Introduction
Materials and methods
Serum free bovine granulosa cell culture
RNA isolation, cDNA synthesis and real- time PCR
Name | Sequence | Size (bp) | NCBI accession no. |
---|---|---|---|
ESR2 Forward | GGTCAATCCATCCTACCCCT | 264 | NM_174051.3 |
ESR2 Reverse | TTCACGCCAAGGACTCTTTT | ||
FOXL2 Forward | AGCCAAGTTCCCGTTCTACG | 140 | NM_001031750.1 |
FOXL2 Reverse | GGTCCAGCGTCCAGTAGTTG | ||
FST Forward | GCACTGGCCGCCTGAGCACCT | 191 | NM_175801 |
FST Reverse | TGGGGCACAGACGCAGCGGG | ||
PPARG forward | TATCCCCGGCTTTGTGAACC | 288 | NM_181024.2 |
PPARG Reverse | GGGCGGTCTCCACTGAGAAT | ||
PTGS2 Forward | TACAGCACTTGAGTGGCTATCAC | 317 | NM_174445 |
PTGS2 Reverse | CTGGTCAATTGAAGCCTTTGATAC | ||
SOX9 Forward | ACCTGGAACTTCAGTGGCG | 147 | XM_010816647.1 |
SOX9 Reverse | CCAAGTAGGGGAAGGCGAAT | ||
TBP Forward | GCCTTGTGCTTACCCACCAACAGTTC | 200 | NM_001075742.1 |
TBP Reverse | TGTCTTCCTGAAACCCTTCAGAATAGGG |