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Erschienen in:
13.10.2022 | Original Article
Oxidative stress evaluation of post-vasectomised Kano Brown bucks following skin apposition with improvised suture materials and silk suture
Erschienen in: Comparative Clinical Pathology | Ausgabe 6/2022
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Surgical procedures cause oxidative stress, and the use of suture materials contributes to environmental stress factors. The aim of this study was to evaluate the oxidative stress effects of skin apposition with cobbler’s, embroidery, hair-braiding threads and silk suture using serum malondialdehyde (MDA) concentration and erythrocyte osmotic fragility (EOF) in Kano Brown (KB) bucks, post-vasectomy. Twenty, apparently, healthy Kano Brown bucks, aged between 6 and 18 months and live-weight of 10–15 kg, were used for the experiment. They were randomly assigned into four experimental groups of five bucks each. Vasectomy was performed on all the bucks after sedation with 0.05 mg/kg of xylazine intravenously, and linear infiltration of 5 mL of 2% lignocaine hydrochloride. Post-vasectomy, cutaneous skin apposition was performed with embroidery thread (ET) in group A bucks, cobbler’s thread (CT) in group B bucks, hair-braiding thread (HBT) in group C bucks and standard silk suture (SS) in group D bucks. The EOF and the serum MDA concentration were evaluated on days 2, 5, 14 and 28 post-vasectomy. At 0.5% NaCl, HBT-sutured bucks recorded the lowest (P < 0.05) percentage haemolysis (65 ± 2.6%), followed by the SS (68 ± 4.9%), CT (79 ± 3.5%) and ET (84 ± 2.5%). Elevated MDA recorded in bucks sutured with the experimental suture materials and the silk suture did not differ significantly (P > 0.05). It was deduced that vasectomy causes oxidative stress as evidenced by increased percentages of haemolysis in the following order: HBT < SS < CT < ET. The study has demonstrated, for the first time that the EOF may serve as additional diagnostic tool in the evaluation of oxidative stress changes in bucks, sutured with different suture materials post-vasectomy. In conclusion, HBT induced the least percentage of EOF, while ET, CT and HBT induced lower MDA concentration changes, they may be a good alternative to silk suture.
