nach oben

European Journal of Nuclear Medicine and Molecular Imaging

Erschienen in:

24.07.2019 | Short Communication

PET imaging of metabolic changes after neural stem cells and GABA progenitor cells transplantation in a rat model of temporal lobe epilepsy

verfasst von: Ruili Du, Xiandi Zhu, Shuang Wu, Xiaohui Zhang, Yang He, Kai Zhang, Xiao He, Xiaoqun Wang, Yujie Sun, Qiangbin Wang, Hong Zhang, Mei Tian

Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging | Ausgabe 11/2019

Einloggen, um Zugang zu erhalten

Abstract

Purpose

Stem cell transplantation is promising for temporal lobe epilepsy (TLE) treatment. This study aimed to use PET imaging for the investigation of dynamic metabolic changes after transplantation of human neural stem cells (NSCs) and human GABA progenitor cells (GPCs) in a rat model of TLE.

Methods

¹⁸F-FDG PET imaging, video-electroencephalography (EEG), whole-cell patch-clamp recordings and immunostaining were performed after transplantation of NSCs and GPCs.

Results

PET imaging demonstrated that glucose metabolism was gradually improved in the NSCs group, but decreased in GPCs and the control. Video-EEG manifested that seizures were suppressed after NSCs or GPCs transplantation; whole-cell patch-clamp confirmed increased inhibitory response of GPC-derived cells; immunostaining studies verified that the transplanted NSCs and GPCs could survive, migrate and differentiate into mature neuronal subtypes.

Conclusion

¹⁸F-FDG PET imaging could be a distinguishing approach for evaluation of dynamic glycolytic metabolic changes after transplantation of NSCs and GPCs in TLE. Whole-cell patch-clamp provides evidence for functional maturation and integration of transplanted stem cells within host circuits.

Nur mit Berechtigung zugänglich

Chen Z, Brodie MJ, Liew D, Kwan P. Treatment outcomes in patients with newly diagnosed epilepsy treated with established and new antiepileptic drugs: a 30-year longitudinal cohort study. JAMA Neurol. 2018;75(3):279–86.CrossRefPubMed

Waldau B, Hattiangady B, Kuruba R, Shetty AK. Medial ganglionic eminence-derived neural stem cell grafts ease spontaneous seizures and restore GDNF expression in a rat model of chronic temporal lobe epilepsy. Stem Cells. 2010;28:1153–64.PubMedPubMedCentral

Upadhya D, Hattiangady B, Castro OW, Shuai B, Kodali M, Attaluri S, et al. Human induced pluripotent stem cell-derived MGE cell grafting after status epilepticus attenuates chronic epilepsy and comorbidities via synaptic integration. Proc Natl Acad Sci USA. 2019;116(1):287–96.CrossRefPubMed

Miltiadous P, Kouroupi G, Stamatakis A, Koutsoudaki PN, Matsas R, Stylianopoulou F. Subventricular zone-derived neural stem cell grafts protect against hippocampal degeneration and restore cognitive function in the mouse following intrahippocampal kainic acid administration. Stem Cells Transl Med. 2013;2(3):185–98.CrossRefPubMedPubMedCentral

Zhu Y, Feng J, Wu S, Hou H, Ji J, Zhang K, et al. Glucose metabolic profile by visual assessment combined with statistical parametric mapping analysis in pediatric patients with epilepsy. J Nucl Med. 2017;58(8):1293–9.CrossRefPubMed

Ding Y, Zhu Y, Jiang B, Zhou Y, Jin B, Hou H, et al. (18)F-FDG PET and high-resolution MRI co-registration for pre-surgical evaluation of patients with conventional MRI-negative refractory extra-temporal lobe epilepsy. Eur J Nucl Med Mol Imaging. 2018;45(9):1567–72.CrossRefPubMed

Xu JC, Fan J, Wang X, Eacker SM, Kam TI, Chen L, et al. Cultured networks of excitatory projection neurons and inhibitory interneurons for studying human cortical neurotoxicity. Sci Transl Med. 2016;8(333):333ra48.CrossRefPubMedPubMedCentral

Liu Y, Liu H, Sauvey C, Yao L, Zarnowska ED, Zhang S-C. Directed differentiation of forebrain GABA interneurons from human pluripotent stem cells. Nat Protoc. 2013;8(9):1670–9.CrossRefPubMedPubMedCentral

Racine RJ. Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr Clin Neurophysiol. 1972;32(3):281–94.CrossRef

10.

Kornblum HI, Araujo DM, Annala AJ, Tatsukawa KJ, Phelps ME, Cherry SR. In vivo imaging of neuronal activation and plasticity in the rat brain by high resolution positron emission tomography (microPET). Nat Biotechnol. 2000;18(6):655–60.CrossRef

11.

Baraban SC, Southwell DG, Estrada RC, Jones DL, Sebe JY, Alfaro-Cervello C, et al. Reduction of seizures by transplantation of cortical GABAergic interneuron precursors into Kv1.1 mutant mice. Proc Natl Acad Sci USA. 2009;106(36):15472–7.CrossRefPubMed

12.

Van Paesschen W, Dupont P, Sunaert S, Goffin K, Van Laere K. The use of SPECT and PET in routine clinical practice in epilepsy. Curr Opin Neurol. 2007;20(2):194–202.CrossRefPubMed

13.

Cunningham M, Cho JH, Leung A, Savvidis G, Ahn S, Moon M, et al. hPSC-derived maturing GABAergic interneurons ameliorate seizures and abnormal behavior in epileptic mice. Cell Stem Cell. 2014;15(5):559–73.CrossRefPubMedPubMedCentral

14.

Waldvogel D, van Gelderen P, Muellbacher W, Ziemann U, Immisch I, Hallett M. The relative metabolic demand of inhibition and excitation. Nature. 2000;406(6799):995–8.CrossRefPubMed

15.

Lu P, Ceto S, Wang Y, Graham L, Wu D, Kumamaru H, et al. Prolonged human neural stem cell maturation supports recovery in injured rodent CNS. J Clin Investig. 2017;127(9):3287–99.CrossRefPubMed

Titel: PET imaging of metabolic changes after neural stem cells and GABA progenitor cells transplantation in a rat model of temporal lobe epilepsy
verfasst von: Ruili Du
Xiandi Zhu
Shuang Wu
Xiaohui Zhang
Yang He
Kai Zhang
Xiao He
Xiaoqun Wang
Yujie Sun
Qiangbin Wang
Hong Zhang
Mei Tian
Publikationsdatum: 24.07.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging / Ausgabe 11/2019
Print ISSN: 1619-7070
Elektronische ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-019-04408-2

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 11/2019

Al18F-NOTA-octreotide: first comparison with 68Ga-DOTATATE in a neuroendocrine tumour patient

A multiparametric analysis based on DCE-MRI to improve the accuracy of parotid tumor discrimination

Low-calorie sweeteners augment tissue-specific insulin sensitivity in a large animal model of obesity

Personalised radioembolization improves outcomes in refractory intra-hepatic cholangiocarcinoma: a multicenter study

The use of 68Ga-PET/CT PSMA in the staging of primary and suspected recurrent renal cell carcinoma

Current experts’ views on precision nuclear medicine imaging of phaeochromocytoma and paraganglioma