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08.06.2018 | Review

Pancreatic cancer stem cells: features and detection methods

verfasst von: Toshiyuki Ishiwata, Yoko Matsuda, Hisashi Yoshimura, Norihiko Sasaki, Shunji Ishiwata, Naoshi Ishikawa, Kaiyo Takubo, Tomio Arai, Junko Aida

Erschienen in: Pathology & Oncology Research | Ausgabe 4/2018

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Abstract

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with a high incidence of distant metastasis and recurrence. Cancer stem cells (CSCs), which are pluripotent, self-renewable, and capable of forming tumors, contribute to PDAC initiation and metastasis and are responsible for resistance to chemotherapy and radiation. Three types of experimental methods are commonly used to identify CSCs: CSC-specific marker detection, a sphere-formation assay that reveals cell proliferation under non-adherent conditions, and detection of side-population (SP) cells that possess high intracellular-to-extracellular pump functions. Several CSC-specific markers have been reported in PDACs, including CD133, CD24, CD44, CXCR4, EpCAM, ABCG2, c-Met, ALDH-1, and nestin. There remains controversy regarding which markers are specific to PDAC CSCs and which are expressed alone or in combination in CSCs. Examining characteristics of isolated CSCs and discovering CSC-specific treatment options are important to improve the prognosis of PDAC cases. This review summarizes CSC-detection methods for PDAC, including CSC-marker detection, the sphere-formation assay, and detection of SP cells.

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Titel: Pancreatic cancer stem cells: features and detection methods
verfasst von: Toshiyuki Ishiwata
Yoko Matsuda
Hisashi Yoshimura
Norihiko Sasaki
Shunji Ishiwata
Naoshi Ishikawa
Kaiyo Takubo
Tomio Arai
Junko Aida
Publikationsdatum: 08.06.2018
Verlag: Springer Netherlands
Erschienen in: Pathology & Oncology Research / Ausgabe 4/2018
Print ISSN: 1219-4956
Elektronische ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-018-0420-x

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Eukaryotic Elongation Factor 2 (eEF2) is a Potential Biomarker of Prostate Cancer

Overexpression of FZD1 and CAIX are Associated with Invasion, Metastasis, and Poor-Prognosis of the Pancreatic Ductal Adenocarcinoma

The Impact of Routine Frozen Section Assessment During Penectomy on Surgical Margin Status and Long-Term Oncologic Outcomes

ISUP Group 4 – a Homogenous Group of Prostate Cancers?

Neu im Fachgebiet Onkologie

Prostatakarzinom: EU initiiert neues Screeningkonzept

Blasenkarzinom – Biomarker statt Zytologie?

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Adjuvante Brustkrebstherapie: Doppelt hält besser

Update Onkologie