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Inflammopharmacology

Erschienen in:

08.01.2018 | Original Article

Polar extract of Curcuma longa protects cartilage homeostasis: possible mechanism of action

verfasst von: Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard, Bharathi Bethapudi

Erschienen in: Inflammopharmacology | Ausgabe 5/2018

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Abstract

Background

Curcuma longa has been well documented for managing joint inflammation and pain. The present study investigated the effect of polar extract of C. longa (NR-INF-02) on cartilage homeostasis in human articular chondrocytes knee (NHAC-kn) cells to understand its plausible mechanism of action.

Methods

Dysregulation of cartilage homeostasis was induced by IL-1β and H₂O₂. Modulating effects of NR-INF-02 on degradation markers viz., chondrocyte apoptosis, senescence, cytokine, eicosanoids, and cartilage synthesis markers viz., glycosaminoglycans and type II collagen degradation was evaluated in human articular chondrocytes knee (NHAC-kn) cells. Further, the effect of NR-INF-02 on lipopolysaccharide (LPS)-induced expression of NF-kB in RAW264.7 macrophages was investigated.

Results

NR-INF-02 significantly attenuated IL-1β-induced chondrocyte cytotoxicity, apoptosis and release of chondrocyte degradation markers such as IL-6, IL-8, COX-2, PGE₂, TNF-α, ICAM-1 in NHAC-kn cells. Also, NR-INF-02 protected IL-1β-induced damage to synthesis markers such as glycosaminoglycans, type II collagen and further attenuated H₂O₂-induced chondrocyte senescence. In addition NR-INF-02 suppressed LPS-induced NF-kB expression in RAW264.7 cells.

Conclusions

NR-INF-02 protects cartilage homeostasis by maintaining the balance between synthesis and degradation of cartilage matrix.

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Titel: Polar extract of Curcuma longa protects cartilage homeostasis: possible mechanism of action
verfasst von: Chandrasekaran Chinampudur Velusami
Edwin Jothie Richard
Bharathi Bethapudi
Publikationsdatum: 08.01.2018
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 5/2018
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-017-0433-1

Springer Medizin

Abstract

Background

Methods

Results

Conclusions

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