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Inflammation Research

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04.09.2022 | Original Research Paper

Novel insight into ferroptosis-related genes, molecular subtypes, and immune characteristics in intracranial aneurysms

verfasst von: Shifu Li, Qian Zhang, Zhou Chen, Zheng Huang, Longbo Zhang, Fenghua Chen

Erschienen in: Inflammation Research | Ausgabe 10-11/2022

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Abstract

Objectives

This study aimed to identify the role of ferroptosis in intracranial aneurysm (IA).

Methods

GSE122897, GSE75436, GSE15629, and GSE75434 datasets were downloaded from the Gene Expression Omnibus database. The differentially expressed ferroptosis-related genes (DEFRGs) were selected to construct a diagnostic model integrating with machine learning. Then, a consensus clustering algorithm was performed to classify IA patients into distinct ferroptosis-related clusters. Functional analyses, including GO, KEGG, GSVA, and GSEA analyses, were conducted to elucidate the underlying mechanisms. ssGSEA and xCell algorithms were performed to uncover the immune characteristics.

Results

We identified 28 DEFRGs between IAs and controls from the GSE122897 dataset. GO and KEGG results showed that these genes were enriched in cytokine activity, ferroptosis, and the IL-17 signaling pathway. Immune analysis showed that the IAs had higher levels of immune infiltration. A four FRGs model (MT3, CDKN1A, ZEP69B, and ABCC1) was established and validated with great IA diagnostic ability. We divided the IA samples into two clusters and found that cluster 2 had a higher proportion of rupture and immune infiltration. We identified 10 ferroptosis phenotypes-related markers in IAs.

Conclusion

Ferroptosis and the immune microenvironment are closely associated with IAs, providing a basis for understanding the IA development.

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Titel: Novel insight into ferroptosis-related genes, molecular subtypes, and immune characteristics in intracranial aneurysms
verfasst von: Shifu Li
Qian Zhang
Zhou Chen
Zheng Huang
Longbo Zhang
Fenghua Chen
Publikationsdatum: 04.09.2022
Verlag: Springer International Publishing
Erschienen in: Inflammation Research / Ausgabe 10-11/2022
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-022-01633-8

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Novel insight into ferroptosis-related genes, molecular subtypes, and immune characteristics in intracranial aneurysms

Abstract

Objectives

Methods

Results

Conclusion

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

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Abstract

Objectives

Methods

Results

Conclusion

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