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Journal of Cancer Research and Clinical Oncology

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01.10.2010 | Original Paper

Overexpression of Galectin-1 is negatively correlated with pathologic differentiation grade in oral squamous cell carcinoma

verfasst von: Lai-ping Zhong, Kui-jie Wei, Xiao Yang, Hong-ya Pan, Dong-xia Ye, Li-zhen Wang, Zhi-yuan Zhang

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 10/2010

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Abstract

Purpose

To determine the Galectin-1 protein expression in oral squamous cell carcinoma (OSCC).

Methods

Comparative proteomic analysis of an in vitro cellular carcinogenesis model of OSCC we previously established was performed to identify differentially expressed proteins. Galectin-1 was further validated in vitro (human immortalized oral epithelia cell line and OSCC lines) and in vivo (tissue samples from OSCC patients) by Western blotting and immunohistochemistry, respectively.

Results

Increased Galectin-1 protein expression was identified in the cancerous cell line compared with the immortalized oral epithelial cell line in the in vitro cellular carcinogenesis model, and then validated in the OSCC lines and cancerous tissues. Galectin-1 protein expression was negatively correlated with the tumor pathologic differentiation grades, a higher Galectin-1 protein expression indicating a poorer pathologic differentiation grade.

Conclusions

Galectin-1 protein expression level increases in OSCC, it may serve as a candidate marker for pathologic differentiation grade of OSCC.

Barnes L, Eveson JW, Reichart P (2005) World health organization classification of tumours, pathology and genetics of head and neck tumours. IARC Press, Lyon, pp 168–175

Barondes SH, Castronovo V, Cooper DN et al (1994a) Galectins: a family of animal beta-galactoside-binding lectins. Cell 76:597–598CrossRefPubMed

Barondes SH, Cooper DN, Gitt MA et al (1994b) Galectins: structure and function of a large family of animal lectins. J Biol Chem 269:20807–20810PubMed

Camby I, Belot N, Lefranc F et al (2002) Galectin-1 modulates human glioblastoma cell migration into the brain through modifications to the actin cytoskeleton and levels of expression of small GTPases. J Neuropathol Exp Neurol 61:585–596PubMed

Chen J, He QY, Yuen AP et al (2004) Proteomics of buccal squamous cell carcinoma: the involvement of multiple pathways in tumorigenesis. Proteomics 4:2465–2475CrossRefPubMed

Chiang WF, Liu SY, Fang LY et al (2008) Overexpression of galectin-1 at the tumor invasion front is associated with poor prognosis in early-stage oral squamous cell carcinoma. Oral Oncol 44:325–334CrossRefPubMed

Choufani G, Nagy N, Saussez S et al (1999) The levels of expression of galectin-1, galectin-3, and the Thomsen-Friedenreich antigen and their binding sites decrease as clinical aggressiveness increases in head and neck cancers. Cancer 86:2353–2363CrossRefPubMed

Clausse N, van den Brule F, Waltregny D et al (1999) Galectin-1 expression in prostate tumor-associated capillary endothelial cells is increased by prostate carcinoma cells and modulates heterotypic cell-cell adhesion. Angiogenesis 3:317–325CrossRefPubMed

Fischer C, Sanchez-Ruderisch H, Welzel M et al (2005) Galectin-1 interacts with the {alpha}5{beta}1 fibronectin receptor to restrict carcinoma cell growth via induction of p21 and p27. J Biol Chem 280:37266–37277CrossRefPubMed

Gillenwater A, Xu XC, el-Naggar AK et al (1996) Expression of galectins in head and neck squamous cell carcinoma. Head Neck 18:422–432CrossRefPubMed

He QY, Chen J, Kung HF et al (2004) Identification of tumor-associated proteins in oral tongue squamous cell carcinoma by proteomics. Proteomics 4:271–278CrossRefPubMed

Koike H, Uzawa K, Nakashima D et al (2005) Identification of differentially expressed proteins in oral squamous cell carcinoma using a global proteomic approach. Int J Oncol 27:59–67PubMed

Kopitz J, von Reitzenstein C, Andre S et al (2001) Negative regulation of neuroblastoma cell growth by carbohydrate-dependent surface binding of galectin-1 and functional divergence from galectin-3. J Biol Chem 276:35917–35923CrossRefPubMed

Le QT, Kong C, Lavori PW et al (2007) Expression and prognostic significance of a panel of tissue hypoxia markers in head-and-neck squamous cell carcinomas. Int J Radiat Oncol Biol Phys 69:167–175PubMed

Lenaerts K, Bouwman FG, Lamers WH et al (2007) Comparative proteomic analysis of cell lines and scrapings of the human intestinal epithelium. BMC Genomics 8:91CrossRefPubMed

Lo WY, Tsai MH, Tsai Y et al (2007) Identification of over-expressed proteins in oral squamous cell carcinoma (OSCC) patients by clinical proteomic analysis. Clin Chim Acta 376:101–107CrossRefPubMed

Mlynarek AM, Balys RL, Su J et al (2007) A cell proteomic approach for the detection of secretable biomarkers of invasiveness in oral squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 133:910–918CrossRefPubMed

Neville BW, Day TA (2002) Oral cancer and precancerous lesions. CA Cancer J Clin 52:195–215CrossRefPubMed

Parkin DM, Bray F, Ferlay J et al (2005) Global cancer statistics, 2002. CA Cancer J Clin 55:74–108CrossRefPubMed

Paz A, Haklai R, Elad-Sfadia G et al (2001) Galectin-1 binds oncogenic H-Ras to mediate Ras membrane anchorage and cell transformation. Oncogene 20:7486–7493CrossRefPubMed

Rabinovich GA (2005) Galectin-1 as a potential cancer target. Br J Cancer 92:1188–1192CrossRefPubMed

Rorive S, Belot N, Decaestecker C et al (2001) Galectin-1 is highly expressed in human gliomas with relevance for modulation of invasion of tumor astrocytes into the brain parenchyma. Glia 33:241–255CrossRefPubMed

Saussez S, Cucu DR, Decaestecker C et al (2006) Galectin 7 (p53-induced gene 1): a new prognostic predictor of recurrence and survival in stage IV hypopharyngeal cancer. Ann Surg Oncol 13:999–1009CrossRefPubMed

Saussez S, Lorfevre F, Lequeux T et al (2008) The determination of the levels of circulating galectin-1 and -3 in HNSCC patients could be used to monitor tumor progression and/or responses to therapy. Oral Oncol 44:86–93CrossRefPubMed

Sdek P, Zhang ZY, Cao J et al (2006) Alteration of cell-cycle regulatory proteins in human oral epithelial cells immortalized by HPV16 E6 and E7. Int J Oral Maxillofac Surg 35:653–657CrossRefPubMed

Sobin LH, Wittekind CH (2002) International Union Against Cancer (UICC) TNM classification of malignant tumours, 6th edn. Wiley-Liss, New York, pp 22–26

Staab CA, Ceder R, Jägerbrink T et al (2007) Bioinformatics processing of protein and transcript profiles of normal and transformed cell lines indicates functional impairment of transcriptional regulators in buccal carcinoma. J Proteome Res 6:3705–3717CrossRefPubMed

Tinari N, Kuwabara I, Huflejt ME et al (2001) Glycoprotein 90 K/MAC-2BP interacts with galectin-1 and mediates galectin-1-induced cell aggregation. Int J Cancer 91:167–172CrossRefPubMed

Turhani D, Krapfenbauer K, Thurnher D et al (2006) Identification of differentially expressed, tumor-associated proteins in oral squamous cell carcinoma by proteomic analysis. Electrophoresis 27:1417–1423CrossRefPubMed

Wells V, Davies D, Mallucci L (1999) Cell cycle arrest and induction of apoptosis by beta galactoside binding protein (beta GBP) in human mammary cancer cells. A potential new approach to cancer control. Eur J Cancer 35:978–983CrossRefPubMed

Yamaoka K, Mishima K, Nagashima Y et al (2000) Expression of galectin-1 mRNA correlates with the malignant potential of human gliomas and expression of antisense galectin-1 inhibits the growth of 9 glioma cells. J Neurosci Res 59:722–730CrossRefPubMed

Zhong LP, Chen WT, Zhan CP et al (2007a) Increased CK19 expression correlated with pathological differentiation grade and prognosis in oral squamous cell carcinoma patients. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:377–384CrossRefPubMed

Zhong LP, Li J, Zhang CP et al (2007b) Expression of E-cadherin in cervical lymph nodes from primary oral squamous cell carcinoma patients. Arch Oral Biol 52:740–747CrossRefPubMed

Zhong LP, Zhang CP, Li J et al (2007c) Increase of Cyfra 21–1 concentration in saliva from oral squamous cell carcinoma patients. Arch Oral Biol 52:1079–1087CrossRefPubMed

Zhong LP, Pan HY, Zhou XJ et al (2008) Characteristics of a cancerous cell line, HIOEC-B(a)P-96, induced by benzo(a)pyrene from human immortalized oral epithelial cell line. Arch Oral Biol 53:443–452CrossRefPubMed

Zhong LP, Zhang L, Yang X et al (2009) Comparative proteomic analysis of differentially expressed proteins in an in vitro cellular carcinogenesis model of oral squamous cell carcinoma. Proteomics Clin Appl 3:322–337CrossRef

Titel: Overexpression of Galectin-1 is negatively correlated with pathologic differentiation grade in oral squamous cell carcinoma
verfasst von: Lai-ping Zhong
Kui-jie Wei
Xiao Yang
Hong-ya Pan
Dong-xia Ye
Li-zhen Wang
Zhi-yuan Zhang
Publikationsdatum: 01.10.2010
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 10/2010
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-010-0810-2

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Overexpression of Galectin-1 is negatively correlated with pathologic differentiation grade in oral squamous cell carcinoma

Abstract

Purpose

Methods

Results

Conclusions

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

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Abstract

Purpose

Methods

Results

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