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Erschienen in: Journal of Parasitic Diseases 3/2016

20.09.2014 | Original Article

Optimization of murine model for Besnoitia caprae

verfasst von: A. Oryan, R. Sadoughifar, M. Namavari

Erschienen in: Journal of Parasitic Diseases | Ausgabe 3/2016

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Abstract

It has been shown that mice, particularly the BALB/c ones, are susceptible to infection by some of the apicomplexan parasites. To compare the susceptibility of the inbred BALB/c, outbred BALB/c and C57 BL/6 to Besnoitia caprae inoculation and to determine LD50, 30 male inbred BALB/c, 30 outbred BALB/c and 30 C57 BL/6 mice were assigned into 18 groups of 5 mice. Each group was inoculated intraperitoneally with 12.5 × 103, 25 × 103, 5 × 104, 1 × 105, 2 × 105 tachyzoites and a control inoculum of DMEM, respectively. The inbred BALB/c was found the most susceptible strain among the experienced mice strains so the LD50 per inbred BALB/c mouse was calculated as 12.5 × 103.6 tachyzoites while the LD50 for the outbred BALB/c and C57 BL/6 was 25 × 103.4 and 5 × 104 tachyzoites per mouse, respectively. To investigate the impact of different routes of inoculation in the most susceptible mice strain, another seventy five male inbred BALB/c mice were inoculated with 2 × 105 tachyzoites of B. caprae via various inoculation routes including: subcutaneous, intramuscular, intraperitoneal, infraorbital and oral. All the mice in the oral and infraorbital groups survived for 60 days, whereas the IM group showed quicker death and more severe pathologic lesions, which was then followed by SC and IP groups. Therefore, BALB/c mouse is a proper laboratory model and IM inoculation is an ideal method in besnoitiosis induction and a candidate in treatment, prevention and testing the efficacy of vaccines for besnoitiosis.
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Metadaten
Titel
Optimization of murine model for Besnoitia caprae
verfasst von
A. Oryan
R. Sadoughifar
M. Namavari
Publikationsdatum
20.09.2014
Verlag
Springer India
Erschienen in
Journal of Parasitic Diseases / Ausgabe 3/2016
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-014-0563-y

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