Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice

Ovol2, a mouse homolog of Drosophila ovo, was identified as a zinc finger transcription factor predominantly expressed in testis [1]. Previous studies revealed that Ovol2 exhibits its functions in keratinocyte transient proliferation and differentiation [2], mouse embryonic stem cells differentiation [3] and primordial germ cell development [4]. However, the function of Ovol2 in postnatal male germ cell development remains enigmatic. Thus, we firstly examined the mRNA and protein levels of Ovol2 in multiple adult mouse tissues by RT-qPCR and western blot. We found that both the mRNA and protein of Ovol2 are highly expressed in testis and lung (Fig. 1a-c). We then examined the expression levels of Ovol2 in postnatal developing testes and found that both mRNA and protein of Ovol2 are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to adult testes (P56) and exhibits its highest level at adult testis (Fig. 1d-f). Further testicular immuno-staining revealed that OVOL2 is highly expressed in the germ cells (spermatogonia, spermatocytes and round spermatids) (Fig. 1g and Additional file 1: Figure S1A-D). Thus we hypothesized that Ovol2 could play an important role in postnatal germ cell development and spermatogenesis.

Due to Ovol2 conventional mutant mice displayed an embryonic mortality [4], we tried to specific knockout of Ovol2 in mouse germ cells to determine the physiological roles of Ovol2 in germ cell development. We then generated Ovol2 conditional knockout mouse model by crossing Ovol2-floxed mice with Vasa-cre (Cre was specifically activated at embryonic day 15.5) to inactive Ovol2 gene in testicular germ cells (Fig. 2a). The genotype of Ovol2 germ cell-specific knockout mice (Vasa-Cre; Ovol2^flox/flox, hereafter called Vasa-cKO) was confirmed by PCR-based genotyping analyses (Fig. 2b). In addition to genotyping analyses, both mRNA and proteins of Ovol2 were appeared to be significantly reduced in Vasa-cKO testes compared with that of WT controls by RT-qPCR, Western blot and Immunofluorescence analyses (Fig. 2c-e and Additional file 1: Figure S1E). Therefore, these data suggest that Ovol2 was specifically inactivated in testes efficiently.

To investigate the fertility of Vasa-cKO mice, we bred the Vasa-cKO males with fertility-proven WT females for at least 5 months. Unexpectedly, Vasa-cKO breeding pairs can produce comparable litter size to WT breeding pairs (data not shown), which indicated that Vasa-cKO male mice are completely fertile. Consistent with this fertile phenotype, testis gross morphology and weights are comparable between Vasa-cKO and WT control mice (Fig. 2f-g). Histological analyses further revealed that Vasa-cKO testes display normal spermatogenesis (Fig. 2h). To further confirm the Vasa-Cre recombined deletion efficiency in DNA level, we detected the delete allele of Ovol2 in the offspring derived from Vasa-cKO male breeding pairs by PCR-based DNA analyses. As we expected, all of pups are contained Ovol2 delete allele (Additional file 1: Figure S2). Together, these data indicate that Ovol2 is not essential for spermatogenesis and male germ cell development in mice despite its high expression in testis.

Ovol1, another Drosophila ovo mouse homologue, was confirmed to express in overlapping tissues with Ovol2, such as skin, kidney and testis. Ablation of Ovol1 in mice led to abnormal male germ cell development and male infertility, and Ovol1 is essential for spermatogenesis [5]. Thus, we analyzed the expression levels of Ovol1 in adult WT and Vasa-cKO testes by RT-qPCR. Interestingly, both Ovol1 mRNA and protein levels were significantly increased in Vasa-cKO testes compared with those of WT testes (Fig. 2i-k). Therefore, these data suggest that Ovol1 may have compensated for the loss of Ovol2 functions in germ cells, which leads to normal phenotype in Vasa-Cre;Ovol2^lox/lox mice. However, it is worthwhile pointing out that other transcription factors need to be elucidated in future, which may contribute to the compensation of OVOL2 loss-of -function in male germ cells. Overall, in this study, we report that Ovol2 is dispensable for testicular germ cell development and spermatogenesis in mice, and provide a molecular therapeutic clue for human male infertility caused by genetic mutation.

Both mRNA and protein of Ovol2 are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to adult testes (P56) and exhibits its highest level at adult testis. Ovol2 is dispensable for testicular germ cell development and spermatogenesis in mice. Ovol1 may have compensated for the loss of Ovol2 functions in germ cells, which leads to normal phenotype in Ovol2 conditional mutation mice.