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10.01.2022 | Original Article

Physicochemical and biological properties of experimental dental adhesives doped with a guanidine-based polymer: an in vitro study

verfasst von: Lucas Bonfanti Silvestrin, Isadora Martini Garcia, Fernanda Visioli, Fabrício Mezzomo Collares, Vicente Castelo Branco Leitune

Erschienen in: Clinical Oral Investigations | Ausgabe 4/2022

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Abstract

Objectives

The objective of this study is to formulate experimental dental adhesives with different polyhexamethylene guanidine hydrochloride concentrations (PHMGH) and evaluate their physical, chemical, and biological properties.

Materials and methods

The experimental adhesives were formulated with 0 (control, G_CTRL), 0.5 (G_0.5%), 1 (G_1%), or 2 (G_2%) wt.% into the adhesive. The adhesives were analyzed for degree of conversion (DC%), softening in solvent (ΔKHN%), ultimate tensile strength (UTS), microtensile bond strength (μTBS) immediately and after 1 year of aging, antibacterial activity, and cytotoxicity.

Results

There were no differences among groups for DC%, ΔKHN%, and UTS (p > 0.05%). There were no differences between each PHMGH-doped adhesive compared to G_CTRL in the immediate μ-TBS (p > 0.05). Adhesives with at least 1 wt.% of PHMGH presented better stability of μ-TBS. PHMGH-doped adhesives showed improved longitudinal μ-TBS compared to G_CTRL (p < 0.05). Lower Streptococcus mutans biofilm formation was observed for PHMGH-doped adhesives (p < 0.05). There was lower viability of planktonic S. mutans in the media in contact with the samples when at least 1 wt.% of PHGMGH was incorporated (p < 0.05). The formulated adhesives showed no cytotoxicity against pulp cells (p > 0.05).

Conclusions

The adhesive with 2 wt.% of PHMGH showed the highest antibacterial activity, without affecting the physicochemical properties and cytotoxicity, besides conferring stability for the dental adhesion.

Clinical relevance

PHMGH, a positively charged polymer, conveyed antibacterial activity to dental adhesives. Furthermore, it did not negatively affect the essential physicochemical and biocompatibility properties of the adhesives. More importantly, the incorporation of PHMGH provided stability for the μ-TBS compared to the control group without this additive.

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Titel: Physicochemical and biological properties of experimental dental adhesives doped with a guanidine-based polymer: an in vitro study
verfasst von: Lucas Bonfanti Silvestrin
Isadora Martini Garcia
Fernanda Visioli
Fabrício Mezzomo Collares
Vicente Castelo Branco Leitune
Publikationsdatum: 10.01.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 4/2022
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-021-04332-6

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Physicochemical and biological properties of experimental dental adhesives doped with a guanidine-based polymer: an in vitro study