Introduction
Obestatin (OB) is a newly discovered peptide encoded by the ghrelin gene together with acylated (AG) and unacylated ghrelin (UAG), produced in the oxyntic mucosa of the stomach [
1‐
3]. It has been initially suggested that OB behaves as a physiological opponent to ghrelin, through interaction with the orphan G-protein-coupled receptor 39 (GPR39) [
4]. However, these findings have been questioned lately and a number of studies have failed to confirm the anorexigenic effect of obestatin [
5‐
7] and its agonist properties on GPR39 [
8,
9]. Therefore, to date, some biological actions of OB seem to be controversial and its specific receptor remains unknown. Recent data suggest a relevant role of peptides encoded by the ghrelin gene in glucose balance and their implications for diabetes. Although the direct protective effects exerted by ghrelin gene-derived peptides on β-cells and pancreatic islets has been demonstrated in streptozotocin (STZ)-treated neonatal rats [
10‐
13], to our knowledge, limited data exist regarding their ability to protect the myocardial tissue in adult diabetic animals, in which the ability to induce β-cell regeneration is lost.
OB plays a significant role in cardiac function in humans, both under physiological and pathological conditions. In particular, higher saliva OB levels were found in overweight patients with ischemic heart disease compared with healthy controls [
14], whereas they are significantly decreased in serum of subjects with type 2 diabetes mellitus [
1] and obesity [
15]. Increased OB levels and altered ghrelin to OB ratio are present in chronic heart failure (CHF) patients with cachexia [
16], as well in spontaneously hypertensive rats [
17]. OB improves myocardial function and reduces cell death and apoptosis of cardiomyocytes after ischemia/reperfusion (I/R) in isolated rat heart [
18]. These effects are probably mediated by OB receptors present on cardiac cells and by activation of signaling pathways included in the reperfusion injury salvage kinases (RISK), such as phosphoinositide 3-kinase (PI3K), protein kinase C (PKC) and extracellular signal regulated kinases (ERK) 1/2 [
18]. In type 1 diabetes, hyperglycemia is the active element leading to cardiac dysfunctions. Hyperglycemia promotes the production of reactive oxygen species (ROS) and contributes to cardiac fibrosis and contractile dysfunctions [
19‐
23]. The protective effects exerted by OB against myocardial dysfunctions in ischemic-reperfused heart led us to investigate whether OB may rescue myocardial contractility and beta adrenergic response in the heart of STZ treated diabetic rats. The main results obtained in this study suggest that OB displayed a beneficial effect against the reduction of contractility and β-adrenergic response, as well of α-myosin heavy chain (MHC) and β-1 adrenoreceptors expression in the heart of STZ-treated diabetic rats, and that the protective effect is probably related to the ability of OB to restore oxidative balance and to promote phosphorylation/modulation of AMPK and pro-survival kinases such as Akt, ERK1/2 and glycogen synthase kinase (GSK) 3β.
Materials and methods
Cell culture
H9c2 cardiomyoblasts, an embryonic rat heart-derived cell line, were obtained from the Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia (Brescia, Italy) and cultured in 25 cm2 flasks with Dulbecco’s Modified Eagle Medium (DMEM) containing 10% foetal calf serum (FCS) at 37°C in a humidified atmosphere with 95% O2 and 5% CO2. Cells were starved for 24 hours in DMEM with 1% FCS and then treated with 20 mM metformin (Sigma-Aldrich, Milan, Italy), as positive control for AMPK phosphorylation, or 50 nM OB (rat obestatin (1–23), produced by conventional solid-phase synthesis and purified (97%) by reverse-phase HPLC by NeoMPS, Strasbourg, France). H9c2 were then scraped, centrifugated (1500 rpm, 5 minutes, 4°C) and lysed for total protein extraction.
Animals
Male Wistar rats (Harlan-Laboratories, Udine, Italy) weighing 200–220 g were provided with Piccioni pellet diet (no. 48, Gessate Milanese, Italy) and water ad libitum. The animals were cared for in compliance with the Local Ethical Committee and Italian Ministry of Health Guidelines (no. 86/609/EEC) and with the Principles of Laboratory Animal Care (NIH no. 85–23, revised 1985). The scientific project was supervised and approved by the Italian Ministry of Health, Rome, and by the ethical committee of the University of Torino.
Animals acute treatment
The effects of an acute treatment with OB or metformin were tested in a group of fifteen rats. Five rats received a daily i.p. injection of metformin (250 mg/kg/day) for three consecutive days, as indicated by Zhou
et al.[
24]. A second group of five rats received a single dose of OB by i.p. injection 1 hour before killing, following the same procedures described by Kola
et al.[
25] for ghrelin treatment. Five control rats received intraperitoneal injections of saline. All rats were anesthetized with 90 mg /kg of Zoletil 100 i.p. (Virbac, Carros, France) and killed by aortic exsanguinations. Hearts were removed and left ventricle was excised and rapidly homogenized to obtain protein extracts for western blotting analyses.
Animals chronic treatment
In a group of rats basal glucose level was measured by saphenous vein puncture with the Accu-Check Compact kit (Roche Diagnostics Gmbh, Mannheim, Germany). Hyperglycemia was induced in a group of rats through a single injection of streptozotocin (STZ) (50 mg/kg b.wt) in the tail vein (diabetic, DM). A group of rats received only the vehicle (non-diabetic, ND). Three days later glucose was measured and only rats with blood glucose levels above 200 mg/dL entered the experimental protocols. ND and DM rats were distributed into four groups: untreated ND (n = 18) (ND-CTRL), obestatin-treated ND (n = 10) (ND-OB), untreated DM (n = 20) (DM-CTRL); obestatin-treated DM (n = 18) (DM-OB). OB was administered subcutaneously twice a day for 6 weeks, at the final dose of 25 μg/kg/5 ml.
Two days before the sacrifice, after a fasting period of 12 h, a 50% glucose solution at the dose of 3 g/kg was orally administered to the rats. Glucose levels were tested 0, 15, 30, 45, 60, 90, 120 and 240 min after glucose loading by saphenous vein puncture.
Rats were sacrificed after 6 weeks. All rats were anesthetized with Zoletil 100 i.p. and killed by aortic exsanguinations. Blood was collected and the plasma isolated and placed at −80°C. Glucose level was immediately measured. The heart was isolated, weighed and portions of left ventricle were immediately homogenized to obtain different tissue extracts. To evaluate papillary muscles contractility and β-adrenergic response, papillary muscles were isolated from left ventricle, as described below.
Plasma detection
Triglyceride (TG), total cholesterol (TC), high-density-lipoprotein (HDL) and low density-lipoprotein (LDL) were determined in plasma by standard enzymatic procedures using reagents kits (Hospitex Diagnostics, Florence, Italy). Plasma insulin was measured by an ultrasensitive insulin enzyme-linked immunosorbent assay kit from DRG Diagnostics (Marburg, Germany). The content of TNF-α was determined by using a rat TNF-α ELISA kit (Diaclone, Besancon, France) following the manufacturer’s guidelines. Each sample was tested in duplicate and averaged. Final results were calculated from the kit standards and expressed as picograms of TNF-α per milliliter.
For cytosolic and nuclear proteins extraction, left ventricles were homogenized at 10% (w/v) using a Potter Elvehjem homogenizer (Wheaton, NJ, USA) in a homogenization buffer [20 mM HEPES, pH 7.9, 1 mM MgCl2, 0.5 mM EDTA, 1 mM EGTA, 1 mM dithiothreitol (DTT), 0.5 mM phenylmethylsulphonyl fluoride (PMSF), 5 μg/ml aprotinin, and 2.5 μg/ml leupeptin]. Homogenates were cleared by centrifugation at 1,000 g for 5 min at 4°C. Supernatants were removed and centrifuged at 15,000 g at 4°C for 40 min to obtain the cytosolic fraction. The pelleted nuclei were resuspended in an extraction buffer [20 mM HEPES, pH 7.9, 1.5 mM MgCl2, 0.2 mM EDTA, 20% (w/v) glycerol, 1 mM EGTA, 1 mM DTT, 0.5 mM PMSF, 5 μg/ml aprotinin, and 2.5 μg/ml leupeptin]. The suspensions were incubated on ice for 30 min for high-salt extraction followed by centrifugation at 15,000 g for 20 min at 4°C. The resulting supernatants containing nuclear proteins were carefully removed and stored.
Total extracts were obtained both from 10% (w/v) left heart ventricle homogenates and cell lysates in RIPA buffer [0.5% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 10 mmol/l EDTA, and protease inhibitors]. After 40 minutes of incubation in ice, samples were cleared by centrifugation at 15,000 g at 4°C for 40 min. Supernatants were removed and stored. Protein content was determined using the Bradford assay. Protein extracts were stored at −80°C until use.
Oxidative biochemical parameters
The levels of reactive oxygen species (ROS) were measured on total extracts using the probe 2’,7’-dichlorofluorescin diacetate (DCFH-DA) and measured fluorimetrically [
26]. Reduced and oxidized glutathione content was evaluated in cytosolic fractions following Owens’s method [
27]. The difference between total glutathione and reduced GSH content represents the GSSG content (expressed as μg/mg prot.); the ratio between GSSG content and GSH is considered a good parameter of antioxidant status. Hydroxynonenal (HNE) concentration was also determined on cytosol fractions. After extraction, an aliquot of cytosol was injected into an HPLC (Waters Associated, Milford, MA, USA) Symmetry C
18 column (5 mm, 3.9x150 mm). The mobile phase was acetonitrile:bidistilled water (42%, v/v). The HNE concentration was calculated by comparison with a standard solution of HNE of known concentration.
Western blot
NFkB-p65 was detected on cytosol and on nuclear extracts; AMPK, pAMPK, Akt, pAkt, ERK1/2, pERK1/2, pGSK3β, β1-adrenoreceptor and β-actin were detected on total extracts. Equal amounts of proteins (60 μg) were separated on 7.5% SDS-polyacrylamide gels, then blotted on nitrocellulose membranes (Amersham Biosciences, Braunschweig, Germany). The membranes were incubated overnight with primary antibodies and reacted with appropriated peroxidase-labeled secondary antibodies (Biorad). Immunoreactive proteins were detected through the chemiluminescence assay (ECL, Amersham) and subsequent exposure to film.
Specific bands were quantified by densitometry using an analytic software (Bio-Rad, Multi-Analyst, Munchen, Germany) and the net intensity of phosphorylated proteins was normalized for the intensity of the corresponding total protein. β-actin was used as internal loading control for pGSK3β and cytosolic NFkB, while lamin A was used for nuclear NFkB.
Semiquantitative RT-PCR
Total RNA was extracted from left ventricle using TriPure Isolation Reagent (Roche Diagnostics). Gene transcripts for α- and β-MHC, β1-adrenoreceptor, CTGF, TGFβ1 and GADPH were quantified by PCR. All experiments were performed on at least three independent cDNA preparations. PCR products were electrophoresed on 2% agarose gels and amplification products were stained with GelStar nucleic acid gel stain (FMC BioProducts, Rockland, ME, USA). Gels were photographed and analyzed with Kodak 1D Image Analysis software. The primer sequences were: α-MHC forward 5’ AGCCTCTCATCTCGCATCTC 3’; reverse 5’ GGACCACCCATC TCACTTT 3’, β-MHC forward 5’ ACCGCTGAGACAGAGAATGG 3’; reverse 5’ GGGTTGGCTTGGATGATTT 3’, β1-adrenoreceptor forward 5’ GCCGATCTGG TCATGGGA 3’; reverse 5’ GTTGTAGCAGCGGCGCG 3’, CTGF forward 5’ CAAGGACCG CACGTGGTT 3’; reverse 5’ CCCTAGCTGCCTACCGACTGGAAGACAC 3’, TGFβ1 forward 5’ CTGCTGGCAA TAGCTTC CTA – 3’; reverse 5’ CGAGCCTTAGTTTGGACAGGAT 3’; GAPDH forward 5’ AGATCCACAACGGATACATT 3’; reverse 5’ TCCCTCAAGATTGTCAGCAA 3’.
Papillary muscle and contractility determination
Papillary muscles isolated from the left ventricle were superfused with oxygenated (100% O2) Tyrode solution containing (in mM): 154 NaCl, 4 KCl, 2 CaCl2,1 MgCl2, 5.5 glucose, 5 N-(2-hydroxyethyl)-piperazineN’-ethanesufonic acid (HEPES); pH 7.4 adjusted with NaOH) warmed to 37°C, and driven at constant frequency (120 beats/min). Isometric twitches were evaluated by a 60–2997 Harvard transducer, visualized on a Tektronix 2211 digital storage oscilloscope and acquired and recorded in a Power Mac computer, using the Labview Software (National Instruments Corp., Texas, USA). To study the responsiveness to β-adrenergic stimulation, after 20–30 min equilibration, papillary muscles obtained from the different groups of animals were treated with isoproterenol (Iso, 1 μM). Preliminary studies showed that 1 μM was the minimal saturating concentration of Iso.
Statistical analysis
All values were expressed as means ± SD, and were analyzed by one-way analysis of variance (ANOVA) followed by Bonferroni’s multiple comparison test or by Newman-Keuls multiple range test, when appropriate. P < 0.05 was considered statistically significant.
Discussion
The major new findings in this study are that: 1) OB displayed a beneficial effect against the alterations of contractility and β-adrenergic response in the heart of STZ-treated diabetic rats; 2) the protective effect is related to the ability of OB to restore oxidative balance and to promote phosphorylation/modulation of AMPK and pro-survival kinases such as Akt, ERK1/2 and GSK3β. To our knowledge, this is the first report showing that OB is able to exert a protective action in myocardial tissue affected by experimentally induced diabetes in adult animals.
OB is a newly discovered peptide, which derives from the ghrelin peptide precursor pre-pro-ghrelin [
1,
2,
4]. Although it has been suggested that both OB and ghrelin participate in a complex regulatory system [
32], the intracellular pathways activated by OB and the role it plays in physiological and in pathophysiological conditions are largely unknown. Several studies have already indicated that, in common with ghrelin and other growth hormone secretagogues, OB protects cardiac muscle against I/R injury [
33]. Moreover, it has been shown that ghrelin reduces oxidative stress and inhibits the production of reactive oxygen species [
34,
35], biological events that have been implicated in various pathologies, including hypertension [
36], cardiac ischemia [
37] and myocardial fibrosis [
19,
20,
23,
29]. A similar protective effect has been also reported in primary cardiomyocytes, as well as in H9c2 cardiomyoblastic cell line and endothelial cells, in which both ghrelin [
21] and OB [
18] inhibit apoptosis induced by doxorubicin or I/R, by activating ERK1/2, PKC and PI3K/Akt dependent mechanisms. Several evidences indicate that ghrelin gene-derived peptides prevent the development of diabetes in STZ-treated newborn rats. Indeed, both ghrelin and OB promote regeneration of β-cells and improve glucose metabolism in STZ-treated newborn rats, granting a therapeutic potential in medical conditions associated with impaired β-cell function [
10‐
13]. Moreover, a recent paper showed that ghrelin ameliorated the reductions in motor and sensory nerve conduction velocities and reduced oxidative stress in STZ-treated mice [
38], suggesting that ghrelin gene-derived peptides could also be able to protect against dysfunction in experimentally-induced diabetes in adult animals, in which it is likely these peptides have lost their ability to induce β-cell regeneration. In addition, ghrelin improves both the metabolic functions and the disturbed energy metabolism in the cardiac muscle of obese diabetic rats [
39].
Among all diabetic complications, cardiovascular disease continues to be the primary cause of morbidity and mortality. In agreement with other studies [
16,
40], we previously reported that diabetic cardiomyopathy induced by chronic hyperglycemia is characterized by myocyte loss and myocardial fibrosis, which lead to decreased elasticity and impaired contractile function [
29]. Moreover, the enhanced oxidative stress reduced peak contractile amplitude and maximal velocity of contraction and relaxation under basal condition, as well as the β-adrenergic response in diabetic myocardial tissue [
29,
30].
This study demonstrates that OB exerts a protective effect against the derangement of contractility observed in papillary muscles from diabetic rats (−65% of mechanical tension
vs control). The beneficial effect of OB could be due to its ability to counteract the switch of cardiac myosin heavy chain gene expression from the α- to the β-MHC isoform, and the increase of pro-fibrogenic growth factors, such as TGFβ1 and CTGF, observed in diabetic myocardial tissue. In addition, OB was also able to restore the β-adrenergic response by promoting recovery of β1-adrenoreceptors protein expression. The molecular mechanisms leading to myocardial dysfunction observed in diabetic myocardial tissue include an unbalance between the pro-oxidant and antioxidant compounds and increased inflammatory process, in terms of TNF-α plasma levels and NFkB activation. We observed that OB corrects oxidative unbalance and reduces inflammatory processes in diabetic myocardium, although, on the basis of our data, we do not provide a clear evidence that the initial mechanism upon which OB acts is related to a direct antioxidative action. We thus investigated the possible molecular mechanisms and intracellular pathway involved in the protective effect of OB. OB enhanced AMPK phosphorylation and up-regulated pro-survival kinases Akt, ERK1/2 and GSK3β in diabetic myocardial tissue. The ability of OB to enhance pAMPK levels in myocardial cells was confirmed by in both in vivo and in vitro models after acute administration of this peptide. Interestingly, the effects of OB were comparable to those induced by metformin, an anti-diabetic drug known to stimulate AMPK. AMPK has recently emerged as an important intracellular signaling pathway in the heart [
31]. In the heart, AMPK is modulated by hormones such as adiponectin, leptin and ghrelin, or cytokines like TNFα. A recent paper by Paiva [
41], showing that the administration of metformin during the early reperfusion significantly enhances AMPK phosphorylation, concomitantly with the reduction of infarct size, highlighted the important role of AMPK activation in cardioprotective mechanisms. It has been proposed that AMPK may protect against reperfusion injury by increasing glucose uptake, if the potential negative consequences of increased fatty acid oxidation are not present [
42]. Since ATP from glycolysis may be preferentially used to support membrane activities such as ion pumping, a shift in glucose metabolism may play an important role in cardiac contractile function, metabolic activity and calcium homeostasis under conditions of calcium overload, such as post-ischemic recovery [
43] or during β-adrenergic stimulation [
44].
In addition, AMPK activation has been involved in the reduction of inflammatory markers such as TNFα and NFkB transcription factor [
45]. Together with Akt, AMPK is considered the main signaling molecule controlling cardiac functions [
46]. The observed decrease of cardiac AMPK phosphorylation may therefore contribute, together with reduced Akt signaling, to the depressed myocardial contractility observed in diabetic rats. Indeed, it has been recently reported that AMPK activation may induce an enhancement of L-type calcium current I(Ca) and a prolongation of the action potential duration in cardiomyocytes [
47].
Besides AMPK, OB induced phosphorylation of Akt, ERK1/2 and GSK3β, pro-survival kinases known to exert a protective role against oxidative stress [
20,
23,
48], and proposed as integral components of a protective cascade involved in myocardial preconditioning against I/R [
49,
50]. The involvement of these kinases in the action of OB has been already shown in the proliferation of human retinal pigment epithelial and gastric cancer cells [
51,
52], and in rodent β-cells and human pancreatic islets cell survival [
10,
11].
Oxidative stress can trigger the opening of the mitochondrial membrane permeability transition pore (mPTP) and lead to a significant loss of mitochondrial NAD
+ stores and subsequent derangement of cellular energy reserves and intact cellular functions. Mitochondrial dysfunction plays an important role in the development of diabetes and insulin resistance, and proper cellular function during diabetes requires the maintenance of mitochondrial membrane potential [
23]. The present study shows that OB also promoted phosphorylation/inactivation of GSK3β. GSK3β is a substrate of multiple pro-survival protein kinases, including Akt and ERK1/2, and is therefore a step to which multiple protective signaling pathways converge [
53]. Since phosphorylated GSK3β limits the opening of mPTP, it is reasonable to think that GSK3β inactivation plays a crucial role in the protective effect afforded by OB in diabetic myocardial tissue.
We cannot exclude, however, that the protective effect of OB may result from additional mechanisms not investigated in the present study, such as the nitric oxide (NO) pathway activation. Recent results, indeed, indicate that increased NO availability attenuates some alterations in metabolism and gene expression associated with insulin resistance induced by a high fat diet [
54].
A still open question regards the earliest receptor-initiated mechanisms involved in the action of OB. Although OB was initially claimed to activate the G protein-coupled receptor-39 (GPR39) [
4], subsequent studies were unable to demonstrate binding of OB to GPR39 or a stimulatory function of the OB peptide on GPR39-transfected cells [
8,
9], and the original proposal that OB acts as a ligand of GPR39 has been retracted [
28]. We have recently provided evidence that specific OB receptors are present in ventricular myocardial cells [
18], with a receptor density and binding affinity quite close to those previously found in rodent and human pancreatic β-cells, where OB promotes cell survival and induces expression of genes involved in the regulation of β-cell mass and function [
10,
11]. In conclusion, although to date the precise role of OB in cardiovascular pathophysiology remains still partially unknown, the observed beneficial effect in diabetic myocardial tissue confirms the relevance of this peptide as a physiological agent exerting protective effects against cardiac dysfunction and oxidative stress, already shown in the case of ischemic/reperfused heart.
Competing interests
No conflict of interest, financial or otherwise, are declared by the authors.
Authors’ contribution
Authors’ contribution: experiments planning and manuscript drafting: MA, GA and GM; cell culture and animal treatment: CG, EA and RM; cardiac contractility studies: EB and GA; biochemical parameters detection, western blot and PCR: MA and RM. All author read and approved the final manuscript.