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01.11.2010 | Research | Ausgabe 1/2010 Open Access

Gut Pathogens 1/2010

PFGE, Lior serotype, and antimicrobial resistance patterns among Campylobacter jejuni isolated from travelers and US military personnel with acute diarrhea in Thailand, 1998-2003

Zeitschrift:
Gut Pathogens > Ausgabe 1/2010
Autoren:
Oralak Serichantalergs, Piyarat Pootong, Anders Dalsgaard, Ladaporn Bodhidatta, Patricia Guerry, David R Tribble, Sinn Anuras, Carl J Mason
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1757-4749-2-15) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

OS designed and carried out the study project (including the data analysis and preparation of the draft manuscript), PP performed the PFGE of the C. jejuni isolates, AD provided ideas and comments on the draft manuscript, LB analyzed the epidemiological data for the C. jejuni isolates, PG provided expertise on the molecular biology of C. jejuni, DT directed the patient recruitment in the US military exercise, SA supported the study project etiology of acute diarrhea at Bumrungrad Hospital, and CJM conceived the idea for the study (and performed statistical analysis and worked on the final manuscript). All authors read and approved the final manuscript.

Abstract

Background

Campylobacter jejuni is a major cause of gastroenteritis worldwide. In Thailand, several strains of C. jejuni have been isolated and identified as major diarrheal pathogens among adult travelers. To study the epidemiology of C. jejuni in adult travelers and U.S. military personnel with acute diarrhea in Thailand from 1998-2003, strains of C. jejuni were isolated and phenotypically identified, serotyped, tested for antimicrobial susceptibility, and characterized using pulsed-field gel electrophoresis (PFGE).

Results

A total of 312 C. jejuni isolates were obtained from travelers (n = 46) and U.S. military personnel (n = 266) in Thailand who were experiencing acute diarrhea. Nalidixic acid and ciprofloxacin resistance was observed in 94.9% and 93.0% of the isolates, respectively. From 2001-2003, resistance to tetracycline (81.9%), trimethoprim-sulfamethoxazole (57.9%), ampicillin (28.9%), kanamycin (5.9%), sulfisoxazole (3.9%), neomycin (2.0%), and streptomycin (0.7%) was observed. Combined PFGE analysis showed considerable genetic diversity among the C. jejuni isolates; however, four PFGE clusters included isolates from the major Lior serotypes (HL: 36, HL: 11, HL: 5, and HL: 28). The PFGE analysis linked individual C. jejuni clones that were obtained at U.S. military exercises with specific antimicrobial resistance patterns.

Conclusions

In summary, most human C. jejuni isolates from Thailand were multi-resistant to quinolones and tetracycline. PFGE detected spatial and temporal C. jejuni clonality responsible for the common sources of Campylobacter gastroenteritis.
Zusatzmaterial
Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 5
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Literatur
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