Background
Methods
Patients
RNA extraction and quantitative real-time polymerase chain reaction
TCGA clinical and P3H4 data
Statistical method
Results
P3H4 was significantly upregulated in bladder cancer
P3H4 expression was associated with clinicopathological features in bladder cancer
Characteristics | Expression of P3H4 mRNA, number (%) | ||
---|---|---|---|
High (n = 195) | Low (n = 194) | p value | |
Age at diagnosis, years | 69.25 ± 10.18 | 66.39 ± 10.87 |
0.008
|
Gender | 0.053 | ||
Male | 136 (69.74) | 152 (78.35) | |
Female | 59 (30.26) | 42 (21.65) | |
BMI | 26.71 ± 4.78 | 27.42 ± 6.99 | 0.277 |
Race category |
0.001
| ||
White | 163 (87.63) | 145 (77.54) | |
Black or African American | 13 (6.99) | 9 (4.81) | |
Asian | 10 (5.38) | 33 (17.65) | |
Angiolymphatic invasion | 0.181 | ||
Yes | 77 (57.04) | 64 (48.85) | |
No | 58 (42.96) | 67 (51.15) | |
Extracapsular extension | 0.937 | ||
Yes | 35 (44.30) | 31 (43.66) | |
NO | 44 (55.70) | 40 (56.34) | |
Karnofsky performance score | 84.33 ± 12.46 | 80.67 ± 15.39 | 0.146 |
Histologic grade |
0.001
| ||
High | 191 (98.45) | 174 (90.63) | |
Low | 3 (1.55) | 18 (9.38) | |
Lymph node metastasis | 0.454 | ||
Yes | 59 (38.31) | 53 (42.74) | |
No | 95 (61.69) | 71 (57.26) | |
Tumor histologic subtype |
0.011
| ||
Non-papillary | 141 (73.82) | 119 (61.66) | |
Papillary | 50 (26.18) | 74 (38.34) | |
Metastasis | 0.154 | ||
M0 | 79 (91.86) | 110 (97.35) | |
M1 | 7 (8.14) | 3 (2.65) | |
AJCC stage |
0.006
| ||
I | 0 (0) | 1 (0.52) | |
II | 49 (25.13) | 79 (41.15) | |
III | 75 (38.46) | 55 (28.65) | |
IV | 71 (36.41) | 57 (29.69) |
P3H4 expression was significantly related to prognosis in bladder cancer
Clinicopathologic features | HR | 95% CI | p value |
---|---|---|---|
Age | 1.04 | 1.023–1.057 |
< 0.001
|
Gender | 1.08 | 0.771–1.513 | 0.655 |
BMI | 0.994 | 0.965–1.023 | 0.683 |
Race category | 1.158 | 0.862–1.555 | 0.33 |
Angiolymphatic invasion | 1.619 | 1.177–2.226 |
0.003
|
Extracapsular extension | 1.359 | 0.936–1.974 | 0.107 |
Karnofsky performance score | 0.998 | 0.976–1.021 | 0.893 |
Histologic grade | 2.853 | 0.705–11.542 | 0.141 |
Lymph node metastasis | 2.071 | 1.47–2.918 |
< 0.001
|
Tumor histologic subtype | 0.628 | 0.433–0.909 |
0.014
|
Metastasis | 4.507 | 2.131–9.532 |
< 0.001
|
AJCC stage | 1.708 | 1.402–2.08 |
< 0.001
|
P3H4 | 1.504 | 1.104–2.048 |
0.01
|
Clinicopathologic features | HR | 95% CI | p value |
---|---|---|---|
Age | 1.037 | 1.019–1.054 |
< 0.001
|
AJCC stage | 1.560 | 1.265–1.923 |
< 0.001
|
P3H4 | 1.413 | 1.016–1.966 |
0.04
|