nach oben

Metabolic Brain Disease

Erschienen in:

14.11.2019 | Original Article

Passiflora caerulea L. fruit extract and its metabolites ameliorate epileptic seizure, cognitive deficit and oxidative stress in pilocarpine-induced epileptic mice

verfasst von: G. Smilin Bell Aseervatham, E. Abbirami, T. Sivasudha, K. Ruckmani

Erschienen in: Metabolic Brain Disease | Ausgabe 1/2020

Einloggen, um Zugang zu erhalten

Abstract

The anticonvulsant potential of aqueous fruit extract of Passiflora caerulea (PCAE) was evaluated in swiss albino mice induced by pilocarpine. The antioxidant activities of PCAE were determined which showed strong antioxidant activity and the polyphenol compounds such as ginsenoside, naringenin, chrysoeriol 8-c-glucoside, luteolin-6-C-glucoside, apigenin-6,8-di-C-β-D-glucopyranoside were profiled through RP-HPLC and UPLC-ESI-MS/MS. Chronic effects of PCAE on pilocarpine (85 mg/kg; i.p)-induced convulsions were evaluated in Swiss adult male albino mice. PCAE at 100 and 200 mg/kg, (p.o.) and diazepam (5 mg/kg, i.p) were administered once daily for 15 days. In Y-maze test, percentage of correct entry by pilocarpine administered animals were significantly lower when compared to control, whereas PCAE at both doses improved the alteration score significantly. Administration of higher dose (200 mg/kg) of PCAE significantly delayed onset of convulsions and decreased duration of clonic convulsions. Association of ROS production during seizure period was further confirmed by histopathological studies revealing loss of normal neuronal cells in hippocampus region. The data obtained showed anticonvulsant activity and improved cognitive function; reduced the oxidative damage and significantly activated the cholinergic neurotransmission in a dose dependent manner similar to diazepam which is evident in the biochemical parameters and histopathological study, suggesting therapeutic potential for epilepsy and neurodegeneration.

Abaza MS, Bahman AM, Al-Attiyah RJ, Kollamparambil AM (2012) Synergistic induction of apoptosis and chemosensitization of human colorectal cancer cells by histone deacetylase inhibitor, scriptaid, and proteasome inhibitors: potential mechanisms of action. Tumor Biol 33(6):1951–1972CrossRef

Akhondzadeh S, Naghavi HR, Vazirian M, Shayeganpour A, Rashidi H, Khani M (2001) Passion flower in the treatment of generalized anxiety disorder: a pilot double-blind randomized controlled trial with oxazepam. J Clin Pharma Ther 26:363–367CrossRef

Alinejad B, Ghorbani A, Sadeghnia HR (2013) Effects of combination of curcumin, linalool, rutin, safranal and thymoquinone on glucose/serum deprivation-induced cell death. Avicenna J Phytomed 3:321–328PubMedPubMedCentral

Appel K, Rose T, Fiebich B, Kammler T, Hoffmann C, Weiss G (2011) Modulation of the γ-aminobutyric acid (GABA) system by Passiflora incarnata L. Phytother Res 25:838–843PubMedCrossRef

Aslanargun P, Cuvas O, Dikmen B, Aslan E, Yuksel EU (2012) Passiflora incarnate Linneaus as an anxiolytic before spinal anesthesia. J Anesth 26:39–44PubMedCrossRef

Bagheri M, Joghataei MT, Mohseni S, Roghani M (2011) Genistein ameliorates learning and memory deficits in amyloid β(1–40) rat model of Alzheimer’ s disease. Neurobiol Lear Mem 95:270–276CrossRef

Barbalho SM, Soares Souza MS, Silva JC, Mendes CJ, de Oliveira CA, Costa T, Farinazzi-Machado FM (2012) Yellow passion fruit rind (Passiflora edulis): an industrial waste or an adjuvant in the maintenance of glycemia and prevention of dyslipidemia? J Diab Res Clin Met 1:5CrossRef

Barros L, Oliveira S, Carvalho AM, Ferreira ICFR (2010) In vitro antioxidant properties and characterization in nutrients and phytochemicals of six medicinal plants from the Portuguese folk medicine. Ind Crop Prod 32:572–579CrossRef

Benbadis SR, Hauser WA (2000) An estimate of the prevalence of psychogenic non-epileptic seizures. Seizure 9:280–281PubMedCrossRef

Bugallo V, Cardone S, Pannunzio MJ, Facciuto G (2011) Breeding advances in Passiflora spp. (passion flower) native to Argentina. Floriculture Ornamental Biotech 5:23–34

Chen G, Chen S, Chen F, Xie Y, Han M, Luo C, Zhao Y, Gao Y (2016) Nutraceutical potential and antioxidant benefits of selected fruit seeds subjected to an in vitro digestion. J Funct Foods 20:317–331CrossRef

Chisté RC, Mercadante AZ, Gomes A, Fernandes E, Lima JLFC, Bragagnolo N (2011) In vitro scavenging capacity of annatto seed extracts against reactive oxygen and nitrogen species. Food Chem 127:419–426PubMedCrossRef

Chisté RC, Freitas M, Mercadante A, Fernandes E (2012) The potential of extracts of Caryocar villosum pulp to scavenge reactive oxygen and nitrogen species. Food Chem 135:1740–1749PubMedCrossRef

Cock HR (2002) The role of mitochondria and oxidative stress in neuronal damage after brief and prolonged seizures. Prog Brain Res 135:187–196PubMedCrossRef

Cuyckens F, Rozenberg R, de Hoffmann E, Claeys M (2001) Structure characterization of flavonoid O-diglycosides by positive and negative nano-electrospray ionization ion trap mass spectrometry. J Mass Spectrom 36:1203PubMedCrossRef

Dai J, Mumper RJ (2010) Plant Phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15:7313–7352PubMedPubMedCentralCrossRef

Das A, Shanker G, Nath C, Pal R, Singh S, Singh HK (2002) A comparative study in rodents of standardized extracts of Bacopa monniera and Ginkgo biloba anticholinesterase and cognitive enhancing activities. Pharmacol Biochem Behav 73:893–900PubMedCrossRef

Dong XX, Wang Y, Qin Z (2009) Molecular mechanisms of excitotoxicity and their relevance to pathogenesis of neurodegenerative diseases. Acta Pharma 30:379–387

Duthie GG, Duthie S, Kyle AM (2000) Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants. Nutr Res Rev 13:79–106PubMedCrossRef

Elsasa SM, Rossi DJ, White G, Seeleya CA, Gregoryd WI, Mohrb C, Pfankuch T, Soumyanatha A (2010) Passiflora incarnata L. (passionflower) extracts elicit GABA currents in hippocampal neurons in vitro, and show anxiogenic and anticonvulsant effects in vivo, varying with extraction method. Phytomed 17:940–949CrossRef

Engels C, Gräter D, Esquivel P, Jiménez VM, Gänzle MG, Schieber A (2012) Characterization of phenolic compounds in jocote (Spondias purpurea L.) peels by ultra high-performance liquid chromatography/electrospray ionization mass spectrometry. Food Res Int 46:557–562CrossRef

Esmaeilpour K, Sheibani V, Shabani M, Mirnajafi-Zadeh J (2017) Effect of low frequency electrical stimulation on seizure-induced short- and long-term impairments in learning and memory in rats. Physiol Behav 168:112–121PubMedCrossRef

Frantseva MV, Velazquez JL, Hwang PA, Carlen PL (2000) Free radical production correlates with cell death in an in vitro model of epilepsy. Eur J Neurosci 12:1431–1439PubMedCrossRef

Gallai V, Alberti A, Gallai B, Coppola F, Floridi A, Sarchielli P (2003) Glutamate and nitric oxide pathway in chronic daily headache: evidence from cerebrospinal fluid. Cephalalgia 23:166–174PubMedCrossRef

Green LC, Tannenbaum SR, Goldman P (1981) Nitrate synthesis in the germ free and conventional rat. Science 212:56–58PubMedCrossRef

Gupta RK, Kumar D, Chaudhary AK, Maithani M, Singh R (2012) Antidiabetic activity of Passiflora incarnata Linn. In streptozotocin-induced diabetes in mice. J Ethnopharmacol 139:801–806PubMedCrossRef

Hansen JT, Koeppen BM (2002) Atlas of neuroanatomy and neurophysiology. Selections from the netter collection of medical illustrations. Special ed. Icon Custom Communications, USA

Hesam F, Balali GR, Tehrani RT (2012) Evaluation of antioxidant activity of three common potato (Solanum tuberosum) cultivars in Iran. Avicenna J Phytomed 2:79–85PubMedPubMedCentral

Holzmann I, Filho CV, Mora TC, Caceres A, Martinez JV, Cruz SM, De Souza MM (2011) Evaluations of behavioral and pharmacological effects of hydroalcoholic extract of Valeriana prionophylla stand from Guatemala. Evid based complement Alternat med 1–9CrossRef

Huong NTT, Malsumato K, Kasai R, Yamasaki K, Watanabe H (1998) In vitro antioxidant activity of Vietnamese ginseng saponin and its components. Biolo Pharm Bull 21:978–981CrossRef

Ichimura T, Yamanaka A, Ichiba T, Toyokawa T, Kamada Y, Tamamura T (2006) Antihypertensive effect of an extract of Passiflora edulis rind in spontaneously hypertensive rats. Biosci Biotechnol Biochem 70:718–721PubMedCrossRef

Ingale AG, Hivrale AU (2010) Pharmacological studies of Passiflora sp. and their bioactive compounds. African J Plant Sci 4:417–426

Ingkaninan K, Temkitthawon P, Chuenchom K, Yuyaem T, Thongnoi W (2003) Screening for acetylcholinesterase inhibitory activity in plants used in Thai traditional rejuvenating and neurotonic remedies. J Ethanopharmacol 89:3602CrossRef

John A, Shahidi F (2010) Phenolic compounds and antioxidant activity of Brazil nut (Bertholletia excelsa). J Funct Foods 2:196–209CrossRef

Kakkar P, Dos B, Viswnathan PN (1984) A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 21:130–132PubMed

Karadenz F, Burdurlu HS, Koca N, Soyer Y (2005) Antioxidant activity of selected fruits and vegetables grown in Turkey. Turk J Agri For 29:297–303

Kasote DM, Katyare SS, Hegde MV, Bae H (2015) Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci 11:982–991PubMedPubMedCentralCrossRef

Kerksick C, Willoughby D (2005) The antioxidant role of glutathione and N-acetyl-cysteine supplements and exercise-induced oxidative stress. J Int Soc Sports Nutr 2(2):38–44PubMedPubMedCentralCrossRef

Kudin AP, Bimpong-Buta NY, Vielhaber S, Elger CE, Kunz WS (2004) Characterization of superoxide-producing sites in isolated brain mitochondria. Int J Biol Sci 279:4127–4135

Librizzi L, Noe F, Vezzani A, Curtis M, Ravizza T (2012) Seizure-induced brain-borne inflammation sustains seizure recurrence and blood–brain barrier damage. Ann Neurol 72:82–90PubMedCrossRef

Liu S, Cui M, Liu Z, Song F, Mo W (2004) Structural analysis of saponins from medicinal herbs using electrospray ionization tandem mass spectrometry. J Am Soc Mass Spectrom 15:133–141PubMedCrossRef

Liu DS, Zhou YH, Liang ES, Li W, Lin WW, Chen FF, Gao W (2013) Neuroprotective effects of the Chinese Yi-qi-BuShen recipe extract on injury of rat hippocampal neurons induced by hypoxia/reoxygenation. J Ethnopharmacol 145:168–174PubMedCrossRef

Lowry H, Rosebrough AJ, Farr AL, Randall RJ (1951) Protein measurements with the follin phenol reagent. J Biol Chem 193:265–275PubMedCrossRef

Magistretti J, Ma L, Shalinsky MH, Lin W, Klink R, Alonso A (2004) Spike patterning by Ca²⁺-dependent regulation of a muscarinic cation current in entorhinal cortex layer II neurons. J Neurophysiol 92:1644–1657PubMedCrossRef

Mahendran S, Thippeswamy BS, Veerapur VP, Badami S (2011) Anticonvulsant activity of embelin isolated from Embelia ribes. Phytomed 18:186–188CrossRef

Martinc B, Grabnar I, Vovk T (2012) The role of reactive species in epileptogenesis and influence of antiepileptic drug therapy on oxidative stress. Curr Neuropharmacol 10:328–343PubMedPubMedCentralCrossRef

Menard C, Bastianetto S, Quirion R (2013) Neuroprotecive effects of resveratrol and epigallocatechin gallate polyphenols are mediated by the activation of protein kinase C gamma. Front Cell Neurosci 7:281PubMedPubMedCentralCrossRef

Michler H, Laakmann G, Wagner H (2011) Development of an LC-MS method for simultaneous quantitation of amentoflavone and biapigenin, the minor and major biflavones from Hypericum perforatum L., in human plasma and its application to real blood. Phytochem Anal 22:42–50PubMedCrossRef

Mori K, Togashi H, Ueno KI, Matsumoto M, Yoshioka M (2001) Aminoguanidine prevented the impairment of learning behavior and hippocampal long-term potentiation following transient cerebral ischemia. Behav Brain Res 120:159–168PubMedCrossRef

Nassiri-Asl M, Shariati-Rad S, Zamansoltani F (2007) Anticonvulsant effects of aerial parts of Passiflora incarnata extract in mice: involvement of benzodiazepine and opioid receptors. BMC Complement Altern Med 7:26PubMedPubMedCentralCrossRef

Ola SS, Catia G, Marzia I, Francesco VF, Afolabi AA, Nadia M (2009) HPLC/DAD/MS characterization and analysis of flavonoids and cynnamoil derivatives in four Nigerian green- leafy vegetables. Food Chem 115:1568–1574CrossRef

Oliveira AA, Almeida JPC, Freitas RM, Nascimento VS, Aguiar LMV, Junior HVN, Fonseca F, Viana GSB, Sousa FCF, Fonteles MMF (2007) Effects of levetiracetam in lipid peroxidation level, nitrite–nitrate formation and antioxidant enzymatic activity in mice brain after pilocarpine-induced seizures. Cell Mol Neurobio 27:395–406CrossRef

Pachauria SD, Totab S, Khandelwala K, Vermae PRP, Nathc C, Hanifb K, Shuklab R, Saxenad JK, Dwivedia AK (2012) Protective effect of fruits of Morinda citrifolia L. on scopolamine induced memory impairment in mice: a behavioral, biochemical and cerebral blood flow study. J Ethnopharmacol 139:34–41CrossRef

Pastore A, Federici G, Bertini E, Piemonte F (2003) Analysis of glutathione:implication in redox and detoxification. Clin Chim Acta 333:19–39PubMedCrossRef

Patil AS (2010) Exploring Passiflora incarnata L.: a medicinal plants secondary metabolites as antibacterial agent. J Med Plants Res 4:1496–1501

Quintans LJ, Almeida JRGS, Lima JT, Nunes XP, Siqueria JS, Oliveira LEGO, Almeida RN, Athayde-Filho PF, Filho JMB (2008) Plants with anticonvulsant properties—a review. Braz J Pharmacog 18:798–819CrossRef

Racine R, Okujava V, Chipashvili S (1972) Modification of seizure activity by electrical stimulation 3 mechanisms. Electroencephalogr Clin Neurophysiol 32:295–299PubMedCrossRef

Radenovic L, Selakovic V (2005) Differential effects of NMDA and AMPA/kainate receptor antagonists on nitric oxide production in rat brain following intra hippocampal injection. Brain Res Bull 67:133–141PubMedCrossRef

Ramaiya SD, Bujang JS, Zakaria MH (2014) Assessment of total phenolic, antioxidant, and antibacterial activities of passiflora species. Sci World J 2014:1–10

Rameshkumar A, Sivasudha T, Jeyadevi R, Sangeetha B, Smilin Bell Aseervatham G, Maheshwari M (2013) Profiling of phenolic compounds using UPLC-Q-TOF-MS/MS and nephroprotective activity of Indian green leafy vegetable Merremia emarginata (Burm. F.). Food Res Int 50:94–101CrossRef

Raza SS, Khan MM, Ahmad A, Ashafaq M, Islam F, Wagner AP, Safhi MM, Islam F (2013) Neuroprotective effect of naringenin is mediated through suppression of NF-κB signaling pathway in experimental stroke. Neurosci 230:157–171CrossRef

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Bio Med 26:1231–1237CrossRef

Reginatto FH, De-Paris F, Petry RD, Quevedo J, Ortega GG, Gosmann G, Schenkel EP (2006) Evaluation of anxiolytic activity of spray dried powders of two south Brazilian Passiflora species. Phytother Res 20:348–351PubMedCrossRef

Sadasivam S, Manickam A (1996) Biochemical methods. New age international (P) limited, publishers. New Delhi 8:193–194

Salahdeen HM, Yemitan OK (2006) Neuropharmacological effects of aqueous leaf extract of Bryophyllum pinnatum in mice. Afr J Biol Med Res 9:101–107

Sanchez-Rabaneda F, Jauregui O, Casals I, Andres-Lacueva C, Izquierdo-Pulido M, Lamuela-Raventos RM (2003) Liquid chromatographic/electrospray ionization tandem mass spectrometric study of the phenolic composition of cocoa (Theobroma cacao). J Mass Spectrom 38:35–42PubMedCrossRef

Santos IMS, Tome AR, Saldanha GB, Ferreira PMP, Militao GCGM, Freitas RM (2009) Oxidative stress in the hippocampus during experimental seizures can be ameliorated with the antioxidant ascorbic acid. Oxidative Med Cell Longev 2(4):214–221CrossRef

Saravanan S, Parimelazhagan T (2014) In vitro antioxidant, antimicrobial and anti-diabetic properties of polyphenols of Passiflora ligularis Juss. Fruit pulp. Food Science & Human Wellness 3:56–64CrossRef

Shen Y, Jin L, Xiao P, Lu Y, Bao JS (2009) Total phenolics, flavonoids, antioxidant capacity in rice grain and their relations to grain color, size and weight. J Cereal Sci 49:106–111CrossRef

Shin E, Jeong JH, Chung YH, Kim WK, Ko KH, Bach JH, Hong JS, Yoneda Y, Kim HC (2011) Role of oxidative stress in epileptic seizures. Neurochem Int 59:122–137PubMedPubMedCentralCrossRef

Silva B, Ferreres F, Malva JO, Dias ACP (2005) Phytochemical and antioxidant characterization of Hypericum perforatum alcoholic extracts. Food Chem 90:157–167CrossRef

Singh RP, Chidambaramurthy KN, Jayaprakash GK (2002) Studies on antioxidant polyphenol content of aqueous extracts from pomegranate peel and seed extracts using in vitro models. J Agri Food Chem 50:86–89

Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47:389–394PubMedCrossRef

Smilin Bell A, Sasikumar JM, Kumar D (2012) Studies on In vitro free radical scavenging activity of Bixa orellana L. bark extract. Int J Pharm Pharmceut Sci 4:719–726

Soman S, Korah PK, Jayanarayanan J, Mathew J, Paulose CS (2012) Oxidative stress induced NMDA receptor alteration leads to spatial memory deficits in temporal lobe epilepsy: ameliorative effects of Withania somnifera and withanolide a. Neurochem Res 37:1915–1927PubMedCrossRef

Sudha K, Rao AV, Rao A (2001) Oxidative stress and antioxidants in epilepsy. Clin Chim Act 303:19–24CrossRef

Tejada S, Roca C, Sureda A, Rial RV, Gamund A, Esteban S (2006) Antioxidant response analysis in the brain after pilocarpine treatments. Brain Res Bull 69:587–592PubMedCrossRef

Tran TD, Jackson HD, Horn HD, Goodlett CR (2005) Vitamin E does not protect against neonatal ethanol-induced cerebellar damage or deficits in eye blink classical conditioning in rats. Alcohol Clin Exp Res 29:117–129PubMedCrossRef

Uttara B, Singh AV, Zamboni P, Mahajan RT (2009) Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 7:65–74PubMedPubMedCentralCrossRef

Waseem M, Paevez S (2015) Neuropritective activities of curcumin and quercetin with potential relevance to mitochondrial dysfunction induced by oxaliplatin. Protoplasma 253(2):417–430PubMedCrossRef

Xu X, Ma C, Han Y, Li Y, Liu C, Duan Z, Wang H, Liu D, Liu R (2015) Protective effect of naringenin on glutamate-induced neurotoxicity in cultured hippocampal cells. Arch Biol Sci Belgrade 67:639–646CrossRef

Yu EA, Kim GS, Jeong SW, Park S, Lee SJ, Kim JH, Lee WS, Bark KM, Jin JS, Shin SC (2014) Flavonoid profile and biological activity of Korean citrus varieties (II): Pyunkyul (Citrus tangerina Hort. Ex Tanaka) and overall contribution of its flavonoids to antioxidant effect. J Funct Foods 6:637–642.ssCrossRef

Yusuf S, Adelaiye BA, Agunu A (2009) Effect of Ziziphus mauritiania (L.) seed extracts on spatial recognition memory of rats as measured by the Y maze test. J Nat Prod 2:31–39

Titel: Passiflora caerulea L. fruit extract and its metabolites ameliorate epileptic seizure, cognitive deficit and oxidative stress in pilocarpine-induced epileptic mice
verfasst von: G. Smilin Bell Aseervatham
E. Abbirami
T. Sivasudha
K. Ruckmani
Publikationsdatum: 14.11.2019
Verlag: Springer US
Erschienen in: Metabolic Brain Disease / Ausgabe 1/2020
Print ISSN: 0885-7490
Elektronische ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-019-00501-5

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

23.04.2024 | Demenz | Nachrichten

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Passiflora caerulea L. fruit extract and its metabolites ameliorate epileptic seizure, cognitive deficit and oxidative stress in pilocarpine-induced epileptic mice

Abstract

Leitlinien kompakt für die Neurologie

Neu im Fachgebiet Neurologie

Demenzkranke durch Antipsychotika vielfach gefährdet

Parkinson-Therapie im Wandel – aktuelle Leitlinie im Fokus

Auf diese Krankheiten bei Geflüchteten sollten Sie vorbereitet sein

Hustenstiller lindert Agitation bei Alzheimer

Update Neurologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2020

The role of non-coding RNAs in neuroprotection and angiogenesis following ischemic stroke

Preclinical and clinical research on the toxic and neurological effects of cassava (Manihot esculenta Crantz) consumption

Profiling of differentially expressed circular RNAs in peripheral blood mononuclear cells from Alzheimer’s disease patients

Protective effect of Oxymatrine against acute spinal cord injury in rats via modulating oxidative stress, inflammation and apoptosis

Serum agrin and talin are increased in major depression while agrin and creatine phosphokinase are associated with chronic fatigue and fibromyalgia symptoms in depression

Potential application of helminth therapy for resolution of neuroinflammation in neuropsychiatric disorders

Leitlinien kompakt für die Neurologie

Neu im Fachgebiet Neurologie

Demenzkranke durch Antipsychotika vielfach gefährdet

Parkinson-Therapie im Wandel – aktuelle Leitlinie im Fokus

Auf diese Krankheiten bei Geflüchteten sollten Sie vorbereitet sein

Hustenstiller lindert Agitation bei Alzheimer

Update Neurologie