Background
Methods
Materials
Patient cohort
Immunohistochemistry
Cell culture
siRNA transfection
Preparation of cellular extract and immunoblotting
WST-1 proliferation assay
Cell death detection ELISA Plus
Wound healing assay
Statistical analysis
Results
Localisation-dependant associations between disease specific survival and SFK pathway
Univariate survival analysisb | Multivariate survival analysisc | ||||
---|---|---|---|---|---|
Disease specific survival | Disease specific survival | ||||
Clinico-pathological characterisitcs | Patient (n (%)) | Hazard ratio (95 % CIa) | p-value | Hazard ratio (95 % CIa) | p-value |
Age (≤61/>61) | 94 (49)/98 (51) | 0.97 (0.50–1.86) | 0.916 | ||
T-stage (I/II/III/IV) | 83 (43)/33 (17)/69 (36)/7 (4) | 2.13 (1.46–3.12) |
9.4×10
−5
| ||
Grade (I/II/III/IV) | 15 (8)/59 (30)/84 (44)/34 (18) | 2.16 (1.40–3.35) |
0.001
| 1.56 (1.02–2.40) |
0.042
|
Recurrence (None/recurred) | 142 (74)/50 (26) | 11.47 (5.22–25.20) |
6.78×10
−15
| 12.50 (5.09–30.64) |
1.0×10
−7
|
KLINTRUP (Low/High) | 96 (50)/96 (50) | 0.97 (0.50–1.87) | 0.932 |
Protein | Median (IQRa) | p-value |
---|---|---|
Src Kinase | ||
Membrane | 50 (27–80) | 0.237 |
Cytoplasmic | 37 (27–68) | 0.132 |
Nucleus | 2 (0–12) | 0.726 |
SFK (Y416) | ||
Membrane | 5 (0–13) | 0.206 |
Cytoplasmic | 7 (0–20) | 0.868 |
Nucleus | 2 (0–11) | 0.735 |
FAK (Y861) | ||
Membrane | 13 (3–37) |
0.008
|
Cytoplasmic | 7 (0–20) | 0.546 |
Nuclear | 60 (43–77) | 0.532 |
FAK (Y397) | ||
Membrane | 93 (41–147) |
0.001
|
Cytoplasmic | 77 (33–133) | 0.252 |
Nuclear | 152 (127–180) | 0.846 |
Paxillin | ||
Membrane | 3 (0–10) |
0.050
|
Cytoplasmic | 7 (0–10) | 0.335 |
Nuclear | 3 (0–63) |
0.031
|
FYN | ||
Membrane | 15 (2–30) |
0.006
|
Cytoplasmic | 8 (2–17) | 0.393 |
Nuclear | 13 (0–109) | 0.139 |
LYN | ||
Membrane | 37 (10–77) | 0.141 |
Cytoplasmic | 70 (38–202) | 0.446 |
Nuclear | 13 (0–109) |
0.019
|
FGR | ||
Membrane | 7 (0–22) | 0.087 |
Cytoplasmic | 33 (15–60) | 0.252 |
Nuclear | 9 (3–27) | 0.503 |
HCK | ||
Membrane | 63 (27–103) |
0.038
|
Cytoplasmic | 47 (30–27) | 0.109 |
Nuclear | 57 (30–80) | 0.197 |
YES | ||
Membrane | 47 (17–70) |
0.0004
|
Cytoplasm | 38 (23–69) | 0.271 |
Nucleus | 43 (27–62) | 0.842 |