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Clinical Oral Investigations

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26.01.2019 | Original Article

Nuclear morphological changes in gingival epithelial cells of patients with periodontitis

verfasst von: Antonija Tadin, Lidia Gavic, Marija Roguljic, Daniel Jerkovic, Davor Zeljezic

Erschienen in: Clinical Oral Investigations | Ausgabe 10/2019

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Abstract

Objectives

This study assessed the frequency of nuclear morphological changes in gingival epithelial cells, as a biomarker for DNA damage, in individuals with periodontitis, before and after implementation of periodontal therapy, and compared the morphology to those with healthy periodontal tissues.

Materials and methods

Exfoliated gingival cells were taken from 30 participants without periodontal destruction in any teeth and 30 participants with periodontitis before and after 45 and 90 days following treatment. Nuclear morphological changes were analyzed using the micronucleus test.

Results

Compared with the healthy volunteers, those with periodontitis had a significant increase in the number of cells with nuclear broken eggs (P = 0.048), condensed chromatin (P = 0.015), karyolysis (P < 0.001), or binuclei (P < 0.001). In the periodontitis group, the pretreatment frequencies of cells with micronuclei (P = 0.008), binuclei (P < 0.001), karyolysis (P = 0.038), nuclear buds (P = 0.005), and condensed chromatin (P = 0.015) were significantly higher than 90 days after treatment.

Conclusion

Our observations suggest that periodontal disease increases the frequency of nuclear morphological changes in gingival epithelial cells and that the implementation of periodontal therapy was associated with a reduction of that number.

Clinical relevance

The micronucleus test could serve as a tool for estimating genotoxic damage in assessing the success of periodontal therapy.

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Titel: Nuclear morphological changes in gingival epithelial cells of patients with periodontitis
verfasst von: Antonija Tadin
Lidia Gavic
Marija Roguljic
Daniel Jerkovic
Davor Zeljezic
Publikationsdatum: 26.01.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 10/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-02803-5

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Nuclear morphological changes in gingival epithelial cells of patients with periodontitis

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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Abstract

Objectives

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Results

Conclusion

Clinical relevance

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