nach oben

18.12.2017 | Original Article | Ausgabe 4/2018

Photostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source

Zeitschrift:: Lasers in Medical Science > Ausgabe 4/2018

Autoren:: Anıl Sera Çakmak, Soner Çakmak, H. Seda Vatansever, Menemşe Gümüşderelioğlu

Abstract

Low-level laser therapy (LLLT) has been used for more than 30 years to heal wounds. In recent years, LLLT or photostimulation has been indicated as an effective tool for regenerative and dental medicine by using monochromatic light. The aim of this study is to indicate the usability of plasma arc light source for bone regeneration. This is why we used polychromatic light source providing effective wavelengths in the range of 590–1500 nm for cellular response and investigated photostimulation effects on osteogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D silk scaffolds. Cellular responses were examined by using cell culture methods in terms of proliferation, differentiation, and morphological analyses. The results showed that photostimulation with a polychromatic light source (applied for 5 min from the 3rd day after seeding up to the 28th day in 2-day intervals with 92-mW/cm² power from 10-cm distance to the cells) enhanced osteogenic differentiation of hMSCs according to higher alkaline phosphatase (ALP) activity, collagen and calcium content, osteogenic gene expressions, and matrix mineralization. In conclusion, we suggest that the plasma arc light source that was used here has a great potential for bone regeneration.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Jetzt einloggen Kostenlos registrieren

★ PREMIUM-INHALT

e.Med Interdisziplinär

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Fachzeitschriften auf SpringerMedizin.de. Zusätzlich können Sie eine Zeitschrift Ihrer Wahl in gedruckter Form beziehen – ohne Aufpreis.

Jetzt gratis testen * Jetzt kaufen

Das kostenlose Testabonnement läuft nach 14 Tagen automatisch und formlos aus. Dieses Abonnement kann nur einmal getestet werden.

e.Dent - Das Online-Abo für Zahnärzte

Online-Abonnement

Mit e.Dent erhalten Sie Zugang zu allen zahnmedizinischen Fortbildungen und unseren zahnmedizinischen und ausgesuchten medizinischen Zeitschriften.

Jetzt testen ¹

Lacroix D, Prendergast PJ, Li G, Marsh D (2002) Biomechanical model to simulate tissue differentiation and bone regeneration: application to fracture healing. Med Biol Eng Comput 40:14–21 CrossRefPubMed

Chao EY, Inoue N (2003) Biophysical stimulation of bone fracture repair, regeneration and remodelling. Eur Cell Mater 6:72–84 CrossRefPubMed

Çakmak AS, Çakmak S, Kim K, Yiğit S, White JD, Raja WK, Kaplan DL, Gümüşderelioğlu M (2016) Synergistic effect of exogeneous and endogeneous electrostimulation on osteogenic differentiation of human mesenchymal stem cells seeded on silk scaffolds. J Orthop Res 34:581–590 CrossRefPubMed

Yu W, Naim JO, McGowan M, Ippolito K, Lanzafame RJ (1997) Photomodulation of oxidative metabolism and electron chain enzymes in rat liver mitochondria. Photochem Photobiol 66:866–871 CrossRefPubMed

Karu TI (2003) Low-power laser therapy. CRC Press, USA CrossRef

Conlan MJ, Rapley JW, Cobb CM (1996) Biostimulation of wound healing by low-energy laser irradiation. A review. J Clin Periodontol 23:492–496 CrossRefPubMed

Assia E, Rosner M, Belkin M, Solomon A, Schwartz M (1989) Temporal parameters of low energy laser irradiation for optimal delay of post-traumatic degeneration of rat optic nerve. Brain Res 476:205–212 CrossRefPubMed

Bibikova A, Oron U (1993) Promotion of muscle regeneration in the toad (Bufo viridis) gastrocnemius muscle by low-energy laser irradiation. Anat Rec 235:374–380 CrossRefPubMed

Weiss N, Oron U (1992) Enhancement of muscle regeneration in the rat gastrocnemius muscle by low energy laser irradiation. Anat Embryol 186:497–503 CrossRefPubMed

10.

Kawasaki K, Shimizu N (2000) Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats. Lasers Surg Med 26:282–291 CrossRefPubMed

11.

Kim HK, Kim JH, Abbas AA, Kim DO, Park SJ, Chung JY, Song EK, Yoon TR (2009) Red light of 647 nm enhances osteogenic differentiation in mesenchymal stem cells. Lasers Med Sci 24:214–222 CrossRefPubMed

12.

Soleimani M, Abbasnia E, Fathi M, Sahraei H, Fathi Y, Kaka G (2012) The effects of low-level laser irradiation on differentiation and proliferation of human bone marrow mesenchymal stem cells into neurons and osteoblasts—an in vitro study. Lasers Med Sci 27:423–430 CrossRefPubMed

13.

Abramovitch-Gottlib L, Gross T, Naveh D, Geresh S, Rosenwaks S, Bar I, Vago R (2005) Low level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix. Lasers Med Sci 20:138–146 CrossRefPubMed

14.

Karu T (1999) Primary and secondary mechanisms of action of visible-to-near IR radiation on cells. J Photochem Photobiol B Biol 49:1–17 CrossRef

15.

Karu TI (2008) Mitochondrial signaling in mammalian cells activated by red and near-IR radiation. Photochem Photobiol 84:1091–1099 CrossRefPubMed

16.

Almeida-Lopes L, Rigau J, Zangaro RA, Guidugli-Neto J, Jaeger MM (2001) Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med 29:179–184 CrossRefPubMed

17.

AlGhamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237–249 CrossRefPubMed

18.

Fujihara NA, Hiraki KR, Marques MM (2006) Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med 38:332–336 CrossRefPubMed

19.

Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterials 26:3503–3509 CrossRefPubMed

20.

Gan L, Tse C, Pilliar RM, Kandel RA (2007) Low-power laser stimulation of tissue engineered cartilage tissue formed on a porous calcium polyphosphate scaffold. Lasers Surg Med 39:286–293 CrossRefPubMed

21.

Parenti SI, Panseri S, Gracco A, Sandri M, Tampieri A, Bonetti GA (2013) Effect of low-level laser irradiation on osteoblast-like cells cultured on porous hydroxyapatite scaffolds. Ann Ist Super Sanita 49:255–260

22.

Ülker N, Çakmak AS, Kiremitçi A, Gümüşderelioğlu M (2016) Polychromatic light-induced osteogenic activity in 2d and 3d cultures. Lasers Med Sci 31:1665–1674 CrossRefPubMed

23.

Bhumiratana S, Grayson WL, Castaneda A, Rockwood DN, Gil ES, Kaplan DL, Vunjak-Novakovic G (2011) Nucleation and growth of mineralized bone matrix on silk-hydroxyapatite composite scaffolds. Biomaterials 32:2812–2820 CrossRefPubMedPubMedCentral

24.

Nazarov R, Jin HJ, Kaplan DL (2004) Porous 3-D scaffolds from regenerated silk fibroin. Biomacromolecules 5:718–726 CrossRefPubMed

25.

Meinel L, Karageorgiou V, Hofmann S, Fajardo R, Snyder B, Li C, Zichner L, Langer R, Vunjak-Novakovic G, Kaplan DL (2004) Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. J Biomed Mater Res A 71:25–34 CrossRefPubMed

26.

Tuby H, Maltz L, Oron U (2007) Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture. Lasers Surg Med 39:373–378 CrossRefPubMed

27.

Quickenden T, Daniels L (1993) Attempted biostimulation of division in Saccharomyces cerevisiae using red coherent light. Photochem Photobiol 57:272–278 CrossRefPubMed

28.

Schneede P, Jelkmann W, Schramm U, Fricke H, Steinmetz M, Hofstetter A (1988) Effects of the helium-neon laser on rat kidney epithelial cells in culture. Lasers Med Sci 3:249–257 CrossRef

29.

Renno ACM, McDonnell PA, Crovace MC, Zanotto ED, Laakso L (2010) Effect of 830 nm laser phototherapy on osteoblasts grown in vitro on biosilicate® scaffolds. Photomed Laser Surg 28:131–133 CrossRefPubMed

30.

Bouvet-Gerbettaz S, Merigo E, Rocca JP, Carle GF, Rochet N (2009) Effects of low-level laser therapy on proliferation and differentiation of murine bone marrow cells into osteoblasts and osteoclasts. Lasers Surg Med 41:291–297 CrossRefPubMed

31.

Romijn JC, Verkoelen CF, Schroeder FH (1988) Application of the MTT assay to human prostate cancer cell lines in vitro: establishment of test conditions and assessment of hormone-stimulated growth and drug-induced cytostatic and cytotoxic effects. Prostate 12:99–110 CrossRefPubMed

32.

Teng S-H, Lee F-J, Yoon B-H, Shin D-S, Kim H-E, Oh J-S (2009) Chitosan/nanohydroxyapatite composite membranes via dynamic filtration for guided bone regeneration. J Biomed Mater Res A 88:569–580 CrossRefPubMed

33.

Wu JY, Chen CH, Yeh LY (2013) Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate. Int J Oral Sci 5:85–91 CrossRefPubMedPubMedCentral

34.

Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161–166 CrossRefPubMed

35.

Ueda Y, Shimizu N (2003) Effects of pulse frequency of low-level laser therapy (LLLT) on bone nodule formation in rat calvarial cells. J Clin Laser Med Surg 21:271–277 CrossRefPubMed

36.

Mizuno M, Kuboki Y (2001) Osteoblast-related gene expression of bone marrow cells during the osteoblastic differentiation induced by type I collagen. J Biochem 129:133–138 CrossRefPubMed

37.

Hawkins D, Houreld N, Abrahamse H (2005) Low level laser therapy (LLLT) as an effective therapeutic modality for delayed wound healing. Ann N Y Acad Sci 1056:486–493 CrossRefPubMed

38.

Pires D, Xavier M, Araujo T, Silva JA, Aimbire F, Albertini R (2011) Low-level laser therapy (LLLT; 780 nm) acts differently on mRNA expression of anti- and pro-inflammatory mediators in an experimental model of collagenase-induced tendinitis in rat. Lasers Med Sci 26:85–94 CrossRefPubMed

39.

Bloise N, Ceccarelli G, Minzioni P, Vercellino M, Benedetti L, De Angelis MG (2013) Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors. J Biomed Opt 18:1–13 CrossRef

40.

Coombe AR, Ho CT, Darendeliler MA, Hunter N, Philips JR, Chapple CC (2001) The effects of low level laser irradiation on osteoblastic cells. Clin Orthod Res 4:3–14 CrossRefPubMed

41.

Zayzafoon M (2006) Calcium/calmodulin signaling controls osteoblast growth and differentiation. J Cell Biochem 97:56–70 CrossRefPubMed

42.

Sundelacruz S, Levin M, Kaplan DL (2008) Membrane potential controls adipogenic and osteogenic differentiation of mesenchymal stem cells. PLoS One 3:1–15 CrossRef

43.

Ryoo HM, Lee MH, Kim YJ (2006) Critical molecular switches involved in BMP-2-induced osteogenic differentiation of mesenchymal cells. 366:51–57

44.

Golub EE, Boesze-Battaglia K (2007) The role of alkaline phosphatase in mineralization. Curr Opin Orthop 18:444–448 CrossRef

45.

Birmingham E, Niebur GL, McHugh PE, Shaw G, Barry FP, McNamara LM (2012) Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche. Eur Cell Mater 23:13–27 CrossRefPubMed

46.

Martinasso G, Mozzati M, Pol R, Canuto RA, Muzio G (2007) Effect of superpulsed laser irradiation on bone formation in a human osteoblast-like cell line. Minerva Stomatol 56:27–30 PubMed

47.

Jawad M, Husein A, Azlina A, Alam MK, Hassan R, Shaari R (2013) Effect of 940 nm low-level laser therapy on osteogenesis in vitro. J Biomed Opt 18:1–6 CrossRef

48.

Titushkin I, Cho M (2009) Regulation of cell cytoskeleton and membrane mechanics by electric field: role of linker proteins. Biophys J 96:717–728 CrossRefPubMedPubMedCentral

49.

Mauney JR, Sjostorm S, Blumberg J, Horan R, O’Leary JP, Vunjak-Novakovic G, Volloch V, Kaplan DL (2004) Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds ın vitro. Calcif Tissue Int 74:458–468 CrossRefPubMed

Titel: Photostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source
Autoren:: Anıl Sera Çakmak
Soner Çakmak
H. Seda Vatansever
Menemşe Gümüşderelioğlu
Publikationsdatum: 18.12.2017
DOI: https://doi.org/10.1007/s10103-017-2414-4
Verlag: Springer London
Zeitschrift: Lasers in Medical Science
Ausgabe 4/2018
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X

Das kostenlose Testabonnement läuft nach 14 Tagen automatisch und formlos aus. Dieses Abonnement kann nur einmal getestet werden.

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

e.Dent - Das Online-Abo für Zahnärzte

Weitere Artikel der Ausgabe 4/2018

Effects of light-emitting diode irradiation on time to exhaustion at maximal aerobic speed

Direct biological effects of fractional ultrapulsed CO2 laser irradiation on keratinocytes and fibroblasts in human organotypic full-thickness 3D skin models

Photobiomodulation increases mitochondrial citrate synthase activity in rats submitted to aerobic training

Laser-induced autofluorescence-based objective evaluation of burn tissue repair in mice

Picosecond 532-nm neodymium-doped yttrium aluminium garnet laser—a novel and promising modality for the treatment of café-au-lait macules

In vitro outlook of gold nanoparticles in photo-thermal therapy: a literature review