Significance and innovations
Background
Research Methods
Subjects
Methods
Clinical data collection
Diagnostic criteria
MRI edema scoring
Fine needle aspiration biopsy
Autopsy specimens
Tissue processing
Immunohistochemistry analysis
Microscopic examination
Definition of pathologic changes in sacroiliitis
Statistical analysis
Results
General clinical data
Pathology of sacroiliitis
Sacroiliitis < 2 (N = 271) | Sacroiliitis ≥2 (N = 115) |
P
| |
---|---|---|---|
Chondrocyte degeneration, n/n* (%) | 52/234 (22.2) | 55/90 (61.1) |
0.000
|
Cartilage matrix degeneration, n/n* (%) | 103/234 (44.0) | 70/90 (77.8) |
0.000
|
Cartilage pannus invasion, n/n * (%) | 61/234 (26.1) | 52/90 (57.8) |
0.000
|
Cavity side pannus invasion, n/n * (%) | 7/234 (3.0) | 12/90 (13.3) |
0.001
|
Marrow side pannus invasion, n/n * (%) | 57/234 (24.4) | 51/90 (56.7) |
0.000
|
Endochondral ossification, n/n * (%) | 15/234 (6.4) | 25/90 (27.8) |
0.000
|
Subchondral pannus formation, n/n * (%) | 134/174 (77.0) | 70/75 (93.3) |
0.001
|
Subchondral bone disruption, n/n * (%) | 128/174 (73.6) | 69/75 (92.0) |
0.001
|
Osteoclast activation, n/n * (%) | 81/174(46.6) | 37/75 (49.3) | 0.065 |
Pathologic new bone formation, n/n * (%) | 47/174 (27.0) | 29/75 (38.7) | 0.155 |
Sequestrum, n/n * (%) | 7/174 (4.0) | 13/75 (17.3) |
0.000
|
Marrow inflammatory cell infiltration, n/n * (%) | 57/108 (52.8) | 19/54 (35.2) |
0.017
|
Synovitis, n/n * (%) | 8/27 (29.6) | 4/6 (66.7) | 0.173 |
Enthesitis, n/n * (%) | 5/31 (16.1) | 11/23 (47.8) |
0.019
|
Relationship between SIJ radiological structural damage progression during follow up and the manifested pathologic appearances at baseline
Pannus invasion and related inflammation
Pannus invasion (N = 26) | No pannus invasion (N = 24) |
P
| |
---|---|---|---|
Cartilage | |||
Chondrocyte degeneration, n (%) | 20 (76.9) | 5 (20.8) |
0.000
|
Cartilage matrix degeneration, n (%) | 23 (88.5) | 9 (37.5) |
0.000
|
Endochondral ossification, n (%) | 10 (38.5) | 5 (20.8) | 0.174 |
VEGF | |||
Scores | 2.0 ± 1.3 | 1.1 ± 1.2 |
0.021
|
Area of positive expression (μm2) | 657.4 ± 984.9 | 199.3 ± 418.8 |
0.022
|
Caspase-3 | |||
Scores | 2.3 ± 0.9 | 1.8 ± 1.7 | 0.419 |
Area of positive expression (μm2) | 620.0 ± 652.5 | 207.7 ± 285.2 | 0.185 |
MMP-3 | |||
Scores | 2.0 ± 1.1 | 0.7 ± 0.8 |
0.010
|
Area of positive expression (μm2) | 378.4 ± 406.0 | 50.3 ± 83.3 |
0.008
|
TNF-α | |||
Scores | 3.2 ± 1.2 | 1.6 ± 1.0 |
0.012
|
Area of positive expression (μm2) | 846.9 ± 739.2 | 181.1 ± 172.8 |
0.025
|
Bone-cartilage interface (n/40 hpf) | |||
CD34+ microvessels | 12.7 ± 3.6 | 7.4 ± 2.0 |
0.000
|
CD68+ macrophages | 10.1 ± 2.9 | 8.7 ± 3.5 | 0.277 |
CD68+ multinuclear osteoclasts | 2.3 ± 0.9 | 1.9 ± 0.9 | 0.228 |