nach oben

Comparative Clinical Pathology

Erschienen in:

05.01.2019 | Original Article

Pathological and hematological studies on the effect of curcumin on manganese chloride-induced neurotoxicity in rats

verfasst von: Marwa F. Ali, Mokhtar Taha

Erschienen in: Comparative Clinical Pathology | Ausgabe 1/2019

Einloggen, um Zugang zu erhalten

Abstract

The current work was undertaken to evaluate the toxicologic effect of manganese chloride (MnCl₂) and potential therapeutic effect of curcumin on male albino rats. Ninety-six rats were divided into three groups. MnCl₂ toxicity group (I) included 32 rats were administered (MnCl₂) at a dose of 5 mg/ml daily in drinking water orally for 8 weeks. Curcumin-treated group (II) included 32 rats were given (MnCl₂) in the same dose and route as the first group. Each rat of this group was treated by oral administration of curcumin in a daily dose 100 mg/kg b.w. The control group (III) included 32 rats. Eight rats were randomly selected from groups I, II and III and sacrificed at (2, 4, 6, and 8 weeks) of experiment. Tissue samples from cerebellum and midbrain of all groups were collected for histopathological examination, and neuromorphometrical analysis as well as blood samples were taken for complete blood picture. The histopathological examination in group I showed Purkinje cell loss in the cerebellum with degeneration of motor neurons in midbrain. In group II, curcumin caused significant increase in number of healthy Purkinje cells in cerebellum with prevalence of healthy motor neurons and minimal vascular alterations in midbrain. The hematological results revealed that animals in group I showed a significant increase in WBCs and platelets compared to other groups, while RBCs count significantly decreased. The number of poikilocyts increased in group I compared to group II and group III. In conclusion, curcumin therapy has a powerful neuroprotective effect in Mncl₂ toxicity.

Aggarwal BB, Harikumar KB (2009) Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol 41(1):40–59CrossRefPubMed

Agthong S, Kaewsema A, Charoensub T (2015) Curcumin ameliorates functional and structural abnormalities in cisplatin-induced neuropathy. Exp Neurol 24(2):139–145

Ajibade A, Fakunle P, Fatoba O, Olayemi O (2011) Some effects of manganese dichloride administration on the body weight, Purkinje cell population, brain, and cerebellar weights of adult Wistar rats. J Neurosci Behav Health 3(7):87–90

Al Moundhri MS, Al-Salam S, Al Mahrouqee A, Beegam S, Ali BH (2013) The effect of curcumin on oxaliplatin and cisplatin neurotoxicity in rats: some behavioral, biochemical, and histopathological studies. J Med Toxicol 9(1):25–33CrossRefPubMed

Alizadeh A, Dyck S, Karimi-Abdolrezaee S (2015) Myelin damage and repair in pathologic CNS: challenges and prospects. Front Mol Neurosci 8:1–27CrossRef

Antony S, Kuttan R, Kuttan G (1999) Immunomodulatory activity of curcumin. Immunol Investig 28(5–6):291–303CrossRef

Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB (2007) Bioavailability of curcumin: problems and promises. Molecular pharmaceutics. 4(6):807–818

Asai A, Miyazawa T (2000) Occurrence of orally administered curcuminoid as glucuronide and glucuronide/sulfate conjugates in rat plasma. Life Sci 67(23):2785–2793CrossRefPubMed

Aschner M, Guilarte TR, Schneider JS, Zheng W (2007) Manganese: recent advances in understanding its transport and neurotoxicity. Toxicol Appl Pharmacol 221(2):131–147CrossRefPubMedPubMedCentral

Ates B, Orun I, Talas ZS, Durmaz G, Yilmaz I (2008) Effects of sodium selenite on some biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) exposed to Pb2+ and Cu2+. Fish Physiol Biochem 34(1):53–59CrossRefPubMed

Attia AM, Ibrahim FA, EL-Latif NAA, Aziz SW (2014) Antioxidant effects of curcumin against cadmium chloride-induced oxidative stress in the blood of rats. J Pharmacogn Phytother 6(3):33–40CrossRef

Au C, Benedetto A, Aschner M (2008) Manganese transport in eukaryotes: the role of DMT1. Neurotoxicology 29(4):569–576CrossRefPubMedPubMedCentral

Babij R, Lee M, Cortes E, Vonsattel JP, Faust PL (2013) Purkinje cell axonal anatomy: quantifying morphometric changes in essential tremor versus control brains. Brain 136(10):3051–3061CrossRefPubMedPubMedCentral

Bahar E, Lee G-H, Bhattarai KR, Lee H-Y, Kim H-K, Handigund M, Choi M-K, Han S-Y, Chae H-J, Yoon H (2017) Protective role of quercetin against manganese-induced injury in the liver, kidney, and lung; and hematological parameters in acute and subchronic rat models. Drug Des Devel Ther 11:2605–2619CrossRefPubMedPubMedCentral

Bancroft JD, Stevens A (1982) Theory and practice of histological techniques. Churchill Livingstone, NY, pp 188–190

Barres BA (2008) The mystery and magic of glia: a perspective on their roles in health and disease. Neuron 60(3):430–440CrossRefPubMed

Boudou F, Aldi D, Slimani M, Berroukche A (2014) The impact of chronic exposure to manganese on testiculaire tissue and sperm parameters in rat Wistar. Int J Nat Sci Res 3:12–19

Brenneman KA, Wong BA, Buccellato MA, Costa ER, Gross EA, Dorman DC (2000) Direct olfactory transport of inhaled manganese (54MnCl2) to the rat brain: toxicokinetic investigations in a unilateral nasal occlusion model. Toxicol Appl Pharmacol 169(3):238–248CrossRefPubMed

Brown BA, Hunter RC, O'Hare A, Erim G (1993) Hematology: principles and procedures. In: Lea & Febiger Philadelphia, p 1–453

Buffolo M, Pei S, Pereira-Pires KM, Paulini J, Jorge L, Boudina S (2016) Neuronal deletion of manganese superoxide dismutase altered TCA cycle metabolism and caused severe peripheral nerve demyelination in mice. Free Radic Biol Med 100:158–159CrossRef

Chandel M, Jain GC (2016) Manganese-induced hematological alteration in Wistar rats. J Environ Occup Sci 5(4):77CrossRef

Chandra SV, Shukla GS (1978) Manganese encephalopathy in growing rats. Environ Res 15(1):28–37CrossRefPubMed

Charash B, Placek E, Sos TA, Kligfield P (1982) Dose-related effects of manganese on the canine electrocardiogram. J Electrocardiol 15(2):149–152CrossRefPubMed

Cho JY, Rhee MH, Hong S, Kwon M, Kim SH, Kang SY (2007) Neuroprotective effect of curcumin is mainly mediated by blockade of microglial cell activation. Die Pharmazie-an international. J Pharm Sci 62(12):937–942

Ciftci O, Ozdemir I, Tanyildizi S, Yildiz S, Oguzturk H (2011) Antioxidative effects of curcumin, β-myrcene and 1, 8-cineole against 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in rat’s liver. Toxicol Ind Health 27(5):447–453CrossRefPubMed

Crossgrove J, Zheng W (2004) Manganese toxicity upon overexposure. NMR Biomed 17(8):544–553CrossRefPubMedPubMedCentral

Crossgrove JS, Allen DD, Bukaveckas BL, Rhineheimer SS, Yokel RA (2003) Manganese distribution across the blood–brain barrier: I. Evidence for carrier-mediated influx of manganese citrate as well as manganese and manganese transferrin. Neurotoxicology 24(1):3–13CrossRefPubMed

Das, Prasad A, Sukla LB, Pradhan N (2012) Microbial recovery of manganese using Staphylococcus epidermidis. Int J Nonferrous Metall 1(02):9–15CrossRef

Devi P, Baruah D, Baruah B, Borkotoki A (2008) Impact of endosulfan on some haematological parameters of Channa punctatus (Bloch). Pollut Res 27(3):485–488

El-Boshy ME, Risha EF, Abdelhamid FM, Mubarak MS, Hadda TB (2015) Protective effects of selenium against cadmium induced hematological disturbances, immunosuppressive, oxidative stress and hepatorenal damage in rats. J Trace Elem Med Biol 29:104–110CrossRefPubMed

Erdogan S, Celik S, Erdogan Z (2004) Seasonal and locational effects on serum, milk, liver and kidney chromium, manganese, copper, zinc, and iron concentrations of dairy cows. Biol Trace Elem Res 98(1):51–61CrossRefPubMed

Erikson KM, Asschner M (2003) Manganese neurotoxicty and glutamate-GABA interaction. Neurochem Int 43(4):475–480CrossRefPubMed

Fernsebner K, Zorn J, Kanawati B, Walker A, Michalke B (2014) Manganese leads to an increase in markers of oxidative stress as well as to a shift in the ratio of Fe (II)/(III) in rat brain tissue. Metallomics 6(4):921–931CrossRefPubMed

Franco MCP, Dantas APV, Akamine EH, Kawamoto EM, Fortes ZB, Scavone C, Tostes RC, Carvalho MHC, Nigro D (2002) Enhanced oxidative stress as a potential mechanism underlying the programming of hypertension in utero. J Cardiovasc Pharmacol 40(4):501–509CrossRef

Gangarapu V, Gujjala S, Korivi R, Pala I (2013) Combined effect of curcumin and vitamin E against CCl4 induced liver injury in rats. American Journal of Life Sciences 1(3):117–124CrossRef

Gavin C, Gunter K, Gunter T (1999) Manganese and calcium transport in mitochondria: implications for manganese toxicity. Neurotoxicology 20(2):445–453PubMed

Hamai D, Campbell A, Bondy SC (2001) Modulation of oxidative events by multivalent manganese complexes in brain tissue. Free Radic Biol Med 31(6):763–768CrossRefPubMed

He Z, Liao Y, Zheng M, Zeng F-D, Guo L-J (2008) Piracetam improves cognitive deficits caused by chronic cerebral hypoperfusion in rats. Cell Mol Neurobiol 28(4):613–627CrossRefPubMed

He L, Peng X, Zhu J, Liu G, Chen X, Tang C, Liu H, Liu F, Peng Y (2015a) Protective effects of curcumin on acute gentamicin-induced nephrotoxicity in rats. Can J Physiol Pharmacol 93(4):275–282CrossRefPubMed

He Y, Yue Y, Zheng X, Zhang K, Chen S, Du Z (2015b) Curcumin, inflammation, and chronic diseases: how are they linked? Molecules 20(5):9183–9213CrossRefPubMedPubMedCentral

IBM Corp Released (2013) IBM SPSS statistics for Windows, version 22.0. Armonk, NY: IBM Corp

Imbrasienë D, Ðvedienë L, Paunksnis A, Abdrachmanovas O (2002) Vascular system changes among the workers in surroundings contaminated with manganese. Ekologija 4:3–6

Indravathi G, Devi C (2014) Manganese induced hematological alterations in albino rats: reversal effect of alpha-tocopherol. Int J Innov Res Sci Eng Technol 3(14988–14996)

Jayakumar A, Rao KR, Kalaiselvi P, Norenberg M (2004) Combined effects of ammonia and manganese on astrocytes in culture. Neurochem Res 29(11):2051–2056CrossRefPubMed

Jiao Y, Wilkinson J, Di X, Wang W, Hatcher H, Kock ND, D'Agostino R, Knovich MA, Torti FM, Torti SV (2009) Curcumin, a cancer chemopreventive and chemotherapeutic agent, is a biologically active iron chelator. Blood 113(2):462–469CrossRefPubMedPubMedCentral

Kamarudin TA, Othman F, Ramli ESM, Isa NM, Das S (2012) Protective effect of curcumin on experimentally induced arthritic rats: detailed histopathological study of the joints and white blood cell count. EXCLI J 11(226)

King MD, McCracken DJ, Wade FM, Meiler SE, Alleyne CH Jr, Dhandapani KM (2011) Attenuation of hematoma size and neurological injury with curcumin following intracerebral haemorrhage in mice. J Neurosurg 115(1):116–123CrossRefPubMedPubMedCentral

Klüver H, Barrera E (1953) A method for the combined staining of cells and fibers in the nervous system. J Neuropathol Exp Neurol 12(4):400–403CrossRefPubMed

Krishnakumar I, Maliakel A, Gopakumar G, Kumar D, Maliakel B, Kuttan R (2015) Improved blood–brain-barrier permeability and tissue distribution following the oral administration of a food-grade formulation of curcumin with fenugreek fibre. J Funct Foods 14:215–225CrossRef

Kuo C-P, Lu C-H, Wen L-L, Cherng C-H, Wong C-S, Borel CO, Ju D-T, Chen C-M, Wu C-T (2011) Neuroprotective effect of curcumin in an experimental rat model of subarachnoid hemorrhage. Anesthesiology 115(6):1229–1238PubMed

Lacramioara SI, Hogas M, Ciobica A, Dobrin R, Petrariu F (2014) The effects of chronic manganese administration on blood pressure in rats. Archives of Biological Sciences 66(1):157–160CrossRef

Lazrishvili I, Shukakidze A, Chkhartishvili N, Bikashvili T (2009) Morphological changes and manganese content in the brains of rat pups subjected to subchronic poisoning with manganese chloride. Neurosci Behav Physiol 39(1):7–12CrossRefPubMed

Lee B, Pine M, Johnson L, Rettori V, Hiney JK, Les Dees W (2006) Manganese acts centrally to activate reproductive hormone secretion and pubertal development in male rats. Reprod Toxicol 22(4):580–585CrossRefPubMed

Lin M-S, Lee Y-H, Chiu W-T, Hung K-S (2011) Curcumin provides neuroprotection after spinal cord injury. J Surg Res 166(2):280–289CrossRefPubMed

Liu X, Sullivan KA, Madl JE, Legare M, Tjalkens RB (2006) Manganese-induced neurotoxicity: the role of astroglial-derived nitric oxide in striatal interneuron degeneration. Toxicol Sci 91(2):521–531CrossRefPubMed

Mahmoud EA (2013) Effect of curcumin on hematological, biochemical and antioxidants parameters in Schistosoma mansoni infected mice. Int J Sci 2(2013–03):1–14

Manogaran E, Ramanathan M, RamaRao T (2015) Neuroprotective effect of curcumin against cholesterol induced neuroinflammation in-vitro and in-vivo models. J Pharm Sci Res 7(4):189–196

Michalke B, Halbach S, Nischwitz V (2007) Speciation and toxicological relevance of manganese in humans. J Environ Monit 9(7):650–656CrossRefPubMed

Misselwitz B, MÜhler A, Weinmann H-j (1995) A toxicologic risk for using manganese complexes: a literature survey of existing data through several medical specialties. Investig Radiol 30(10):611–620CrossRef

Nelson K, Golnick J, Korn T, Angle C (1993) Manganse encephalopathy: utility of early magnetic resonance imaging. Occup Environ Med 50(6):510–513CrossRef

Noorafshan A, Omidi A, Karbalay-Doust S (2011) Curcumin protects the dorsal root ganglion and sciatic nerve after crush in rat. Pathol Res Pract 207(9):577–582CrossRefPubMed

Packham MA (1994) Role of platelets in thrombosis and hemostasis. Can J Physiol Pharmacol 72(3):278–284CrossRefPubMed

Peeyush KT, Gireesh G, Jobin M, Paulose C (2009) Neuroprotective role of curcumin in the cerebellum of streptozotocin-induced diabetic rats. Life Sci 85(19):704–710CrossRefPubMed

Rajakrishnan V, Viswanathan P, Rajasekharan K, Menon VP (1999) Neuroprotective role of curcumin from curcuma longa on ethanol-induced brain damage. Phytother Res 13(7):571–574CrossRefPubMed

Ringman JM, Frautschy SA, Cole GM, Masterman DL, Cummings JL (2005) A potential role of the curry spice curcumin in Alzheimer’s disease. Curr Alzheimer Res 2(2):131–136CrossRefPubMedPubMedCentral

Romero AJ, Carbia C, Ceballo M, Diaz N (1999) Red cell distribution width (RDW): its use in the characterization of microcytic and hypochromic anemias. Medicina 59(1):17

Saijo K, Winner B, Carson CT, Collier JG, Boyer L, Rosenfeld MG, Gage FH, Glass CK (2009) A Nurr1/CoREST pathway in microglia and astrocytes protects dopaminergic neurons from inflammation-induced death. Cell 137(1):47–59CrossRefPubMedPubMedCentral

Sakonlaya D, Apisarnthanark A, Yamada N, Tomtitchong P (2014) Modified toluidine blue: an alternative stain for helicobacter pylori detection in routine diagnostic use and post-eradication confirmation for gastric cancer prevention. Asian Pac J Cancer Prev 15:6983–6987CrossRefPubMed

Samaka R, Girgis N, Shams T (2012) Acute toxicity and dependence of tramadol in albino rats: relationship of nestin and notch 1 as stem cell markers. J Am Sci 8(6):313–327

Samini F, Samarghandian S, Borji A, Mohammadi G (2013) Curcumin pretreatment attenuates brain lesion size and improves neurological function following traumatic brain injury in the rat. Pharmacol Biochem Behav 110:238–244CrossRefPubMed

Schmitt C, Strazielle N, Richaud P, Bouron A, Ghersi-Egea JF (2011) Active transport at the blood-CSF barrier contributes to manganese influx into the brain. J Neurochem 117(4):747–756PubMed

Sharma J, Langer S (2014) Effect of manganese on haematological parameters of fish, Garra gotyla gotyla. J Entomol Zool Stud 2(3):77–81

Sharma RA, Euden SA, Platton SL, Cooke DN, Shafayat A, Hewitt HR, Marczylo TH, Morgan B, Hemingway D, Plummer SM (2004) Phase I clinical trial of oral curcumin. Clin Cancer Res 10(20):6847–6854CrossRefPubMed

Sharma V, Sharma C, Sharma S (2011) Influence of curcuma longa and curcumin on blood profile in mice subjected to aflatoxin B1. Int J Pharm Sci Res 2(7):1740

Sidhu P, Nehru B (2004) Lead intoxication: histological and oxidative damage in rat cerebrum and cerebellum. J Trace Elem Exp Med 17(1):45–53CrossRef

Smith EA, Newland P, Bestwick KG, Ahmed N (2013) Increased whole blood manganese concentrations observed in children with iron deficiency anaemia. J Trace Elem Med Biol 27(1):65–69CrossRefPubMed

Stokes AH, Lewis DY, Lash LH, Jerome WG, Grant KW, Aschner M, Vrana KE (2000) Dopamine toxicity in neuroblastoma cells: role of glutathione depletion by L-BSO and apoptosis. Brain Res 858(1):1–8CrossRefPubMed

Stokinger HE (1981) The metals. In Patty’s industrial hygiene and toxicology, (1st Eds), 46–55

Sun Y, Dai M, Wang Y, Wang W, Sun Q, Yang G-Y, Bian L (2011) Neuroprotection and sensorimotor functional improvement by curcumin after intracerebral haemorrhage in mice. J Neurotrauma 28(12):2513–2521CrossRefPubMedPubMedCentral

Tehranipour M, Javaheri R (2009) Neuroprotective effect of curcuma longa alcoholic extract on peripheral nerves degeneration after sciatic nerve compression in rats. J Biol Sci 9(8):889–893CrossRef

Trujillo J, Chirino YI, Molina-Jijón E, Andérica-Romero AC, Tapia E, Pedraza-Chaverrí J (2013) Renoprotective effect of the antioxidant curcumin: recent findings. Redox Biol 1(1):448–456CrossRefPubMedPubMedCentral

Underwood E (2012) Trace elements in human and animal nutrition. In (Eds). Elsevier:

Unnikrishnan M, Rao M (1995) Curcumin inhibits nitrogen dioxide induced oxidation of hemoglobin. Mol Cell Biochem 146(1):35–37CrossRefPubMed

Vachharajani V, Wang S, Mishra N, El Gazzar M, Yoza B, McCall C (2010) Curcumin modulates leukocyte and platelet adhesion in murine sepsis. Microcirculation 17(6):407–416PubMedPubMedCentral

Vander Elst L, Colet J-m, Muller RN (1997) Spectroscopic and metabolic effects of MnCl2 and MnDPDP on the isolated and perfused rat heart. Investig Radiol 32(10):581–588CrossRef

Venkatesan N, Punithavathi D, Arumugam V (2000) Curcumin prevents adriamycin nephrotoxicity in rats. Br J Pharmacol 129(2):231–234CrossRefPubMedPubMedCentral

Villalobos V, Bonilla E, Castellano A, Novo E, Caspersen R, Giraldoth D, Medina-Leendertz S (2009) Ultrastructural changes of the olfactory bulb in manganese-treated mice. Biocell 33(3):187–197PubMed

Wang Y-F, Zu J-N, Li J, Chen C, Xi C-Y, Yan J-L (2014) Curcumin promotes the spinal cord repair via inhibition of glial scar formation and inflammation. Neurosci Lett 560:51–56CrossRefPubMed

Weiser G, O'Grady M (1983) Erythrocyte volume distribution analysis and hematologic changes in dogs with iron deficiency anemia. Vet Pathol 20(2):230–241CrossRefPubMed

Wongeakin N, Sridulyakul P, Jariyapongskul A, Suksamrarn A, Patumraj S (2009) Effects of curcumin and tetrahydrocurcumin on diabetes induced endothelial dysfunction. Afr J Biochem Res 3(5):259–265

Yamada M, Ohno S, Okayasu I, Okeda R, Hatakeyama S, Watanabe H, Ushio K, Tsukagoshi H (1986) Chronic manganese poisoning: a neuropathological study with determination of manganese distribution in the brain. Acta Neuropathol 70(3–4):273–278CrossRefPubMed

Yasuba M, Okimoto K, Iida M, Itakura C (1988) Cerebellar cortical degeneration in beagle dogs. Vet Pathol 25(4):315–317CrossRefPubMed

Yu H, Ma L, Jiang J, Sun S (2016) Protective effect of curcumin on neural myelin sheaths by attenuating interactions between the endoplasmic reticulum and mitochondria after compressed spinal cord. J Spine 5(4):1–6

Zhao F, Cai T, Liu M, Zheng G, Luo W, Chen J (2008) Manganese induces dopaminergic neurodegeneration via microglial activation in a rat model of manganism. Toxicol Sci 107(1):156–164CrossRefPubMed

Titel: Pathological and hematological studies on the effect of curcumin on manganese chloride-induced neurotoxicity in rats
verfasst von: Marwa F. Ali
Mokhtar Taha
Publikationsdatum: 05.01.2019
Verlag: Springer London
Erschienen in: Comparative Clinical Pathology / Ausgabe 1/2019
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-018-02888-6

Neu im Fachgebiet Pathologie

01.04.2024 | Forensische Traumatologie | CME

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Pathological and hematological studies on the effect of curcumin on manganese chloride-induced neurotoxicity in rats

Abstract

Neu im Fachgebiet Pathologie

Theoretische Grundlagen von Explosionen und Explosionsverletzungen

Auswirkungen vorgeschalteter Laborprozesse auf die Digitalisierung histologischer Schnittpräparate

Knochenmarkhistologie bei Zytopenien

Herausforderungen der Automation bei der quantitativen Auswertung von Leberbiopsien

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2019

Adenosine deaminase behavior in experimental infection by Brucella ovis and its participation in the modulation of the inflammatory response

Correction to: Subclinical nodular goiter associated with Hurthle cell, papillary, and adenomatoid hyperplasic nodules in the dromedary camel in the Sultanate of Oman

Methylation pattern and mutational status of BRCA1 in canine mammary tumors in a Brazilian population

Anatomy and histomorphology of thyroid, parathyroid and ultimobranchial glands in Guinea fowl (Numida meleagris)

Evaluation of the toxicopathological lesions of Calotropis procera using a chick embryonic model

Applications of fluorescence in situ hybridization in detection of disease biomarkers and personalized medicine

Neu im Fachgebiet Pathologie

Theoretische Grundlagen von Explosionen und Explosionsverletzungen

Auswirkungen vorgeschalteter Laborprozesse auf die Digitalisierung histologischer Schnittpräparate

Knochenmarkhistologie bei Zytopenien

Herausforderungen der Automation bei der quantitativen Auswertung von Leberbiopsien