Background
Methods
Subjects
Measurements
Genome wide methylation chip
Pyrosequencing
Statistical analysis
Results
Cases | Controls | |
---|---|---|
N | 7 | 7 |
Age, years | 15.8 ± 1.0 (14.5 to 17.2) | 15.9 ± 1.4 (15.1 to 18.1) |
*BMI, kg/m2
| 39.0 ± 1.7 (37.2 to 41.2) | 17.0 ± 0.7 (16.4 to 17.8) |
*BMI percentile | 0.996 ± 0.001 (0.995 to 0.997) | 0.048 ± 0.022 (0.026 to 0.091) |
SBP, mmHg | 125.8 ± 13.9 (115.3 to 152.7) | 112.7 ± 8.9 (100.7 to 124.7) |
†‡Fasting insulin, uU/ml | 35.9 ± 13.6 (19.8 to 58.9) | 13.7 ± 3.0 (9.4 to 17.0) |
‡Fasting glucose, mg/dl | 103.5 ± 14.8 (84 to 126) | 88.8 ± 7.6 (80 to 100) |
*‡QUICKI | 0.28 ± 0.01 (0.27 to 0.30) | 0.33 ± 0.01 (0.31 to 0.35) |
Gene | More significant CpG site | Second CpG site | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
ID | Dis. to TSS | Average Case/Con. (%) | Difference (%) |
P
| FDR | ID | Dis. to TSS | Average Case/Con. (%) | Difference (%) |
P
| |
UBASH3A
| 13517 | -156 | 45.6/35.5 | 10.1 | 5.00 × 10-6
| 0.14 | 146 | 52 | 45.6/37.7 | 7.9 | 0.145 |
CTNND1 | 6160 | -431 | 57.4/70.2 | -12.8 | 2.00 × 10-5
| 0.20 | 18016 | 415 | 19.7/21.5 | -1.8 | 0.353 |
TRIM3
| 17029 | -348 | 6.74/9.28 | -2.54 | 2.40 × 10-5
| 0.20 | 19207 | -331 | 12.5/16.2 | -3.7 | 0.035 |
CTSZ*
| 23241 | -292 | 13.6/21.0 | -7.40 | 4.40 × 10-5
| 0.24 | 1644 | -592 | 17.4/25.6 | -8.2 | 0.001 |
TRAF5 | 10097 | -319 | 69.7/58.0 | 11.7 | 4.50 × 10-5
| 0.24 | 25786 | 77 | 1.90/1.79 | 0.1 | 0.738 |
HIPK3
| 25599 | 390 | 51.5/64.9 | -13.4 | 6.50 × 10-5
| 0.26 | 5514 | 314 | 26.6/35.0 | -8.4 | 0.006 |
DPCR1 | 20918 | -46 | 62.5/70.2 | -7.70 | 6.90 × 10-5
| 0.26 | 4703 | -906 | 72.4/73.0 | -0.6 | 0.709 |
HIF3A | 2879 | 153 | 5.35/2.97 | 2.38 | 1.08 × 10-4
| 0.34 | 7009 | -1445 | 57.1/63.0 | -5.9 | 0.091 |
NOTCH4 | 5969 | -51 | 59.1/68.5 | -9.40 | 1.15 × 10-4
| 0.34 | 14700 | 4 | 19.3/23.6 | -4.3 | 0.078 |
CDH5
| 8829 | -243 | 27.2/36.5 | -9.20 | 1.75 × 10-4
| 0.44 | 22286 | 36 | 72.5/81.3 | -8.8 | 0.012 |
CREB3L3
| 23739 | 42 | 9.63/36.7 | -27.1 | 1.78 × 10-3
| 0.59 | 13445 | -26 | 5.74/25.8 | -20.1 | 0.004 |
Cases | Controls | |
---|---|---|
N | 45 | 46 |
*Age, years | 20.3 ± 5.0 (14.1 to 29.5) | 17.6 ± 3.1 (12.7 to 27.9) |
†BMI, kg/m2
| 37.4 ± 6.1 (30.5 to 59.9) | 18.9 ± 1.2 (16.4 to 22.0) |
†SBP, mmHg | 125.8 ± 14.2 (97 to 175) | 113.5 ± 8.5 (100 to 134) |
Gene | ID | Dis. to TSS | Average Case/Con. (%) | Difference (%) |
P
| Age adjusted
P
|
---|---|---|---|---|---|---|
UBASH3A |
13517
|
-156
|
45.1/41.8
|
3.30
|
0.0019
|
0.008
|
TRIM3 | --- | -357 | 8.58/8.98 | -0.40 | 0.3128 | 0.059 |
17029
|
-348
|
8.76/9.97
|
-1.21
|
0.0004
|
0.001
| |
19207 | -331 | 6.91/7.93 | -1.02 | 0.0050 | 0.022 | |
CTSZ |
23241
|
-292
|
16.5/17.7
|
-1.20
|
0.0953
|
0.271
|
--- | -279 | 27.8/29.1 | -1.30 | 0.8425 | 0.798 | |
HIPK3 | --- | 375 | 48.0/55.3 | -7.30 | 0.0184 | 0.061 |
25599
|
390
|
49.8/57.1
|
-7.30
|
0.0135
|
0.050
| |
--- | 422 | 39.8/45.8 | -6.00 | 0.0164 | 0.070 | |
--- | 438 | 42.5/49.0 | -6.50 | 0.0173 | 0.061 | |
CDH5 |
8829
|
-243
|
30.3/33.4
|
-3.10
|
0.0450
|
0.092
|
CREB3L3 | --- | 30 | 3.23/2.85 | 0.38 | 0.7188 | 0.828 |
23739
|
42
|
18.7/21.6
|
-2.90
|
0.3251
|
0.288
| |
--- | 48 | 18.5/22.3 | -3.80 | 0.1549 | 0.174 |
Biological process | GO. ID | Term |
P-value | Adjust P
|
---|---|---|---|---|
Level 3 | GO:0006955 | Immune response | 0.017 | 0.690 |
Level 3 | GO:0007588 | Excretion | 0.028 | 0.690 |
Level 3 | GO:0001775 | Cell activation | 0.042 | 0.690 |
Level 3 | GO:0001816 | Cytokine production | 0.044 | 0.690 |
Level 3 | GO:0009607 | Response to biotic stimulus | 0.047 | 0.690 |
Molecular function | GO. ID | Term |
P value | Adjust P
|
Level 3 | GO:0019842 | Vitamin binding | 0.009 | 0.522 |
Level 3 | GO:0003823 | Antigen binding | 0.010 | 0.522 |
Level 3 | GO:0004872 | Receptor activity | 0.032 | 0.756 |
Level 3 | GO:0008289 | Lipid binding | 0.035 | 0.756 |