Skip to main content
Erschienen in: Inflammopharmacology 2/2024

06.02.2024 | Original Article

Phytochemical analysis and immunomodulatory activities in vitro and in vivo of Aframomum melegueta K Schum seed extracts

verfasst von: Mounia Latif, Ismail Elkoraichi, Othman El Faqer, Hicham Wahnou, El Mostafa Mtairag, Mounia Oudghiri, Samira Rais

Erschienen in: Inflammopharmacology | Ausgabe 2/2024

Einloggen, um Zugang zu erhalten

Abstract

Aframomum melegueta K Schum (A. melegueta), an herbaceous plant renowned for its medicinal seeds, was investigated for its potential immunomodulatory effects in vitro and in vivo using ethanolic and methanolic extracts. The immunomodulatory effect was evaluated by measuring antibody titers using the agglutination technique, while anti-inflammatory activity was assessed in a carrageenan-induced mouse paw edema model. In vitro immunomodulatory activity was measured by lysozyme release from neutrophils. Additionally, white blood cell counts were analyzed post-extracts treatment. The MTT assay was employed to determine cytotoxicity, and the biochemical parameters of liver toxicity were evaluated. Remarkably, both extracts exhibited a dose-dependent reduction in paw edema (p < 0.001), with the most significant reduction observed at 1 g/kg (78.13 and 74.27% for ethanolic and methanolic extracts, respectively). Neutrophil degranulation was significantly inhibited in a dose-dependent manner (p < 0.003), reaching maximal inhibition at 100 μg/mg (60.78 and 39.7% for ethanolic and methanolic extracts, respectively). In comparison to the control group, both antibody production and white blood cell counts were reduced. Neither of the extracts showcased any cytotoxicity or toxicity. These findings suggest that A. melegueta extracts exhibit immunosuppressive and anti-inflammatory activities due to the presence of various biomolecules.
Literatur
Zurück zum Zitat Agim C, Nosiri C, Anyanwu C and Atasie O (2017). Evaluation of the phytochemical and GC-MS analysis of the aqueous seed extract of Aframomum melegueta. J Pharmacogn Phytochem Agim C, Nosiri C, Anyanwu C and Atasie O (2017). Evaluation of the phytochemical and GC-MS analysis of the aqueous seed extract of Aframomum melegueta. J Pharmacogn Phytochem
Zurück zum Zitat Asiat N, Yusuf Oloruntoyin A, Damilola Ibukun K, Sobiat S, Bukola B, Mutiu Adewunmi A, Abdul-Azeez B, Azeemat Titilola A, Mohd Nizam M (2020) Phytochemical screening and in silico pharmacological profiling of ethanolic extract of Aframomum melegueta for prostate carcinoma. J Appl Pharma Sci. https://doi.org/10.7324/JAPS.2021.110715CrossRef Asiat N, Yusuf Oloruntoyin A, Damilola Ibukun K, Sobiat S, Bukola B, Mutiu Adewunmi A, Abdul-Azeez B, Azeemat Titilola A, Mohd Nizam M (2020) Phytochemical screening and in silico pharmacological profiling of ethanolic extract of Aframomum melegueta for prostate carcinoma. J Appl Pharma Sci. https://​doi.​org/​10.​7324/​JAPS.​2021.​110715CrossRef
Zurück zum Zitat Diasio RB, and LoBuglio AF (1996) Immunomodulators : Immuno-suppressive agents and immunostimulants. 1291–1307. Diasio RB, and LoBuglio AF (1996) Immunomodulators : Immuno-suppressive agents and immunostimulants. 1291–1307.
Zurück zum Zitat Gbadamosi IT, Moody JO, Lawal AM (2011) Phytochemical screening and proximate analysis of eight ethnobotanicals used as antimalaria remedies in Ibadan Nigeria. J Appl Biosci. 44:2967–2971 Gbadamosi IT, Moody JO, Lawal AM (2011) Phytochemical screening and proximate analysis of eight ethnobotanicals used as antimalaria remedies in Ibadan Nigeria. J Appl Biosci. 44:2967–2971
Zurück zum Zitat Kamtchouing P, Mbongue GYF, Dimo T, Watcho P, Jatsa HB, Sokeng SD (2002) E¡ects of A. melegueta and Piper guineense on sexual behaviour of male rats. Behav Pharmacol. 5:243–247CrossRef Kamtchouing P, Mbongue GYF, Dimo T, Watcho P, Jatsa HB, Sokeng SD (2002) E¡ects of A. melegueta and Piper guineense on sexual behaviour of male rats. Behav Pharmacol. 5:243–247CrossRef
Zurück zum Zitat Katawa G, Ataba E, Ritter M, Amessoudji OM, Awesso ER, Tchadié PE, Bara FD, Douti FV, Arndts K, Tchacondo T, Batawila K, Ameyapoh Y, Hoerauf A, Karou SD, Layland LE (2022) Anti-Th17 and anti-Th2 responses effects of hydro-ethanolic extracts of Aframomum melegueta, Khaya senegalensis and Xylopia aethiopica in hyperreactive onchocerciasis individuals’ peripheral blood mononuclear cells. PLoS Negl Trop Dis 16(4):e0010341. https://doi.org/10.1371/journal.pntd.0010341CrossRefPubMedPubMedCentral Katawa G, Ataba E, Ritter M, Amessoudji OM, Awesso ER, Tchadié PE, Bara FD, Douti FV, Arndts K, Tchacondo T, Batawila K, Ameyapoh Y, Hoerauf A, Karou SD, Layland LE (2022) Anti-Th17 and anti-Th2 responses effects of hydro-ethanolic extracts of Aframomum melegueta, Khaya senegalensis and Xylopia aethiopica in hyperreactive onchocerciasis individuals’ peripheral blood mononuclear cells. PLoS Negl Trop Dis 16(4):e0010341. https://​doi.​org/​10.​1371/​journal.​pntd.​0010341CrossRefPubMedPubMedCentral
Zurück zum Zitat Kouar J, Lamsaddek A, Benchekroun R, El Amrani A, Cherif A, OuldBellahcen T, Kamil N (2019) Comparison between electrocoagulation and solvent extraction method in the process of the dechlorophyllation of alcoholic extracts from Moroccan medicinal plants Petroselinum crispum, Thymus satureioides and microalgae Spirulina platensis. SN Appl Sci 1(1):132. https://doi.org/10.1007/s42452-018-0137-1CrossRef Kouar J, Lamsaddek A, Benchekroun R, El Amrani A, Cherif A, OuldBellahcen T, Kamil N (2019) Comparison between electrocoagulation and solvent extraction method in the process of the dechlorophyllation of alcoholic extracts from Moroccan medicinal plants Petroselinum crispum, Thymus satureioides and microalgae Spirulina platensis. SN Appl Sci 1(1):132. https://​doi.​org/​10.​1007/​s42452-018-0137-1CrossRef
Zurück zum Zitat Omotuyi IO, Nash O, Ajiboye BO, Olumekun VO, Oyinloye BE, Osuntokun OT, Olonisakin A, Ajayi AO, Olusanya O, Akomolafe FS, Adelakun N (2021) A. melegueta secondary metabolites exhibit polypharmacology against SARS-CoV-2 drug targets: in vitro validation of furin inhibition. Phytother Res 35(2):908–919. https://doi.org/10.1002/ptr.6843CrossRefPubMed Omotuyi IO, Nash O, Ajiboye BO, Olumekun VO, Oyinloye BE, Osuntokun OT, Olonisakin A, Ajayi AO, Olusanya O, Akomolafe FS, Adelakun N (2021) A. melegueta secondary metabolites exhibit polypharmacology against SARS-CoV-2 drug targets: in vitro validation of furin inhibition. Phytother Res 35(2):908–919. https://​doi.​org/​10.​1002/​ptr.​6843CrossRefPubMed
Zurück zum Zitat Shantilal S, Vaghela JS, Sisodia SS (2018) Review on immunomodulation and immunomulatory activity of some medicinal plants. European J Biomed Pharm Sci 5:163–174 Shantilal S, Vaghela JS, Sisodia SS (2018) Review on immunomodulation and immunomulatory activity of some medicinal plants. European J Biomed Pharm Sci 5:163–174
Zurück zum Zitat Toh EYS, Lim CL, Ling APK, Koh RY (2019) Overview of the pharmacological activities of aframomum melegueta. J Trop Agric Sci 42:1–13 Toh EYS, Lim CL, Ling APK, Koh RY (2019) Overview of the pharmacological activities of aframomum melegueta. J Trop Agric Sci 42:1–13
Zurück zum Zitat Xu B, Ganesan K, Mickymaray S, Alfaiz FA, Thatchinamoorthi R, Aboody MSA (2020) Immunomodulatory and antineoplastic efficacy of common spices and their connection with phenolic antioxidants. Bioactive Compd Health Dis 3(2):15 Xu B, Ganesan K, Mickymaray S, Alfaiz FA, Thatchinamoorthi R, Aboody MSA (2020) Immunomodulatory and antineoplastic efficacy of common spices and their connection with phenolic antioxidants. Bioactive Compd Health Dis 3(2):15
Zurück zum Zitat Yadav SP, Kannaki TR, Mahapatra RK, Paswan C, Bhattacharya TK, Sarkar SK, Chatterjee RN (2018) In vivo cell-mediated immune, hemagglutination inhibition response, hematological and biochemical values in native vs exotic chicken breeds. Poult Sci 97(9):3063–3071. https://doi.org/10.3382/ps/pey182CrossRefPubMed Yadav SP, Kannaki TR, Mahapatra RK, Paswan C, Bhattacharya TK, Sarkar SK, Chatterjee RN (2018) In vivo cell-mediated immune, hemagglutination inhibition response, hematological and biochemical values in native vs exotic chicken breeds. Poult Sci 97(9):3063–3071. https://​doi.​org/​10.​3382/​ps/​pey182CrossRefPubMed
Metadaten
Titel
Phytochemical analysis and immunomodulatory activities in vitro and in vivo of Aframomum melegueta K Schum seed extracts
verfasst von
Mounia Latif
Ismail Elkoraichi
Othman El Faqer
Hicham Wahnou
El Mostafa Mtairag
Mounia Oudghiri
Samira Rais
Publikationsdatum
06.02.2024
Verlag
Springer International Publishing
Erschienen in
Inflammopharmacology / Ausgabe 2/2024
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI
https://doi.org/10.1007/s10787-023-01422-7

Weitere Artikel der Ausgabe 2/2024

Inflammopharmacology 2/2024 Zur Ausgabe