing compactness of tertiary dentin was significantly higher in iRoot BP Plus than ProRoot MTA (p < 0.05). Tertiary dentin volume without void space, dentin mineral contents, and density were not significantly different between the groups. In 4-week samples, a significant increase was observed in dentin mineral density, compactness, and dentin volume with/without void space induced by iRoot BP Plus (p < 0.05). Micro-CT analysis of tertiary dentin integrity demonstrated that some ProRoot MTA specimens had small defects and lacked continuity (6/512 images). No defects were observed with iRoot BP Plus.

Conclusions

Micro-CT analysis was confirmed as an accurate, objective, and inclusive approach for evaluating quality and quantity of dentin repair.

Clinical relevance

These multifaceted approaches to evaluate pulp capping materials may accelerate review processes, ultimately improving vital pulp therapy.

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Titel: Novel evaluation method of dentin repair by direct pulp capping using high-resolution micro-computed tomography
verfasst von: Motoki Okamoto
Yusuke Takahashi
Shungo Komichi
Manahil Ali
Naomichi Yoneda
Takuya Ishimoto
Takayoshi Nakano
Mikako Hayashi
Publikationsdatum: 12.02.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 8/2018
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2374-5

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Novel evaluation method of dentin repair by direct pulp capping using high-resolution micro-computed tomography

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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