Suk Ji and Joo-Cheol Park contributed equally to this work.
Odontogenic Ameloblast-Associated Protein (ODAM) in gingival crevicular fluid (GCF) can provide evidence of the detachment of junctional epithelium from the tooth surface by periodontitis. This study sought to investigate the ability of ODAM to reflect the severity of periodontitis at a site-specific level; thus whether there was a relationship between clinical diagnostic parameters and the value of ODAM in GCF was analyzed.
Eight periodontitis patients with various severities were enrolled, and the clinical parameters and samples of GCF were obtained from 44 to 60 sites of each subject. The ODAM concentration was quantified by enzyme-linked immunosorbent assay. Correlation analyses between clinical parameters and ODAM values and unadjusted and adjusted (linear) mixed model analyses were performed. The accuracy of ODAM to reflect sites having a probing depth (PD) ≥ 5 mm and a positive bleeding on probing (BOP) was evaluated by receiver-operating characteristic analysis.
A total of 424 GCF samples were collected. The mean ODAM concentration from each patient varied from 0.2 to 1.52 ng/ml. Correlations between PD or clinical attachment level (CAL) and ODAM values were found (p < 0.0001). An adjusted linear mixed model showed that PD or CAL were associated with ODAM values (p < 0.05). The area under the curve of ODAM, which reflected sites with PD ≥ 5 mm and positive BOP, was 0.661 (p < 0.0001).
This result shows the possibility of GCF ODAM as a site-specific biomarker for periodontal tissue destruction.
Lee HK, Ji S, Park SJ, Choung HW, Choi Y, Lee HJ, Park SY, Park JC. Odontogenic Ameloblast-associated protein (ODAM) mediates junctional epithelium attachment to teeth via integrin-ODAM-rho guanine nucleotide exchange factor 5 (ARHGEF5)-RhoA signaling. J Biol Chem. 2015;290:14740–53. CrossRefPubMedPubMedCentral
Salvi GE, Berglundh T, Lang NP. Examination of patients. In: Lang NP, Lindhe J, editors. Clinical periodontology and implant dentistry. 6th ed. Oxford: Munksgaard; 2015. p. 559–73.
AlRowis R, AlMoharib HS, AlMubarak A, Bhaskardoss J, Preethanath RS, Anil S. Oral fluid-based biomarkers in periodontal disease - part 2. Gingival crevicular fluid J Int Oral Health. 2014;6:126–35. PubMed
Kendall M, Gibbons JD. Rank correlation methods. 5th ed. New York: Oxford University Press; 1990.
Mineoka T, Awano S, Rikimaru T, Kurata H, Yoshida A, Ansai T, Takehara T. Site-specific development of periodontal disease is associated with increased levels of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in subgingival plaque. J Periodontol. 2008;79:670–6. CrossRefPubMed
Nakayama Y, Kobayashi R, Matsui S, Matsumura H, Iwai Y, Noda K, Yamazaki M, Kurita-Ochiai T, Yoshimura A, Shinomura T, Ganss B, Ogata Y. Localization and expression pattern of amelotin, odontogenic ameloblast-associated protein and follicular dendritic cell-secreted protein in the junctional epithelium of inflamed gingiva. Odontology. 2017;105:329–37. CrossRefPubMed
Lang NP, Tonetti MS. Periodontal risk assessment (PRA) for patients in supportive periodontal therapy (SPT). Oral health Prev Dent. 2003;1:7–16. PubMed
- Odontogenic ameloblast-associated protein (ODAM) in gingival crevicular fluid for site-specific diagnostic value of periodontitis: a pilot study
Soo Jin Kim
Young Ho Kim
- BioMed Central
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