Background
Anti-malarial drug resistance is a major impediment to malaria chemotherapy in sub-Saharan Africa [
1] largely because
Plasmodium falciparum rapidly develops resistance to drugs [
2]. Resistance to anti-malarial drugs occurs through drug-selection of spontaneous mutations in
P. falciparum that confer tolerance to the drug [
3]. The selection and spread of drug resistant
P. falciparum is facilitated by the rapid genome replication rate and by a relatively high mutation rate per generation of the parasite [
4,
5]. The speed of selection of mutants within parasite populations depends upon the pharmacokinetics of the drug itself and its degree of usage within a given host population [
1]. For many anti-malarial drugs, molecular markers of parasite resistance are known. Surveillance of these markers in parasite populations can act as a proxy measure of the efficacy of drugs within that population, and can act as early warning signals of the emergence of resistance into new regions. Frequent and thorough molecular surveys of the prevalence of mutations associated with drug resistance can, therefore, inform regional drug policies.
Single nucleotide polymorphisms (SNPs) in the
P. falciparum multidrug resistance gene (
pfmdr1) have been shown to modulate the susceptibility of the parasite to the long acting partner drug in Artemisinin-based combination therapy (ACT) [
6], but are not associated with resistance to artemisinin. Artemether-lumefantrine (AL) and artesunate-amodiaquine (AS-AQ) are two combinations commonly used in ACT in sub-Saharan Africa to treat uncomplicated malaria, but until now, there is no clear evidence of treatment failure from their use in the region. However, some genetic studies involving
pfmdr1 have suggested opposing selective pressures following separate use of the drugs, in which parasites harbouring N86, 184F, D1246
pfmdr1 genotypes predominate in African countries that recommend AL as first line anti-malarial drug, whilst those carrying 86Y, Y184 and 1246Y
pfmdr1 genotypes predominate in African countries that use AS-AQ as frontline anti-malarial therapy [
7].
African
P. falciparum isolates may carry the resistant allele of
pfcrt encoding the amino acids CVIET at codons 72–76 as well as a variety of polymorphic
pfmdr1 alleles which have originated and spread within the African continent [
8‐
10]. The
pfmdr1 gene is a structural homologue of the mammalian multidrug resistance gene encoding a P-glycoprotein homologue-1 (Pgh1) multi-drug resistant transporter [
11] and is expressed into a
PfMDR1 transporter located in the
P. falciparum food vacuole.
Mutations in
pfmdr1 are associated with reduced influx of diverse anti-malarial drugs reducing their intracellular accumulation [
12,
13]. Single nucleotide polymorphisms (SNPs) in
pfmdr1 are associated with resistance to aminoquinolines [
14,
15]. Several codons in
pfmdr1 have been putatively linked with changes in the parasite’s susceptibility to anti-malarial drugs, but codons N86Y, Y184F and D1246Y are uniquely associated with changes in sensitivity to lumefantrine (LUM) and amodiaquine (AQ) in sub-Saharan Africa [
16]. While the
pfmdr1 86Y allele was strongly associated with chloroquine (CQ) and amodiaquine (AQ) resistance [
17,
18], 1246Y alleles were shown to confer resistance to quinine (QN) and possess the capacity to
increase the parasite susceptibility to mefloquine (MQ), halofantrine (HF) and artemisinin (ART) [
19,
20]. In the study of Reed et al. [
21], the sensitivity of CQS D10 parasites to CQ was not affected by transfection of the parasites with
pfmdr1 D1246Y mutation, but reduced by half, due to replacement of the mutation with a wild-type D10
pfmdr1 sequence on a different genetic background of the parasite (CQR 7G8).
The mutant
pfmdr1 86Y and 1246Y alleles have also been linked to reduced sensitivity to AQ, whereas the wild-type
pfmdr1 N86 and D1246 alleles are linked to reduced susceptibility against LUM [
22,
23]. In Africa, the common use of AL and AS-AQ in the treatment of uncomplicated malaria has been linked with the emergence of
pfmdr1 N86Y, Y184F and D1246Y SNPs [
24], and the prevalence of these mutations are frequently used for evaluating changes in sensitivity to LUM and AQ partners in artemisinin-based combinations [
7]. Several studies have shown that parasites carrying a combination of
pfmdr1 N86, 184F, and D1246 (the “NFD” haplotype) display decreased susceptibility to AL and that treatment with AL can select for such a haplotype [
25,
26].
Nigeria accounts for 25% of global cases of malaria and an estimated 50% of the country’s population suffer at least one episode of malaria every year, while under-five children experience an average of 2 to 4 attacks in a year [
27].
Plasmodium falciparum is stably and perennially transmitted in all parts of the country [
28], with transmission increased during the wet season compared to the dry [
29,
30]. North-West and North–East Nigeria have so far been identified as hotspots of malaria in relation to the southern parts of the country due primarily to climatic and environmental conditions [
31]. The North-West region of the country suffers a much higher
P. falciparum transmission rate than the other regions including North-East Nigeria [
32].
The frontline drug for malaria chemotherapy in the country was chloroquine until 2005 when it was withdrawn as a result of resistance [
33]. Subsequently, the artemether-lumefantrine was recommended as the only first-line drug for the treatment of uncomplicated malaria in the regions. Unfortunately, several reports investigating molecular markers of anti-malarial resistance have suggested a massive reduction of parasite susceptibility to LUM component of AL [
19,
24,
34,
35]. In Uganda, Dokomajilar et al. [
34] showed a high prevalence of
pfmdr1 N86, 184F, and D1246 alleles after treatment with AL, where the pattern persists even in patients that presented with clinical failure. A few years after, Mbogo et al. [
24] genotyped 982 archived samples collected during 2003–2012 for
pfmdr1 polymorphisms and reported a dramatic reduction in
pfmdr1 86Y and 1246Y alleles over time. Similarly, the relationship between presence of mutations involving
pfmdr1 86, 184 and 1246 codons and success of ultralow-dose mefloquine treatment was investigated in Gabon, where Mawili-Mboumba et al. [
35] observed a low prevalence of
pfmdr1 N86 allele but the prevalence of 184F and D1246 alleles was above 80% each. In Tanzania, Humphreys et al. [
19] observed a high prevalence of
pfmdr1 86Y, Y184 and 1246Y in patients who failed treatment with AQ, but observed the opposite in those who failed AL treatment. The prevalence of
pfmdr1 polymorphisms in Nigeria was majorly reported from the Southern region, where a positive association between
pfmdr1 N86, F184 and D1246 alleles and clinical failure was observed [
36]. In contrast, a prevalence of 62.2% and 69.0% for
pfmdr1 86Y and F184 allele, respectively, was also reported from the region [
37] which was recently followed by another survey where the
pfmdr1 86Y and 1246Y alleles had prevalence of 24% and 18.6%, respectively [
38]. Yet, there is no valid baseline data involving
pfmdr1 SNPs in both North-West and North–East Nigeria since the withdrawal of CQ and adoption of AL in Northern Nigeria. In this study, the distributions of the
pfmdr1 N86Y, Y184F and D1246Y SNPs across the North-West and-East Nigeria were investigated.
Discussion
Mutations in several genes, including
pfmdr1,
pfcrt and
pfk13 are associated with variation in parasite sensitivity to a range of drugs [
12,
42,
43]. The
pfmdr1 mutations N86Y, Y184F and D1246Y SNPs are thought to modulate susceptibility to CQ, AL and AS-AQ [
6]. It was observed that,
pfmdr1 184F and 86Y alleles predominated in North-West Nigeria while 1246Y was higher in the North-East.
Alleles of
pfmdr1 carrying the wild type N86 residue are associated with higher IC
50 and IC
90 values for LMF, MFQ and DHA, while the alternative 86Y residue seems to confer increased resistance against CQ and AQ [
6]. Similarly, there are varying epidemiological reports on the prevalence and consequences of
pfmdr1 N86Y polymorphisms from different parts of the world. For example, Ibraheem et al. [
44] suggested that
pfmdr1 mutations are geographically confined and have inconsistent distributions from one geographic region to another.
Following adoption of ACT in many African countries, some studies from West Africa have linked the prevalence of the
pfmdr1 N86 allele to selection by AL [
7]. Therefore, the high prevalence of
pfmdr1 N86 allele observed in the present study, even with the absence of clinical data of patients, might be suggestive of possible AL pressure in all the states. In addition, the finding might also suggest that the efficacy of the LMF component of ACT is susceptible to the emergence of tolerance in the local
P. falciparum populations, as the presence of
pfmdr1 N86 is critical in the initiation of resistance to LMF in vivo and that its selection primarily follows reinfection and recrudescence events associated with the elimination stage of LMF, 4–5 days after artemether clearance [
45].
Some reports have associated a rise in the prevalence of
pfmdr1 86Y alleles with increasing CQ resistance [
14,
46,
47]. The low prevalence of
pfmdr1 86Y in Adamawa and Yobe raises the possibility that CQ may be effective against
P. falciparum malaria in North-Eastern Nigeria once again, although this would be presumably tempered by CQ-resistance associated mutations in
pfcrt, which was not assayed here. It is possible that the selection of
pfmdr1 86Y allele in this region was aided by the cessation of CQ usage due to the emergence of resistance. The high prevalence of this mutation across Northern Nigeria may indicate that the efficacy of AL is at risk in this region, but raises the possibility that CQ may be effective in the chemotherapy of uncomplicated malaria here.
The
pfmdr1 Y184F polymorphisms have been reported by various in vitro studies to be weakly associated with changes in anti-malarial drug response [
6,
12,
48]. The IC
50 of some anti-malarial drugs was shown to vary on the sole acquisition of either
pfmdr1 N86 or 86Y alleles by parasite lines expressing wild type
pfmdr1-Y184 or mutant 184F alleles [
6]. Several epidemiological studies on the prevalence of
pfmdr1 Y184F polymorphisms have shown that the Y184 allele is predominantly confined to East and Central Africa while the mutant 184F allele predominates in West Africa [
7,
49‐
51]. Okell et al. [
7] observed higher occurrence of
pfmdr1 184F in West Africa than East and Central Africa after extracting data from 397 surveys measuring the prevalence or frequency of at least one
pfmdr1 polymorphism in 30 countries. In Cameroon, the prevalence of
pfmdr1 184F allele was shown to reduce drastically from 97.3 to 56% in 2003–2013 [
50]. Achieng et al. [
49] showed that between 1995 and 2003 prior to the introduction of ACT in Kenya, the prevalence of
pfmdr1 Y184 was 100%, but declined to 99.3% between 2008 and 2014 post ACT introduction. In a study conducted in Ghana by Duah et al. [
52] using archived filter paper blood spots from under-five children with uncomplicated malaria in 2003–2010, the prevalence of
pfmdr1 184F was reported to increase steadily as 26–78%, 35–82%, 48–70%, and 40–80% for 2003–2004, 2005–2006, 2007–2008, and 2010, respectively. Indeed, reports of the high occurrence of the mutant
pfmdr1 184F in West Africa were corroborated by the present findings in which it was observed that the prevalence of
pfmdr1 184F was high in all the states, and especially in Kano, and that its prevalence is higher in North-West compared to North-East Nigeria. This mutation has been previously linked to a reduction in susceptibility to LUM and/or ART [
53]. It is perhaps unsurprising that a relatively high prevalence of this mutation was found in regions, such as Tanzania in East Africa, where AL is first-line intervention against uncomplicated malaria. Thus, the high prevalence of mutant
pfmdr1 184F obtained in the present study may simply reflect the relatively common use of AL treatment in this region.
The
pfmdr1 D1246Y mutation affects
P. falciparum susceptibility to various anti-malarials including QN, MFQ, (HF), CQ and ART, with the latter two drugs affected in a strain specific manner [
16,
21]. The observed low prevalence of mutant
pfmdr1 1246Y alleles compared to the wild type in this study is consistent with reports from Southern Nigeria [
37] as well as other West and East African countries that adopted AL as a front-line anti-malarial therapy for uncomplicated malaria [
7,
49]. Countries in Central Africa have observed an unsteady increase in the prevalence of the
pfmdr1 D1246 allele, possibly due to the selective pressure of AS-AQ [
42,
54].
Several reports from Africa have suggested that linkage between
pfmdr1 N86Y/Y184F/D1246Y results in haplotypes with particular phenotypic characteristics that may be selected depending on the particular drugs that the population is exposed to [
55,
56]. The occurrence of
pfmdr1 NFD and NYD haplotypes, for example, may result from AL selection while the
pfmdr1 YYY haplotype may be favoured in regions where parasites are exposed to AS-AQ, DHAP and CQ [
12,
50]. The treatment of uncomplicated malaria with AL often selects
pfmdr1 haplotypes bearing the N86 allele [
51,
57]. A predominance of the
pfmdr1 NFD haplotype was found in Northern Nigeria with Kano, and a complete absence of the
pfmdr1 YYY haplotype. These findings are in line with the selective effect of AL on the NFD haplotype. Hence, the abundance of
pfmdr1 NFD haplotype may suggest a loss of susceptibility to AL treatment by the parasites in the region. Resistance associated SNPs in
pfk13 should be analysed in a subsequent follow-up study, so as to confirm the influence of
pfmdr1 NFD haplotype on reduced AL sensitivity in the regions.
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