Background
H. pylori survives in the human gastric mucosa
H. pylori OMPLA implicated in colonization
The H. pylori acid defence mechanism hypothesis
Results
Survival of H. pylori OMPLA variants in acidic environment
H. pylori isolates | OMPLA length (amino acids) | Enzyme activity | Truncated OMPLA or missense mutation? | Sequence ID (GenBank ID) | |
---|---|---|---|---|---|
euBL (98019) | OMPLAON
| 355 | Yes | – | AFR51755.1 |
OMPLAOFF
| 263 | No | Truncated OMPLA | N/A | |
euAP (29A) | OMPLAON
| 355 | Yes | – | AFR51733.1 |
OMPLAOFF
| 265 | No | Truncated OMPLA | N/A | |
euBF (5A) | OMPLAON
| 355 | Yes | – | AFR51749.1 |
OMPLAOFF
| 263 | No | Truncated OMPLA | N/A | |
euBZ (9B) | OMPLAON
| 355 | Yes | – | AFR51769.1 |
OMPLAOFF
| 355 | No | Missense mutation (Pro157Ser) | N/A | |
euBB (53A) | OMPLAON
| 355 | Yes | – | AFR51745.1 |
OMPLAOFF
| 355 | No | Missense mutation (Ser235Arg) | N/A |
H. pylori OMPLA 3D structure model
Sequence similarities in acid tolerant species of the Helicobacteraceae family
Species | Representative strain | Classification | Predicted loop insert |
---|---|---|---|
H. acinonychis
| Sheeba | Gastric | Insert |
H. bizzozeronii
| CIII-1 | Gastric | Insert |
H. cetorum
| MIT 99-5656 | Gastric | Insert |
H. heilmannii
| ASB1.4 | Gastric | Insert |
H. pylori
| ATCC 43504 | Gastric | Insert |
H. suis
| HS5 (partially sequenced) | Gastric | Insert |
H. felis
| ATCC 49179 | Gastric | Insert |
H. mustelae
| 12198 | Gastric | NA |
H. himalayensis
| YS1 | Gastric | No insert |
H. bilis
| ATCC 43879 | Enterohepatic | Short insert |
H. canadensis
| MIT 98-5491 | Enterohepatic | No insert |
H. canis
| NCTC 12740 | Enterohepatic | No insert |
H. cinaedi
| CCUG 18818 = ATCC BAA-847 | Enterohepatic | No insert |
H. fennelliae
| MRY 12-0050 | Enterohepatic | Short insert |
H. hepaticus
| ATCC 51449 | Enterohepatic | No insert |
H. muridarum
| ST-1 | Enterohepatic | Short insert |
H. pullorum
| MIT 98-5489 | Enterohepatic | No insert |
H. rodentium
| ATCC 700285 | Enterohepatic | No insert |
H. trogontum
| ATCC 700114 | Enterohepatic | No insert |
H. typhlonicus
| MIT 97-6810 | Enterohepatic | No insert |
H. winghamensis
| ATCC BAA-430 | Enterohepatic | No insert |
In silico pldA operon prediction
Species | Representative strain | Host | Classification | Upstream | Downstream |
---|---|---|---|---|---|
H. acinonychis
| Sheeba | Cheetah | Gastric | AmCI and AmCII |
dnaN, gyrB, 2 hypothetical genes |
H. cetorum
| MIT 99-5656 | Dolphin | Gastric | AmCI* and AmCII |
dnaN, gyrB
|
H. pylori
| ATCC 43504 | Human | Gastric | AmCI and AmCII |
dnaN, gyrB, 2 hypothetical genes |
H. bizzozeronii
| CIII-1 | Human | Gastric | AmCI and AmCII | No |
H. himalayensis
| YS1 | Marmota himalayana | Gastric | No | No |
H. suis
| HS5 | Swine | Gastric | AmCI and AmCII | No |
H. felis
| ATCC 49179 | Cat | Gastric | AmCI and AmCII | No |
H. mustelae
| 12198 | Ferret | Gastric | No | No |
H. heilmannii
| ASB1.4 | Cat | Gastric | AmCI* and AmCII | No |
H. bilis
| ATCC 43879 | Human | Enterohepatic | No | No |
H. canadensis
| MIT 98-5491 | Human | Enterohepatic | No | No |
H. canis
| NCTC 12740 | Human | Enterohepatic | No |
pseH
|
H. cinaedi
| CCUG 18818 = ATCC BAA-847 | Human | Enterohepatic | No |
metE
|
H. fennelliae
| MRY 12-0050 | Human | Enterohepatic | Hypothetical | SAM-dependent MTase genes |
H. hepaticus
| ATCC 51449 | Mouse | Enterohepatic | No |
metE, and gene encoding NADPH |
H. muridarum
| ST-1 | Rat | Enterohepatic | No | ABC transporter gene |
H. pullorum
| MIT 98-5489 | Human | Enterohepatic | No | No |
H. rodentium
| ATCC 700285 | Mouse | Enterohepatic | No | No |
H. trogontum
| ATCC 700114 | Rat | Enterohepatic | No | No |
H. typhlonicus
| MIT 97-6810 | Mouse | Enterohepatic | No | No |
H. winghamensis
| ATCC BAA-430 | Human | Enterohepatic | No | No |