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01.12.2014 | Original Article | Ausgabe 4/2014

Journal of Artificial Organs 4/2014

Optimal method for short-term or long-term islet preservation: comparison of islet culture, cold preservation and cryopreservation

Zeitschrift:
Journal of Artificial Organs > Ausgabe 4/2014
Autoren:
Fei Liu, Wencong Tian, Yinan Yang, Qiong Zhang, Mengmeng Zhu, Liang Yang, Lei Yang, Jing Li, Jie Liu, Ping Wu, Kaichiang Yang, Ximo Wang, Yanna Shen, Zhi Qi

Abstract

Islet preservation plays an important role for the success of islet transplantation. To determine the optimal method for islet preservation, we compared the outcomes of islet culture, cold preservation, and cryopreservation in this study. Isolated rat islets were divided into three groups: 37 °C group (conventional culture at 37 °C in RPMI-1640 medium), 4 °C group (cold preservation at 4 °C in University of Wisconsin (UW) solution), and −80 °C group (cryopreservation at −80 °C with dimethyl sulfoxide (DMSO)). Recovery rate, Calcein-AM/PI double staining, insulin release, mRNA level of hypoxia-inducible factor-1α (HIF-1α), and protein level of Bax in islets were examined after short-term (1 day) or long-term (7 days) preservation. After either short-term or long-term preservation, 4 °C group showed higher recovery rate of the islets number, lower percentage of PI positive area, better insulin release ability, and lower expression levels of HIF-1α and Bax in comparison to the 37 or −80 °C group. Meanwhile, islets in 37 °C group showed better function, and down-regulation of HIF-1α and Bax than those in −80 °C group on day 1; however, worse function of islets, up-regulated HIF-1α and Bax in 37 °C group were observed in comparison to −80 °C group on day 7. These results suggest that cold preservation at 4 °C in UW solution is the optimal method in comparison to the conventional culture at 37 °C or cryopreservation at −80 °C for short-term or long-term islet preservation. Furthermore, the potential mechanism may relate to, at least in part, apoptosis induced by the HIF-1α.

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