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16.02.2019 | Original Article

Passive transfer of allergic encephalomyelitis in rats: a tool for drug mechanism studies and detecting late-acting immunosuppressants

Zeitschrift:
Inflammopharmacology
Autoren:
B. B. Newbould, C. M. Pearson, M. W. Whitehouse
Wichtige Hinweise
B. B. Newbould Deceased 25 November 2016, formerly ICI Pharmaceuticals, Macclesfield, Cheshire, UK.
C. M. Pearson Deceased 31 May 1981, formerly Director of Rheumatology, UCLA.

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Abstract
1.
A strategy is described for evaluating drugs against different phases in the development of an auto allergic disease, experimental allergic encephalomyelitis. It is based on a cell transfer technique whereby the disease is passively transferred with lymphoid cells from actively immunized donor rats to normal syngeneic rats = passive recipients. Drugs may be applied in vivo to either the cell donors or the cell recipients or to cells in vitro whilst in transit; their efficiency being determined by the severity of the passive disease (weight loss, paralysis) in the recipients.
 
2.
Examples are given illustrating the application of these techniques to:
(a)
evaluating the lymphocyte-deactivating activity of various nitrogen mustards in vitro;
 
(b)
recognizing drugs, e.g. gold derivatives, clofazimine, etc. that are not conventional immunosuppressant (or cytostatic) agents which, when given to the recipient animals, may prevent the expression of the adopted disease;
 
(c)
comparing some known immunosuppressants for potency, duration of action, etc.;
 
(d)
demonstrating the versatility of cycloleucine, ICI-47,776, etc.
 
 
3.
Some merits of the strategy are discussed vis a vis using the local graft-versus-host reaction in rats to search for new drugs.
 

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