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01.12.2012 | Primary research | Ausgabe 1/2012 Open Access

Cancer Cell International 1/2012

Osteopontin’s colocalization with the adhesion molecule CEACAM5 in cytoplasm of carcinoma of tongue and its correlation with the invasion of that diease

Cancer Cell International > Ausgabe 1/2012
Fan Zhang, Xu Jin Liu, Xun Qu, Zhen Sheng Hu, Yong Mei Yang, Ling Ma, Pei Liu, Ping Shi, Feng Cai Wei
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2867-12-33) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

FZ and FCW designed the project. All authors performed the experiment. FZ drafted the manuscript. All authors read and approved the final manuscript.


The purpose of this study was to investigate the expression of carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) and correlate it with OPN expression and function in squamous carcinoma of tongue.
Paraffin were sections of 80 samples with squamous carcinoma of tongue and 40 samples with normal tissue of tongue for benign lesion having undergone surgery. Immunohistochemistry (IHC) was used to study the distribution of CEACAM5 and OPN, and double–labeling immunohistochemistry was used to observe the relationship between CEACAM5 and OPN expression.
CEACAM5 and OPN are found in normal tissue of tongue, but with different expression pattern. CEACAM5 expression mainly with membranous staining is restricted on the superficial epithelium. However, OPN expression with mainly cytoplasmic staining is restricted on the deep epithelium. No colocalization of CEACAM5 and OPN have been observed in normal tissue of tongue. In squamous carcinoma of tongue, CEACAM5 expression with cytoplasmic staining is different from normal tongue tissue with membranous staining, and the transformation of CEACAM5 distribution from membrane to cytoplasm is an important incident for the invasion and differentiation of tumor. CEACAM5 and OPN are colocalized in cytoplasm, and a significant correlation was observed between the positive colocalization and the negative colocalization in the depth of invasion and the differentiation of the tumor.
Authors’ original file for figure 1
Authors’ original file for figure 2
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