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01.12.2015 | Research article | Ausgabe 1/2015 Open Access

BMC Musculoskeletal Disorders 1/2015

Polymorphic variation of hypoxia inducible factor-1 A (HIF1A) gene might contribute to the development of knee osteoarthritis: a pilot study

BMC Musculoskeletal Disorders > Ausgabe 1/2015
Javier Fernández-Torres, Cristina Hernández-Díaz, Rolando Espinosa-Morales, Javier Camacho-Galindo, Norma del Carmen Galindo-Sevilla, Ámbar López-Macay, Yessica Zamudio-Cuevas, Karina Martínez-Flores, Mónica Guadalupe Santamaría-Olmedo, Carlos Pineda, Julio Granados, Gabriela Angélica Martínez-Nava, Marwin Gutiérrez, Alberto G. López-Reyes
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12891-015-0678-z) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

JFT and ALR participate in study coordination, responsibility for the integrity of the work, interpretation of data, drafted the manuscript, and gave final approval of the version of the paper to be published. CHD, REM, JCG, NGS, YZC, KMF and MSO, collected and interpretation of data, drafted the manuscript. ALM, GAMN and MG performed the statistical analysis, interpretation of data, and help to draft the manuscript. CP and JG help to draft and revise the manuscript, interpretation of data. All authors read and approved the final version of the manuscript.



Osteoarthritis (OA) is a multifactorial degenerative condition of the whole joint with a complex pathogenesis whose development and progression is significantly mediated by interactions between the joint cartilage and articular tissues, particularly, proinflammatory mediators and oxidative stress, which results in cartilage deterioration and subchondral bone destruction. HIF-1 alpha regulates oxygen homeostasis in hypoxic tissues such as joint cartilage; efficiency of transcriptional activity of the HIF1A gene is strongly influenced by the presence of polymorphic variants. Given the loss of articular cartilage and with intention to restore damaged tissue, WISP-1 participates in the development of subchondral bone; further, its expression is highly increased in chondrocytes of OA patients. The aim of this study was to evaluate gene frequencies of HIF1A and WISP1 polymorphisms in Mexican patients suffering from knee OA.


We determined HIF1A rs11549465 (P582S), rs11549467 (A588T), and rs2057482 (C191T), and WISP1 rs2929970 (A2364G) polymorphisms in 70 Mexican patients with knee OA and compare them to those present in 66 ethnically matched healthy controls. Genotyping for these polymorphisms was performed by Real-Time PCR using TaqMan probes.


Gene frequencies exhibited a significant increase of the CC genotype of rs11549465 polymorphism in knee OA patients as compared with those present in controls (P = 0.003 OR = 5.7, 95 % CI = 1.7–21.6); CT genotype and T allele showed decreased frequency in the knee OA group vs. the controls (P = 0.003 OR = 0.2, CI = 0.05–0.6; and P = 0.004 OR = 0.2, CI = 0.05–0.65, respectively). Allele frequencies of the other polymorphic variants were similar in both patients and controls.


These results suggest that the presence of the rs11549465 SNP (HIF1A) plays a role protective in the loss of articular cartilage in our population, and offers the possibility to further study the molecular mechanisms within cartilage and subchondral bone.
Additional file 1: Gene and allele frequencies of the rs11549465 polymorphism in OA patients according the Kellgren-Lawrence scale. (DOC 42 kb)
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