The authors declare that they have no competing interests.
All authors contributed to the data collection and manuscript preparation. AMS, LG, AMG, NM and RS contributed to the cell culture and other in vitro assays. AMS, NM and RS also participated in all statistical analyses and interpretation of the data. All authors approved the final version of the manuscript.
Caffeine is an active alkaloid that can cause damage to bones in formation during prenatal life into adulthood. This compound can pass across the placenta and into the mother’s milk, causing a reduction in bone formation, growth and mass. The objective of this study was to examine the osteogenic potential of osteoblasts extracted from neonatal rats born to mothers treated with caffeine throughout pregnancy.
Twenty-four adult Wistar rats were randomly divided into four groups, consisting of one control group and three groups that were treated with 25, 50, or 100 mg/kg of caffeine by an oral-gastric probe throughout the duration of the experimental period (pregnancy). At birth, three puppies from each dam in each group were euthanized, and osteoblasts were extracted from the calvaria of these pups for in vitro testing.
The osteoblasts extracted from the pups of rats that received 50 mg/kg caffeine during pregnancy exhibited increased expression of osteocalcin, osteopontin, sialoprotein, runx-2, alkaline phosphatase and type I collagen transcripts, resulting in increased synthesis of mineralization nodules.
Neonates from rats treated with 50 mg/kg caffeine during pregnancy contained osteoblasts with a higher osteogenic potential characterized by increased expression of osteocalcin, osteopontin, sialoprotein, runx-2, alkaline phosphatase and type I collagen and increased synthesis of mineralization nodules.
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- Osteogenic potential of osteoblasts from neonatal rats born to mothers treated with caffeine throughout pregnancy
Amanda Maria Sena Reis
Lorena Gabriela Rocha Ribeiro
Natália de Melo Ocarino
Alfredo Miranda Goes
- BioMed Central
Neu im Fachgebiet Orthopädie und Unfallchirurgie
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