Optimization of mouse oocyte vitrification through microencapsulation in sodium alginate hydrogel
- 21.09.2025
- Gamete Biology
Erschienen in:
- Verfasst von
- Jing Shen
- Na Ye
- Yuqi Zhang
- Chenxi Liu
- Weijie Li
- Xinli Zhou
- Erschienen in
- Journal of Assisted Reproduction and Genetics | Ausgabe 10/2025
Abstract
Purpose
Vitrification (ice-free) is considered more effective than slow freezing for oocyte cryopreservation. However, high concentrations of toxic cryoprotective agents (CPAs) are typically required, followed by multi-step washing to remove CPAs, risking osmotic shock and impairing oocytes development. This study aimed to establish a hydrogel encapsulation strategy using a microfluidic system to improve vitrification outcomes.
Methods
Three different sizes of oocyte-loaded sodium alginate hydrogel microspheres (OHMs) (~ 262 µm, ~ 193 µm, and ~ 156 µm) were prepared using an optimized three-channel microfluidic system. The effects of particle size, vitrification solutions (VS1, VS2, VS3, and VS4), CPA loading durations (4, 8, and 12 min), and warming/dilution procedures (one-step, two-step, and three-step) on oocyte vitrification were systematically evaluated.
Results
OHMs with a particle size of 262 µm, loaded with VS3 (containing 10% DMSO) for 8 min and subjected to the two-step warming and dilution procedure, achieved excellent post-thaw survival (91.98%), cleavage (75.84%), and blastocyst rates (23.86%). The survival rate was comparable to that of the Cryotop group vitrified with 15% DMSO (91.81%), while the cleavage and blastocyst rates were significantly higher than those of the Cryotop group (67.42% and 20.03%, respectively). Besides, OHMs resulted in minimal oocyte volume changes upon CPA loading (minimum normalized volume of 0.8739) compared to the Cryotop multi-step method (0.4396).
Conclusions
Compared with the Cryotop carrier method, the microfluidic hydrogel microcapsule technique reduces CPA concentration, shortens loading time, and minimizes osmotic injury. This approach provides a promising strategy for fertility preservation and may be extended to other biological samples.
Graphical Abstract
Anzeige
- Titel
- Optimization of mouse oocyte vitrification through microencapsulation in sodium alginate hydrogel
- Verfasst von
-
Jing Shen
Na Ye
Yuqi Zhang
Chenxi Liu
Weijie Li
Xinli Zhou
- Publikationsdatum
- 21.09.2025
- Verlag
- Springer US
- Erschienen in
-
Journal of Assisted Reproduction and Genetics / Ausgabe 10/2025
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330 - DOI
- https://doi.org/10.1007/s10815-025-03673-5
Dieser Inhalt ist nur sichtbar, wenn du eingeloggt bist und die entsprechende Berechtigung hast.
