The authors declare that they have no competing interests.
JFG, TAAC conceived and designed the experiments; TAAC, MGQ, GLC, IGD performed the experiments; JFG analysed the data wrote the paper; JFG, TAAC, MGQ, AYNP carried out the biological material and data collection in the field. All authors read and approved the final manuscript.
There is large body of evidence that states that invasion of Plasmodium vivax requires the Duffy antigen, but the universality of this specificity is certainly now under question with recent reports showing that in some parts of the world P. vivax infects and causes disease in Duffy-negative people. These findings reinforce the idea that this parasite is rapidly evolving, being able to use other receptors than Duffy to invade the erythrocytes, which may have an enormous impact in P. vivax current distribution. The presence of P. vivax infection in Duffy-negative individuals was investigated in a cross-sectional study conducted in Anajás, Archipelago of Marajó, State of Pará, which is an area of malaria transmission in the Brazilian Amazonia.
Duffy genotyping and Plasmodium species diagnostic assays were performed successfully in 678 individuals. An allele-specific primer polymerase chain reaction (PCR) technique was used for Duffy blood group genotyping. Identification of Plasmodium species was achieved by conventional blood smear light microscopy and a TaqMan-based real-time PCR method to detect mitochondrial genome of Plasmodium falciparum and P. vivax.
Plasmodium spp. infection was detected in 137 samples (20.2%). Prevalence of each Plasmodium species was 13.9% P. vivax, 5.8% P. falciparum, and 0.6% P. vivax plus P. falciparum. Overall, 4.3% (29/678) were genotyped as Duffy-negative (FY*B ES /*B ES ). Among Duffy-negative individuals 6.9% were P. vivax PCR positive and among Duffy-positive 14.2% were P. vivax PCR positive. Although lower, the risk of Duffy-negatives to experience a P. vivax blood stage infection was not significantly different to that of Duffy-positives. Furthermore, the genotypic and allelic frequencies of the Duffy blood group among P. vivax-infected patients and in the control group did not differ significantly, also suggesting no reduction in infection rates among the carriers of FY*B ES allele.
The data obtained in Anajás showed no differential resistance vivax malaria among Duffy-negative and Duffy-positive individuals. This result needs additional confirmation through a deeper evaluation in a larger sample of patients with P. vivax malaria and molecular parasite characterization. Nonetheless, this genetic profile of the parasite may be contributing to the high incidence of malaria in the municipality.
Oliveira-Ferreira J, Lacerda MVG, Brasil P, Ladislau JLB, Tauil PL, Daniel-Ribeiro CT: Malaria in Brazil: an overview. Malar J. 2010, 115: 115-128. CrossRef
Ministério da Saúde: Sistema de Informação e Vigilância Epidemiológica da Malária. SIVEP/Malária. http://portalweb04.saude.gov.br/sivep_malaria/default.asp,
Chaudhuri A, Polyakova J, Zbrzezna V, Pogo AO: The coding sequence of Duffy blood group gene in humans and simians: restriction fragment length polymorphism, antibody and malarial parasite specificities, and expression in nonerythroid tissues in Duffy-negative individuals. Blood. 1995, 85: 615-621. PubMed
Iwamoto S, Omi T, Kajii E, Ikemoto S: Genomic organization of the glycoprotein D gene: Duffy blood group Fya/Fyb alloantigen system is associated with a polymorphism at the 44-amino acid residue. Blood. 1995, 85: 622-626. PubMed
Mallinson G, Soo KS, Schall TJ, Pisacka M, Anstee DJ: Mutation in the erythrocyte chemokine receptor (Duffy) gene: the molecular basis of the Fya/Fyb antigens and identification of a deletion in the Duffy gene of an apparently healthy individual with the Fy(a-b-) phenotype. Brit J Hematol. 1995, 90: 823-829. 10.1111/j.1365-2141.1995.tb05202.x. CrossRef
Kasehagen LJ, Mueller I, Kiniboro B, Bockarie MJ, Reeder JC, Kazura JW, Kastens W, McNamara DT, King CH, Whalen CC, Zimmerman PA: Reduced Plasmodium vivax erythrocyte infection in PNG Duffy-negative heterozygotes. PLoS One. 2007, 2: 336-10.1371/journal.pone.0000336. CrossRef
Ryan JR, Stoute JA, Amon J, Dunton RF, Mtalib R, Koros J, Owour B, Luckhart S, Wirtz RA, Barnwell JW, Rosenberg R: Evidence for transmission of Plasmodium vivax among a Duffy antigen negative population in Western Kenya. Am J Trop Med Hyg. 2006, 75: 575-581. PubMed
Cavasini CE, Mattos LC, Couto AA, Bonini-Domingos CR, Valencia SH, Neiras WC, Alves RT, Rossit AR, Castilho L, Machado RL: Plasmodium vivax infection among Duffy antigen negative individuals from the Brazilian Amazon region: an exception?. Trans R Soc Trop Med Hyg. 2007, 101: 1042-1044. 10.1016/j.trstmh.2007.04.011. CrossRefPubMed
Cavasini CE, Mattos LC, Couto AA, Bonini-Domingos CR, Valencia SH, Neiras WC, Alves RT, Rossit AR, Castilho L, Machado RL: Duffy blood group gene polymorphisms among malaria vivax patients in four areas of the Brazilian Amazon region. Malar J. 2007, 6: 167-10.1186/1475-2875-6-167. PubMedCentralCrossRefPubMed
Ménard D, Barnadas C, Bouchier C, Henry-Halldin C, Gray R, Ratsimbasoa A, Thoniera V, Carodf JF, Domarlea O, Coling Y, Bertrandg O, Picotg J, King CL, Grimbergc BT, Mercereau-Puijalonb O, Zimmerman PA: Plasmodium vivax clinical malaria is commonly observed in Duffy-negative Malagasy people. Proc Natl Acad Sci USA. 2010, 107: 5967-5971. 10.1073/pnas.0912496107. PubMedCentralCrossRefPubMed
Mendes C, Dias F, Figueiredo J, Mora VG, Cano J, Sousa B, Rosário VE, Benito A, Berzosa P, Arez AP: Duffy negative antigen is no longer a barrier to Plasmodium vivax – molecular evidences from the African West Coast (Angola and Equatorial Guinea). PLoS Negl Trop Dis. 2011, 5: e1192-10.1371/journal.pntd.0001192. PubMedCentralCrossRefPubMed
Doenças de A a Z: 2010, http://portal.saude.gov.br/portal/saude/profissional/areacfm?id_area=1526,
Manual de diagnóstico laboratorial da malária. 2005, http://portal.saude.gov.br/portal/arquivos/pdf/manual_diagnostico_malaria.pdf,
Olsson ML, Hansson C, Avent ND, Akesson IE, Green CA, Daniels GL: A clinically applicable method for determining the three major alleles at the Duffy (FY) blood group locus using polymerase chain reaction with allele-specific primers. Transfusion. 1998, 38: 168-173. 10.1046/j.1537-2995.1998.38298193099.x. CrossRefPubMed
King CL, Adams JH, Xianli J, Grimberg BT, McHenry AM, Greenberg LJ, Siddiqui A, Howes RE, da Silva-Nunes M, Ferreira MU, Zimmerman PA: Fy(a)/Fy(b) antigen polymorphism in human erythrocyte Duffy antigen affects susceptibility to Plasmodium vivax malaria. Proc Natl Acad Sci USA. 2011, 108: 20113-20118. 10.1073/pnas.1109621108. PubMedCentralCrossRefPubMed
Perna SJQ, Cardoso GL, Guerreiro JF: Duffy blood group genotypes among African Brazilian communities of the Amazon region. Genet Mol Res. 2007, 6: 166-172. PubMed
Cavasini CE, Pereira FJT, Ribeiro WL, Wunderlich G, Ferreira MU: Duffy blood group genotypes among malaria patients in Rondônia, Western Brazilian Amazon. Rev Soc Bra Med Trop. 2001, 34: 591-595. 10.1590/S0037-86822001000600016. CrossRef
Santos SEB, Santos AKCR, Santos EJM, Guerreiro JF: The Amazon microcosm. Ciência e Cultura. 1999, 51: 181-190.
- Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy-negative and Duffy-positive individuals
Tarcisio AA Carvalho
Maíse G Queiroz
Greice L Cardoso
Isabela G Diniz
Aylla NLM Silva
Ana YN Pinto
João F Guerreiro
- BioMed Central
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